Occurrence, identification and phylogenetic analyses of cereal cyst nematodes (Heterodera spp.) in Turkey

doi:10.1016/S2095-3119(16)61557-5

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (08): 1767-1776.DOI: 10.1016/S2095-3119(16)61557-5

收稿日期:2016-09-18 出版日期:2017-08-20 发布日期:2017-08-02

Occurrence, identification and phylogenetic analyses of cereal cyst nematodes (Heterodera spp.) in Turkey

CUI Jiang-kuan¹, PENG Huan¹, LIU Shi-ming¹, Gul Erginbas Orakci², HUANG Wen-kun¹, Mustafa Imren³, Abdelfattah Amer Dababat², PENG De-liang¹

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² CIMMYT (International Maize and Wheat Improvement Centre), Ankara 06511, Turkey
³ Department of Plant Protection, Faculty of Agriculture and Natural Science, Abant ?zzet Baysal University, Bolu 14280, Turkey

Received:2016-09-18 Online:2017-08-20 Published:2017-08-02
Contact: Correspondence Abdelfattah Amer Dababat, E-mail: a.dababat@cgiar.org; PENG De-liang, E-mail: pengdeliang@caas.cn
About author:CUI Jiang-kuan, E-mail: jk_cui@163.com;
Supported by:
This study was financially supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201503114 and 200903040) and the National Key Basic Research Program of China (973 Program, 2013CB127502). The authors would like to thank the Turkish Ministry of Food, Agriculture and Livestock; the International Wheat and Maize Improvement Center (CIMMYT, Mexico) for the help of sampling.

摘要/Abstract

Abstract: Plant-parasitic nematodes are very common on cereal crops and cause economic losses via reduction in grain quality and quantity. During 2014, 83 soil samples were collected from wheat and barley fields in 21 districts of 13 provinces across five regions (Central Anatolia, Marmara, Aegean, Southeast Anatolia, and Black Sea Region) of Turkey. Cyst-forming nematodes were found in 66 samples (80%), and the internal transcribed spacer (ITS) sequencing and species-specific PCR identified the species in 64 samples as Heterodera filipjevi, Heterodera latipons, and Heterodera avenae. The predominant pathogenic cereal cyst nematode was H. filipjevi, which was found in all five regions surveyed. H. avenae was only detected in Southeast Anatolia whereas H. latipons was detected in Southeast Anatolia and Central Anatolia. ITS-rDNA phylogenetic analyses showed that H. avenae isolates from China clustered with H. australis, and Turkish isolates were closely related to European and USA isolates of this species. H. filipjevi from Turkey and China were clustered closely with those from the UK, Germany, Russia, and the USA. The density of many of these populations exceeded or approached the maximum threshold level for economic loss. To our knowledge, this is the first report of H. filipjevi in Diyarbakir, Edirne, and Kutahya provinces, and the first report of H. avenae in Diyarbakir Province. These results exhibit the most rigorous analysis to date on the occurrence and distribution of Heterodera spp. in Turkey’s major wheat-producing areas, thus providing a basis for more specific resistance breeding, as well as other management practices.

Key words: species specific pcr , cereal cyst nematode , molecular identification , ITS-rDNA , wheat pathogen

. [J]. Journal of Integrative Agriculture, 2017, 16(08): 1767-1776.

CUI Jiang-kuan, PENG Huan, LIU Shi-ming, Gul Erginbas Orakci, HUANG Wen-kun, Mustafa Imren, Abdelfattah Amer Dababat, PENG De-liang . Occurrence, identification and phylogenetic analyses of cereal cyst nematodes (Heterodera spp.) in Turkey[J]. Journal of Integrative Agriculture, 2017, 16(08): 1767-1776.

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Occurrence, identification and phylogenetic analyses of cereal cyst nematodes (Heterodera spp.) in Turkey

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