Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanut×Arachis doigoi cross by cDNA-SCoT and HFO-TAG technique

doi:10.1016/S2095-3119(16)61462-4

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (05): 1093-1102.DOI: 10.1016/S2095-3119(16)61462-4

收稿日期:2016-05-06 出版日期:2017-05-20 发布日期:2017-05-08

Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanut×Arachis doigoi cross by cDNA-SCoT and HFO-TAG technique

HE Liang-qiong¹, TANG Rong-hua¹, JIANG Jing¹, XIONG Fa-qian¹, HUANG Zhi-peng¹, WU Hai-ning¹, GAO Zhong-kui¹, ZHONG Rui-chun¹, HE Xin-hua², HAN Zhu-qiang¹

¹Cash Crops Research Institute/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China
²Guangxi University, Nanning 530004, P.R.China

Received:2016-05-06 Online:2017-05-20 Published:2017-05-08
Contact: HE Xin-hua, Mobile: +86-15177169189, E-mail: honest66222@163.com; HAN Zhu-qiang, Mobile: +86-13977139289, E-mail: hanzhuqiang@163.com
About author:HE Liang-qiong, E-mail: heliangqiong@163.com
Supported by:
This project was supported by the Guangxi Academy of Agricultural Sciences Foundation, China (2015JZ08 and 2015YT57), the Guangxi Sciences Foundation, China (2011GXNSFA018079), the Modern Agro-industry Technology Research System, China (CARS-14-19) and the National Natural Science Foundation of China (31160294 and 31240059).

摘要/Abstract

Abstract: Allopolyploidy has played an important role in plant evolution and heterosis. Recent studies indicate that the process of wide hybridization and (or) polyploidization may induce rapid and extensive genetic and epigenetic changes in some plant species. To better understand the allopolyploidy evolutionism and the genetic mechanism of Arachis interspecific hybridization, this study was conducted to monitor the gene expression variation by cDNA start codon targeted polymorphism (cDNA-SCoT) and cDNA high-frequency oligonucleotide-targeting active gene (cDNA-HFO-TAG) techniques, from the hybrids (F₁) and newly synthesized allopolyploid generations (S₀-S₃) between tetraploid cultivated peanut Zhongkaihua 4 with diploid wild one Arachis doigoi. Rapid and considerable gene expression variations began as early as in the F₁hybrid or immediately after chromosome doubling. Three types of gene expression changes were observed, including complete silence (gene from progenitors was not expressed in all progenies), incomplete silence (gene expressed only in some progenies) and new genes activation. Those silent genes mainly involved in RNA transcription, metabolism, disease resistance, signal transduction and unknown functions. The activated genes with known function were almost retroelements by cDNA-SCoT technique and all metabolisms by cDNA-HFO-TAG. These findings indicated that interspecific hybridization and ploidy change affected gene expression via genetic and epigenetic alterations immediately upon allopolyploid formation, and some obtained transcripts derived fragments (TDFs) probably could be used in the research of molecular mechanism of Arachis allopolyploidization which contribute to thwe genetic diploidization of newly formed allopolyploids. Our research is valuable for understanding of peanut evolution and improving the utilization of putative and beneficial genes from the wild peanut.

Key words: peanut, allopolyploidy, gene expression, start codon-targeted polymorphism, high-frequency oligonucleotide-targeting active gene

. [J]. Journal of Integrative Agriculture, 2017, 16(05): 1093-1102.

HE Liang-qiong1, TANG Rong-hua1, JIANG Jing1, XIONG Fa-qian1, HUANG Zhi-peng1, WU Hai-ning1, GAO Zhong-kui1, ZHONG Rui-chun1, HE Xin-hua2, HAN Zhu-qiang1 . Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanut×Arachis doigoi cross by cDNA-SCoT and HFO-TAG technique[J]. Journal of Integrative Agriculture, 2017, 16(05): 1093-1102.

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Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanut×Arachis doigoi cross by cDNA-SCoT and HFO-TAG technique

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