Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization

doi:10.1016/S2095-3119(16)61477-6

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (09): 2110-2114.DOI: 10.1016/S2095-3119(16)61477-6

• 论文 • 上一篇

LIU Xue1, 2, ZHU Chang-xiong1, Frank Delvigne2, GONG Ming-bo3

收稿日期:2016-06-21 出版日期:2017-09-20 发布日期:2017-09-05

Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization

LIU Xue^{1, 2}, ZHU Chang-xiong¹, Frank Delvigne², GONG Ming-bo³

¹ Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
² Université de Liège, Gembloux Agro-Bio Tech, Unité de Bio-Industries, Gembloux 5030, Belgium
³ Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2016-06-21 Online:2017-09-20 Published:2017-09-05
Contact: Correspondence GONG Ming-bo, Tel: +86-10-82108683, E-mail: gongcaas@163.com
About author:LIU Xue, Tel: +86-10-82108683, E-mail: liuxue@caas.cn;
Supported by:
This work was supported by the National Natural Science Foundation of China (41440008).

摘要/Abstract

Abstract: A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5´ end of the RNA transcript (SMART) technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length cDNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame (ORF) of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family (BI-1-like) proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L⁻¹, which was associated with the release of 0.212 mg mL⁻¹ of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate (P).

Key words: phosphate solubilisation , Aspergillus niger , acetic acid

. [J]. Journal of Integrative Agriculture, 2017, 16(09): 2110-2114.

LIU Xue, ZHU Chang-xiong, Frank Delvigne, GONG Ming-bo. Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization[J]. Journal of Integrative Agriculture, 2017, 16(09): 2110-2114.

参考文献

Altomare C, Norvell A W, Bjorkman T, Harman G E. 1999. Solubilization of phosphates and micronutrients by the plant growth promoting and biocontrol fungus Trichoderma harzianum Fifai 1295-22. Applied and Environmental Microbiology, 65, 2926–2933.

Banik S, Dey B K. 1982. Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing micro-organisms. Plant and Soil, 69, 353–364.

Bianco C, Defez R. 2010. Improvement of phosphate solubilization and medicago plant yield by an indole-3-acetic acid-overproducing strain of Sinorhizobium meliloti. Applied and Environmental Microbiology, 76, 4626–4632.

Fomina M, Hillier S, Charnock J M, Melville K, Alexander I J, Gadd G M. 2004. Role of oxalic acid overexcretion in transformations of toxic metal minerals by Beauveria caledonica. Applied and Environmental Microbiology, 71, 371–381.

Goldstein A H, Braverman K, Osorio N. 1999. Evidence for mutualism between a plant growing in a phosphate-limited desert environment and a mineral phosphate solubilizing (MPS) rhizobacterium. FEMS Microbiology Ecology, 30, 295–300.

Gong M B, Du P, Liu X, Zhu C X. 2014b. An effective method for screening and testing the true phosphate-solubilizing fungus that enhances corn growth. Journal of Agricultural Sciences, 9, 60–70.

Gong M B, Tang C X, Zhu C X. 2014a. Cloning and expression of delta-1-pyrroline-5-carboxylate dehydrogenase in Escherichia coli DH5α improves phosphate solubilization. Canadian Journal of Microbiology, 60, 1–5.

Gulati A, Sharma N, Vyas P, Sood S, Rahi P, Pathania V, Prasad R. 2010. Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans-Himalayas. Archives of Microbiology, 192, 975–983.

Han K, Lim H, Hong J. 1992. Acetic acid formation in Escherichia coli fermentation. Biotechnology and Bioengineering, 39, 663–671.

Khan M S, Zaidi A,Wani P A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture - A review. Agronomy for Sustainable Development, 27, 29–43.

Lee K, Jung J, Kim k, Bae D, Lim D. 2009. Overexpression of outer membrane protein OprT and increase of membrane permeability in phoU mutant of toluene-tolerant bacterium Pseudomonas putida GM730. Journal of Microbiology, 47, 557–562.

Marchler-Bauer A, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, He S, Hurwitz D I, Jackson J D, Ke Z, Lanczycki C J, Liebert C A, Liu C, Lu F, Lu S, Marchler G H, et al. 2009. CDD: Specific functional annotation with the Conserved Domain Database. Nucleic Acids Research, 37, 205–210.

Marchler-Bauer A, Bryant S H. 2004. CD-Search: Protein domain annotations on the fly. Nucleic Acids Research, 32, 327–331.

Marchler-Bauer A, Derbyshire M K, Gonzales N R, Lu S, Chitsaz F, Geer L Y, Geer R C, He J, Gwadz M, Hurwitz D I, Lanczycki C J, Lu F, Marchler G H, Song J S, Thanki N, Wang Z, Yamashita R A, Zhang D, Zheng C, Bryant S H. 2015. CDD: NCBI’s conserved domain database. Nucleic Acids Research, 43, 222–226.

Marchler-Bauer A, Lu S, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, Hurwitz D I, Jackson J D, Ke Z, Lanczycki C J, Lu F, Marchler G H, Mullokandov M, Omelchenko M V, Robertson C L, et al. 2011. CDD: A Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Research, 39, 225–229.

Misra H S, Rajpurohit Y S, Khairnar N P. 2013. Pyrroloquinoline-quinone and its versatile roles in biological processes. Journal of Biosciences, 37, 313–325.

Nautiyal C S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 182, 265–270.

Walpola B C, Yoon M H. 2013. Phosphate solubilizing bacteria: Assessment of their effect on growth promotion and phosphorous uptake of mung bean (Vigna radiata [L.] R. Wilczek). Chilean Journal of Agricultural Research, 73, 275–281.

Werra P, Tarr M P, Keel C, Maurhofer M. 2009. Role of gluconic acid production in the regulation of biocontrol traits of Pseudomonas fluorescens CHA0. Applied and Environmental Microbiology, 75, 4162–4174.

Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics