Wnt gene expression in adult porcine longissimus dorsi and its association with muscle fiber type, energy metabolism, and meat quality

doi:10.1016/S2095-3119(16)61451-X

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (01): 144-150.DOI: 10.1016/S2095-3119(16)61451-X

收稿日期:2016-01-25 出版日期:2017-01-20 发布日期:2017-01-08

Wnt gene expression in adult porcine longissimus dorsi and its association with muscle fiber type, energy metabolism, and meat quality

MEN Xiao-ming, DENG Bo, TAO Xin, QI Ke-ke, XU Zi-wei

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, P.R.China

Received:2016-01-25 Online:2017-01-20 Published:2017-01-08
Contact: XU Zi-wei, Tel: +86-571-86404398, Fax: +86-571-86400481, E-mail: xzwfyz@sina.com
About author:MEN Xiao-ming, E-mail: menxiaoming@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31172220), the Modern Agro-industry Technology Research System of China (CARS-36), and the Science and Technology Projects in Zhejiang Province of China (LY17C170005, 2016C02054-4).

摘要/Abstract

Abstract: This study investigated the expression profiles of Wnt genes in adult porcine longissimus dorsi (LD) from different porcine genotypes and their associations with meat quality. The results showed that Wnt5a gene expression level was the highest in Jinhua (JHP) pigs, followed by Zhongbai (ZBP), Duroc×Zhongbai (DZB), and Duroc×Yorkshire×Landrace (DYL) pigs, with significant differences between ZBP, DZB, and DYL (P<0.05). This genotypic order was reversed for Wnt7a, Wnt10b, and Wnt11 expression, with JHP and DYL having the lowest and highest expressive levels, respectively. Wnt5a expression was negatively correlated with pH_{45 min} and ΔpH (P<0.01), some glycolytic markers (P<0.05), and positively correlated with meat color (a*), shear force (SF) value (P<0.05), myosion heavy chain (MyHC) I mRNA proportion (P<0.01), turnover ratio of creatine phosphate (CP), and creatine kinase (CK) activity (P<0.05). Opposite correlations were observed for Wnt2, Wnt7a, Wnt10b, and Wnt11. These results revealed that Wnt5a, Wnt7a, Wnt10b, and Wnt11 gene expressions in adult porcine muscle contributed to differences between porcine genotypes and affected pork quality. Wnt5a gene expression could be beneficial for the formation of high quality pork by regulating muscle fiber types and postmortem energy metabolism

Key words: pigs, meat quality, Wnt genes, muscle-fiber type, energy metabolism

MEN Xiao-ming, DENG Bo, TAO Xin, QI Ke-ke, XU Zi-wei. [J]. Journal of Integrative Agriculture, 2017, 16(01): 144-150.

MEN Xiao-ming, DENG Bo, TAO Xin, QI Ke-ke, XU Zi-wei. Wnt gene expression in adult porcine longissimus dorsi and its association with muscle fiber type, energy metabolism, and meat quality[J]. Journal of Integrative Agriculture, 2017, 16(01): 144-150.

参考文献

Anakwe K, Robson L, Hadley J, Buxton P, Church V, Allen S, Hartmann C, Harfe B, Nohno T, Brown A M, Evans D J, Francis-West P. 2003. Wnt signalling regulates myogenic differentiation in the developing avian wing. Development, 130, 3503–3514.

Bonneau M, Lebret B. 2010. Production systems and influence on eating quality of pork. Meat Science, 84, 293–300.

Brack A S, Conboy I M, Conboy M J, Shen J, Rando T A. 2008. A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis. Cell Stem Cell, 2, 50–59.

Brack A S, Conboy M J, Roy S, Lee M, Kuo C J, Keller C, Rando T A. 2007. Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis. Science, 317, 807–810.

Brunelli S, Relaix F, Baesso S, Buckingham M, Cossu G. 2007. Beta catenin-independent activation of MyoD in presomitic mesoderm requires PKC and depends on Pax3 transcriptional activity. Developmental Biology, 304, 604–614.

Chung S S, Lee J S, Kim M, Ahn B Y, Jung H S, Lee H M, Kim J W, Park K S. 2012. Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis. Obesity, 20, 482–487.

Damon M, Denieul K, Vincent A, Bonhomme N, Wyszynska-Koko J, Lebret B. 2013. Associations between muscle gene expression pattern and technological and sensory meat traits highlight new biomarkers for pork quality assessment. Meat Science, 95, 744–754.

De A. 2011. Wnt/Ca2+ signaling pathway: A brief overview. Acta BIochimical et BIophysica Sinica, 43, 745–756.

Gros J, Serralbo O, Marcelle C. 2009. Wnt11 acts as a directional cue to organize the elongation of early muscle fibres. Nature, 457, 589–593.

Han X H, Jin Y R, Seto M, Yoon J K. 2011. A WNT/beta-catenin signaling activator, R-spondin, plays positive regulatory roles during skeletal myogenesis. Journal of Biological Chemistry, 286, 10649–10659.

