Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq

doi:10.1016/S2095-3119(18)61956-2

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (07): 1574-1584.DOI: 10.1016/S2095-3119(18)61956-2

收稿日期:2017-12-21 出版日期:2018-07-04 发布日期:2018-07-05

Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq

ZHOU Yu-qian^1*, WANG Qin-yang^2*, ZHAO Hai-liang², GONG Dian-ming², SUN Chuan-long², REN Xue-mei², LIU Zhong-xiang¹, HE Hai-jun¹, QIU Fa-zhan²

¹Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China
² National Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China

Received:2017-12-21 Online:2018-07-04 Published:2018-07-05
Contact: Correspondence QIU Fa-zhan, Tel: +86-27-87286870, Fax: +86-27-87281006, E-mail: qiufazhan@mail.hzau.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This research was supported by the National Natural Science Foundation of China (91735306), the National Basic Research Program of China (973 Program, 2014CB138203), and the National Key Research and Development Program of China (2016YFD0100103-19).

摘要/Abstract

Abstract:

Seed size play a significant role in maize yield production. Two maize inbred lines with distinct seed size and weight, V671 (a large-seed inbred line) and Mc (a small-seed inbred line), were investigated by RNA-seq at 14 days after pollination (DAP), when maize endosperm undergoes an active transition from mitosis to storage accumulation. RNA-seq expression data showed that the small-seed line Mc had a higher storage accumulation activity, whereas the large-seed kernel line V671 possessed a higher DNA synthesis activity. An investigation of the pattern of increasing kernel width at serial DAPs showed that V671 experienced an increased kernel width later than did Mc, but the rate and duration of increase were longer in V671. SDS-PAGE of the storage proteins and quantitative RT-PCR of cell cycle-related genes and indole-3-acetic (IAA) synthesis genes certified that the transition from mitosis to storage accumulation starts earlier in Mc. We hypothesized that the difference in the mitosis-to-storage accumulation transition accounts for the larger seed size in V671 vs. Mc.

Key words:

maize (Zea mays L.) , kernel , RNA-seq , mitosis-storage accumulation , transition , endosperm

. [J]. Journal of Integrative Agriculture, 2018, 17(07): 1574-1584.

ZHOU Yu-qian, WANG Qin-yang, ZHAO Hai-liang, GONG Dian-ming, SUN Chuan-long, REN Xue-mei, LIU Zhong-xiang, HE Hai-jun, QIU Fa-zhan. Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq[J]. Journal of Integrative Agriculture, 2018, 17(07): 1574-1584.

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Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq

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