Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato

doi:10.1016/S2095-3119(16)61553-8

摘要/Abstract

Abstract: Streptomyces rubrogriseus HDZ-9-47, isolated from eggs of Meloidogyne spp., was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field. Microscopic observations showed that HDZ-9-47 parasitized eggs of M. incognita within 7 days. In vitro, the culture filtrate of HDZ-9-47 caused 97.0% mortality of second-stage juveniles (J2s) of M. incognita and inhibited more than 50% egg hatching. In the field, compared with the control, the root-knot index and J2s density in the treatment of drench the broth contained 10¹² HDZ-9-47 spores were respectively reduced by 51.1 and 80.7% at 90 days post transplantation, which were better than that in other application doses and methods. In addition, reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%, respectively, better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment. And tomato yield also increased by 16.1%. Together, our results suggest that HDZ-9-47 could be an effective biocontrol agent of M. incognita, and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M. incognita control.

Key words: Meloidogyne incognita, Streptomyces rubrogriseus, biocontrol, parasitism, nematicidal metabolites, control efficacy

. [J]. Journal of Integrative Agriculture, 2017, 16(06): 1347-1357.

JIN Na, XUE Hui* LI Wen-jing, WANG Xue-yan, LIU Qian, LIU Shu-sen, LIU Pei, ZHAO Jian-long, JIAN Heng. Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato[J]. Journal of Integrative Agriculture, 2017, 16(06): 1347-1357.

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