The composition of Fusarium species in wheat husks and grains in south-eastern Poland

doi:10.1016/S2095-3119(16)61552-6

摘要/Abstract

Abstract: Fusarium populations were investigated on 53 samples of wheat grains and husks collected approximately three weeks before harvest in 53 wheat fields in south-eastern Poland. A limited area of sample collection was chosen intentionally to avoid the effect of climate and weather variability. The study was conducted to assess the occurrence of 13 Fusarium species using species-specific PCR assays separately on grains and husks of winter wheat. The obtained data suggest that husks could take a protective role of wheat grain against Fusarium spp. The incidence of Fusarium species decreased in grains vs. husks from 29 to 100%. While Fusarium species were present in husks at 11.32% and less, they were absent in the grain. The presence of Fusarium species on husks is inversely proportional to the percentage reduction of Fusarium spp. in grain. There was a correlation of the presence of certain species of Fusarium in husks and in grains. The number of Fusarium species found on husks was about three times higher in comparison to wheat grain. In conclusion, the presented data indicate the importance of Fusarium populations analysis on wheat husk in seeds pathological studies.

Key words: Fusarium spp. , wheat , husks , polymerase chain reaction

. [J]. Journal of Integrative Agriculture, 2017, 16(07): 1530-1536.

Adam Kuzdraliński, Micha? Nowak, Hubert Szczerba, Karolina Dudziak, Marta Muszyńska, Justyna Le?niowska-Nowak . The composition of Fusarium species in wheat husks and grains in south-eastern Poland[J]. Journal of Integrative Agriculture, 2017, 16(07): 1530-1536.

参考文献

Amatulli M T, Spadaro D, Gullino M L, Garibaldi A. 2010. Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity. Plant Pathology, 59, 839–844.

Aoki T, O’Donnell K. 1999. Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the group 1 population of

F. graminearum. Mycologia, 91, 597–609.

Balmas V, Scherm B, Marcello A, Beyer M, Hoffmann L, Migheli Q, Pasquali M. 2015. Fusarium species and chemotypes associated with Fusarium head blight and Fusarium root rot on wheat in Sardinia. Plant Pathology, 64, 972–979.

Bentley A R, Cromey M G, Farrokhi-Nejad R, Leslie J F, Summerell B A, Burgess L W. 2006. Fusarium crown and root rot pathogens associated with wheat and grass stem bases on the South Island of New Zeland. Australasian Plant Pathology, 35, 495–502.

Bottalico A, Perrone G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108, 611–624.

Buerstmayr H, Stierschneider M, Steiner B, Lemmens M, Griesser M, Nevo E. 2003. Variation for resistance to head blight caused by Fusarium graminearum in wild emmer (Triticum dicoccoides) originating from Israel. Euphytica, 130, 17–23.

Champeil A, Doré T, Fourbet J F. 2004. Fusarium head blight: Epidemiological origin of the effects of cultural practices on head blight attacks and the production of mycotoxins by Fusarium in wheat grains. Plant Science, 166, 1389–1415.

Czembor E, St?pień ?, Wa?kiewicz A. 2015. Effect of environmental factors on Fusarium species and associated mycotoxins in maize grain grown in Poland. PLoS ONE, 30, e0133644.

Demeke T, Clear R M, Patrick S K, Gaba D. 2005. Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. International Journal of Food Microbiology, 103, 271–284.

Faria C B, Abe C A, da Silva C N, Tessmann D J, Barbosa-Tessmann I P. 2012. New PCR assays for the identification of Fusarium verticillioides, Fusarium subglutinans and other species of the Gibberella fujikuroi complex. International Journal of Molecular Sciences, 13, 115–132.

Hope R, Aldred D, Magan N. 2005. Comparison of environmental profiles for growth and deoxynivalenol production by Fusarium culmorum and F. graminearum on wheat grain. Letters in Applied Microbiology, 40, 295–300.

Jurado M, Vazquez C, Patino B, Gonzalez-Jaen M T. 2005. PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Systematic and Applied Microbiology, 28, 562–568.

Kuzdraliński A, Szczerba H, Tofil K, Filipiak A, Garbarczyk E, Dziadko P, Muszyńska M, Solarska E. 2014. Early PCR-based detection of Fusarium culmorum, F. graminearum,

F. sporotrichioides and F. poae on stem bases of winter wheat throughout Poland. European Journal of Plant Pathology, 140, 491–502.

Liebert B. 2013. Create, collaborate and share advanced custom maps with Google Maps Engine Lite (Beta). Google Maps. [2016-01-01]. http://?google-latlong.?blogspot.?com/?2013/?03/?create-collaborate-and-share-advanced.? html

Mansfield M A, De Wolf E D, Kuldau G A. 2005. Relationships between weather conditions agronomic practices, and fermentation characteristics with deoxynivalenol content in fresh and ensiled maize. Plant Disease, 89, 1151–1157.

Marin S, Sanchis V, Ramos A J, Vinas I, Magan N. 1998. Environmental factors, in vitro interactions, and niche overlap between Fusarium moniliforme, F. proliferatum and F. graminearum, Aspergillus and Penicillium species from maize grain. Mycological Research, 102, 831–837.

