Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

doi:10.1016/S2095-3119(17)61896-3

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (05): 1171-1180.DOI: 10.1016/S2095-3119(17)61896-3

收稿日期:2017-12-13 出版日期:2018-05-20 发布日期:2018-05-04

Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

ZHANG Zhi-bang*, XU Lei*, WEN Xue-xia, DONG Jian-guo, ZHOU Lei, GE Xin-na, YANG Han-chun, GUO Xin

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China

Received:2017-12-13 Online:2018-05-20 Published:2018-05-04
Contact: Correspondence YANG Han-chun, Tel/Fax: +86-10-62731296, E-mail: yanghanchun1 @cau.edu.cn; GUO Xin, Tel/Fax: +86-10-62732875, E-mail: guoxincau@cau.edu.cn
About author:ZHANG Zhi-bang, Tel: +86-10-62732875, E-mail: zhibangzhang @126.com; XU Lei, E-mail: 358736128@qq.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Technology R&D Program of China (2015BAD12B01-2), the Major Program of National Natural Science Foundation of China (31490603) and the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-36).

摘要/Abstract

Abstract: The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV) , nonstructural protein 10 (nsp10) , viral replication

. [J]. Journal of Integrative Agriculture, 2018, 17(05): 1171-1180.

ZHANG Zhi-bang, XU Lei, WEN Xue-xia, DONG Jian-guo, ZHOU Lei, GE Xin-na, YANG Han-chun, GUO Xin. Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells[J]. Journal of Integrative Agriculture, 2018, 17(05): 1171-1180.

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Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

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