Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance

doi:10.1016/S2095-3119(16)61568-X

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (10): 2136-2144.DOI: 10.1016/S2095-3119(16)61568-X

收稿日期:2016-09-28 出版日期:2017-10-20 发布日期:2017-09-30

Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance

LIU Chang, YE Xing-guo, WANG Mei-jiao, LI Shi-jin, LIN Zhi-shan

National Key Facility of Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2016-09-28 Online:2017-10-20 Published:2017-09-30
Contact: Correspondence YE Xing-guo, Tel: +86-10-82109765, E-mail: yexingguo@caas.cn; LIN Zhi-shan, E-mail: linzhishan@caas.cn
Supported by:
This research was financially supported by the National Key Research and Development Program, China (2016YFD0102000) and the Agricultural Science and Technology Innovation Program (ASTIP) of the CAAS.

摘要/Abstract

Abstract: T6V#2S·6AL and T6V#4S·6DL translocation chromosomes developed from the cross of wheat and different Dasypyrum villosum accessions have good powdery mildew (PM) resistance, but their pairing and pyramiding behavior remains unclear. Results in this study indicated that the pairing frequency rate of the two differently originated 6VS chromosomes in their F₁ hybrid was 18.9% according to genomic in situ hybridization (GISH); the PM resistance plants in the F₂ generation from the cross between T6V#4S·6DL translocation line Pm97033 and its PM susceptible wheat variety Wan7107 was fewer than expected. However, the ratio of the resistant vs. the susceptible plants of 15:1 in the F₂ generation derived from the cross between the two translocation lines of T6V#2S·6AL and T6V#4S·6DL fitted well. Plants segregation ratio (homozygous:heterozygous:lacking) revealed by molecular marker for T6V#4S·6DL or T6V#2S·6AL in their F₂ populations fitted the expected values of 1:2:1 well, inferring that the pairing of the two alien chromosome arms facilitates the transmission of T6V#4S·6DL from the F₁ to the F₂ generation. A quadrivalent was also observed in 21% of pollen mother cells (PMCs) of homozygote plants containing the two pairs of translocated chromosomes. The chromosome pairing between 6V#2S and 6V#4S indicates that it will be possible to obtain recombinants and clarify if the PM resistance determinant on one alien chromosome arm is different from that on the other.

Key words: Triticum aestivum , Dasypyrum villosum , translocation , genetic behavior , powdery mildew resistance , GISH , molecular marker

. [J]. Journal of Integrative Agriculture, 2017, 16(10): 2136-2144.

LIU Chang, YE Xing-guo, WANG Mei-jiao, LI Shi-jin, LIN Zhi-shan. Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance[J]. Journal of Integrative Agriculture, 2017, 16(10): 2136-2144.

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Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance

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