GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean

doi:10.1016/S2095-3119(17)61721-0

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (03): 530-538.DOI: 10.1016/S2095-3119(17)61721-0

收稿日期:2017-02-24 出版日期:2018-03-20 发布日期:2018-03-01

GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean

LI Ming^{1, 2}, HU Zheng², JIANG Qi-yan², SUN Xian-jun², GUO Yuan², QI Jun-cang¹, ZHANG Hui²

¹ Agricultural College, Shihezi University, Shihezi 832003, P.R.China
² National Key Facilities for Crop Genetic Resources and Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2017-02-24 Online:2018-03-20 Published:2018-03-01
Contact: Correspondence QI Jun-cang,E-mail: qjc_agr@shzu.edu.cn; ZHANG Hui,E-mail:zhanghui06@caas.cn
About author:LI Ming,Tel/Fax:+86-10-62186654,E-mail:lmpapaya1987926@sina.com
Supported by:
This study was supported by the National Key Research and Development Program of China (2016YFD0101005) and the Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, Chinese Academy of Agricultural Sciences.

摘要/Abstract

Abstract: The NAC (NAM, ATAF1/2 and CUC2) transcription factor family plays a key role in plant development and responses to abiotic stress. GmNAC15 (Glyma15g40510.1), a member of the NAC transcription factor family in soybean, was functionally characterized, especially with regard to its role in salt tolerance. In the present study, qRT-PCR (quantitative reverse transcription PCR) analysis indicated that GmNAC15 was induced by salt, drought, low temperature stress, and ABA treatment in roots and leaves. GmNAC15 overexpression in soybean (Glycine max) hairy roots enhanced salt tolerance. Transgenic hairy roots improved the survival of wild leaves; however, overexpression of GmNAC15 in hairy root couldn’t influnce the expression level of GmNAC15 in leaf. GmNAC15 regulates the expression levels of genes responsive to salt stress. Altogether, these results provide experimental evidence of the positive effect of GmNAC15 on salt tolerance in soybean and the potential application of genetic manipulation to enhance the salt tolerance of important crops.

Key words: NAC , salt tolerance , soybean , hairy roots

. [J]. Journal of Integrative Agriculture, 2018, 17(03): 530-538.

LI Ming, HU Zheng, JIANG Qi-yan, SUN Xian-jun, GUO Yuan, QI Jun-cang, ZHANG Hui. GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean[J]. Journal of Integrative Agriculture, 2018, 17(03): 530-538.

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GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean

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