Acute and subchronic toxicity as well as evaluation of safety pharmacology of modified pulsatilla granules

doi:10.1016/S2095-3119(16)61401-6

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (03): 671-678.DOI: 10.1016/S2095-3119(16)61401-6

收稿日期:2016-01-15 出版日期:2017-03-20 发布日期:2017-03-07

Acute and subchronic toxicity as well as evaluation of safety pharmacology of modified pulsatilla granules

JIA Rui-lin^1*, SONG Xu^1*, GUO Yu-fei², YIN Zhong-qiong¹, LIU Fei¹, XIONG Juan¹, LIU Qiu-yan¹, JIA Ren-yong¹, LI Li-xia¹, ZOU Yuan-feng¹, YIN Li-zi¹, HE Chang-liang¹, LIANG Xiao-xia¹, Yue Gui-zhou³

¹College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China
²Jinling Institute of Technology, Nanjing 211169, P.R.China
³College of Science, Sichuan Agricultural University, Ya’an 625014, P.R.China

Received:2016-01-15 Online:2017-03-20 Published:2017-03-07
Contact: JIA Ren-yong, E-mail: cqrc_jry@163.com; YIN Zhong-qiong, Tel: +86-835-2885614, E-mail: yinzhongq@163.com
About author:JIA Rui-lin, E-mail: 1032160689@qq.com
Supported by:
This study was supported by the National Natural Science Foundation of China (31372477), the International Cooperation Projects of Sichuan Province, China (2014HH0058, 2013HH0042), the Sichuan Youth Science and Technology Innovation Research Team for waterfowl disease prevention and control, China (2013TD0015).

摘要/Abstract

Abstract: The present study investigated acute and subchronic toxicity and safety pharmacology of modified pulsatilla granules (MPG) to provide a basis for a comprehensive understanding of MPG toxicity. The results of acute toxicity testing showed that the median lethal dose of MPG was more than 5 000 mg kg^–1, suggesting that MPG was considered as practically non-toxic. The subchronic toxicity study for 30 days was conducted by daily oral administration at doses of 375, 750 and 1 500 mg kg^–1 in Sprague-Dawley rats. The results of subchronic toxicity study showed that the body weight and relative organ weight were not significantly changed by administration of MPG. The clinical chemistry study showed that MPG could induce kidney and liver damages. In histopathological, mild lesions in liver and kidney were also observed, suggesting that the liver and kidney might be potential target organs of MPG. In the safety pharmacology study, MPG did not exhibited any side effects to rats in cardiovascular system, respiratory system and central nervous system. These results suggested that MPG could be considered safe for veterinary use.

Key words: modified pulsatilla granules, acute toxicity, subchronic toxicity, safety pharmacology

JIA Rui-lin, SONG Xu, GUO Yu-fei, YIN Zhong-qiong, LIU Fei, XIONG Juan, LIU Qiu-yan, JIA Ren-yong, LI Li-xia, ZOU Yuan-feng, YIN Li-zi, HE Chang-liang, LIANG Xiao-xia, YUE Gui-zhou . Acute and subchronic toxicity as well as evaluation of safety pharmacology of modified pulsatilla granules[J]. Journal of Integrative Agriculture, 2017, 16(03): 671-678.

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Acute and subchronic toxicity as well as evaluation of safety pharmacology of modified pulsatilla granules

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