Matny O. 2014. Fusarium head blight and crown rot on wheat & barley: Losses and health risks. Advances in Plants & Agriculture Research, 2, 00039.

Mishra P K, Fox R T V, Culham A. 2003. Development of PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiol Letters, 218, 329–332.

Möller E M, Che?kowski J, Geiger H H. 1999. Species-specific PCR assays for the fungal pathogens Fusarium moniliforme and Fusarium subglutinans and their application to diagnose maize ear rot disease. Journal of Phytopathology, 147, 497–508.

Nelson P E, Desjardins A E, Plattner R D. 1993. Fumonisins, mycotoxins produced by fusarium species: Biology, chemistry, and significance. Annual Review of Phytopathology, 31, 233–252.

Nelson P E, Dignani M C, Anaissie E J. 1994. Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews, 7, 479–504.

Nicholson P, Simpson D R, Weston G, Rezanoor H N, Lees A K, Parry D W, Joyce D. 1998. Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology, 53, 17–37.

O’Donnell K, Kistler H C, Cigelnik E, Ploetz R C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences of the United States of America, 95, 2044–2049.

Palazzini J M, Groenenboom-de Haas B H, Torres A M, Köhl J, Chulze S N. 2013. Biocontrol and population dynamics of Fusarium spp. on wheat stubble in Argentina. Plant Pathology, 62, 859–866.

Parry D W, Jenkinson P, McLeod L. 1995. Fusarium ear blight (scab) in small grain cereals - A review. Plant Patholology, 44, 207–238.

Parry D W, Nicholson P. 1996. Development of a PCR assay to detect Fusarium poae in wheat. Plant Patholology, 45, 383–391.

Perkowski J, Chelkowski J, Wakulinski W. 1990. Mycotoxins in cereal grain Part 13. Deoxynivalenol and 3-acetyl-deoxynivalenol in wheat kernels and chaff with head fusariosis symptoms. Molecular Nutrition & Food Research, 34, 325–328.

Reid L M, Nicol R W, Ouellet T, Savard M, Miller J D, Young J C, Stewart D W, Schaafsma A W. 1999. Interaction of Fusarium graminearum and F. moniliforme in maize ears: Sisease progress, fungal biomass, and mycotoxin accumulation. Phytopathology, 89, 1028–1037.

Simpson D R, Thomsett M A, Nicholson P. 2004. Competitive interactions between Microdochium nivale var. majus,

M. nivale var. nivale and Fusarium culmorum in planta and in vitro. Environmental Microbiology, 6, 79–87.

Smiley R W, Patterson L M. 1996. Pathogenic fungi associated with Fusarium foot rot of winter wheat in semiarid Pacific Northwest USA. Plant Disease, 80, 944–949.

Spanic V, Lemmens M, Drezner G. 2010. Morphological and molecular identification of Fusarium species associated with head blight on wheat in East Croatia. European Journal of Plant Pathology, 128, 511–516.

Suga H, Hirayama Y, Morishima M, Suzuki T, Kageyama K, Hyakumachi M. 2013. Development of PCR primers to identify Fusarium oxysporum f. sp. fragariae. Plant Disease, 97, 619–625.

Trenholm H L, Prelusky D B, Young J C, Miller J D. 1989. A practical guide to the prevention of Fusarium mycotoxins in grain and animal feedstuffs. Archives of Environmental Contamination and Toxicology, 18, 443–451.

Turner A S, Lees A K, Rezanoor H N, Nicholson P. 1998. Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology, 47, 278–288.

Vogelgsang S, Sulyok M. 2008. Toxigenicity and pathogenicity of Fusarium poae and Fusarium avenaceum on wheat. European Journal of Plant Pathology, 122, 265–276.

Wagacha J M, Muthomi J W. 2007. Fusarium culmorum: Infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop Protection, 26, 877–885.

Wang Y Z, Miller J D. 1988. Effects of fusarium graminearum metabolites on wheat tissue in relation to fusarium head blight resistance. Journal of Phytopathology, 122, 118–125.

Warfield C Y, Davis R M. 1996. Importance of the husk covering on the susceptibility of corn hybrids to Fusarium ear rot. Plant Disease, 80, 208–210.

Wegulo S N, Baenziger P S, Nopsa J H, Bockus W W, Hallen-Adams H. 2015. Management of Fusarium head blight of wheat and barley. Crop Protection, 73, 100–107.

Wilson A, Simpson D, Chandler E, Jennings P, Nicholson P. 2004. Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiology Letters, 233, 69–76.

Xu X, Madden L V, Edwards S G, Doohan F M, Moretti A, Hornok L, Nicholson P, Ritieni A. 2013. Developing logistic models to relate the accumulation of DON associated with Fusarium head blight to climatic conditions in Europe. European Journal of Plant Pathology, 137, 689–706.

Yoder W T, Christianson L M. 1998. Species-specific primers resolve members of Fusarium section Fusarium: Taxonomic status of the edible “Quorn” fungus reevaluated. Fungal Genetics and Biology, 23, 68–80.

Young J C, Miller J D. 1985. Appearance of fungus, ergosterol and Fusarium mycotoxins in the husk, axial stem, and stalk after inoculation of field corn. Canadian Journal of Plant Science, 65, 47–53.