He X P, Gao H, Liu C X, Fan B, Liu B. 2011. Cloning, chromosomal localization, expression profile and association analysis of the porcine Wnt10b gene with backfat thickness. Molecular Biology Reports, 38, 3095–3099.

Hutcheson D A, Zhao J, Merrell A, Haldar M, Kardon G. 2009. Embryonic and fetal limb myogenic cells are derived from developmentally distinct progenitors and have different requirements for beta-catenin. Genes and Development, 23, 997–1013.

Kang S, Bennett C N, Gerin I, Rapp L A, Hankenson K D, Macdougald O A. 2007. Wnt signaling stimulates osteoblastogenesis of mesenchymal precursors by suppressing CCAAT/enhancer binding protein alpha and peroxisome proliferator-activated receptor gamma. Journal of Biology Chemistry, 282, 14515–14524.

Kikuchi A, Yamamoto H, Sato A. 2009. Selective activation mechanisms of Wnt signaling pathways. Trends Cell Biology, 19, 119–129.

Kim C H, Neiswender H, Baik E J, Xiong W C, Mei L. 2008. β-Catenin interacts with MyoD and regulates its transcription activity. Molecular and Cellular Biochemistry, 28, 2941–2951.

Lefaucheur L. 2010. A second look into fiber typing - Relation to meat quality. Meat Science, 84, 257–270.

Lisa M, Andrew J W, Biswajit P, Cao Y, Tyler A, Moens C B, Tapscott S J. 2007. Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation. Development, 134, 3371–3382.

Luo X, Li H X, Yang G S. 2008. Sequential expression of Wnt/β-catenin signal pathway related genes and adipocyte transcription factors during porcine adipose tissue development. Chinese Journal of Biotechnology, 24, 746–753. (in Chinese)

Maltzahn J V, Chang N C, Bentzinger C F, Rudnicki M A. 2012. Wnt signaling in myogenesis. Trends in Cell Biology, 22, 602–609.

Men X M, Deng B, Xu Z W, Liu M H, Qi K K. 2011. Characteristics of ATP-CP system status in postmortem muscle and their associations with pork quality traits. Scientia Agricultura Sinca, 44, 1457–1465. (in Chinese)

Men X M, Deng B, Xu Z W, Tao X. 2012. Muscle-fibre types in porcine longissimus muscle of different genotypes and their association with the status of energy metabolism. Animal Production Science, 52, 305–312.

Mu H P, Lin S M, Ye Y Q, Zhang X Q, Li Y G. 2012. Down-regulation of Wnt-10a/catenin-Β and TGF-Β 3 promotes fat deposition dwarf chicken muscle. In: Animal Anatomy and Embryology Branch 17th Research Association of Chinese Association of Animal Science and Veterinary Medicine. July 10th–13th, Taigu, China. pp. 355–366. (in Chinese)

Newman D, Magolski J. 2014. Quality Management/Farm Level: Pork Quality. Encyclopedia of Meat Sciences. 2nd ed. Elsevier Science, Amsterdam. pp. 168–172.

Scarda A, Franzin C, Milan G, Sanna M, Dal Prà C, Pagano C, Boldrin L, Piccoli M, Trevellin E, Granzotto M, Gamba P, Federspil G, De Coppi P, Vettor R. 2010. Increased adipogenic conversion of muscle satellite cells in obese Zucker rats. International Journal of Obesity, 34, 1319–1327.

Scheffler T L, Park S, Gerrard D E. 2011. Lessons to learn about postmortem metabolism using the AMPKγ3R200Q mutation in the pig. Meat Science, 89, 244–250.

Shi X E, Liu Y G, Yang Q M, Chen Z Z, Yang G S. 2012. Role of wnt/β-catenin in the differentiation of satellite cells into muscle fibers. Scientia Agricultura Sinca, 45, 1380–1386. (in Chinese)

Wodarz A, Nusse R. 1998. Mechanisms of Wnt signaling in development. Annual Review of Cell and Developmental Biology, 14, 59–88.

Xu L H, Liu C L, Chang Y M, Liang L Q, Liu J L, Gao G Q, Han Q X. 2011. Theory and method of double-standard curves method of relative quantification PCR. Biotechnology Bulletin, 1, 70–75. (in Chinese)

Yang Q M, Liu Y G, Shen Q W, Shi X E, Yang G S. 2012. Activation canonical Wnt signaling by LiCi induces porcine skeletal muscle satellite cells differentiation into slow muscle. Chinese Journal of Animal Science, 48, 21–27. (in Chinese)

Zeve D, Seo J, Suh J M, Seo J, Suh J M, Stenesen D, Tang W, Berglund E D, Wan Y, Williams L J, Lim A, Martinez M J, McKay R M, Millay D P, Olson E N, Graff J M. 2012. Wnt signaling activation in adipose progenitors promotes insulin-independent muscle glucose uptake. Cell Metabolism, 15, 492–504.

Zhai H Q, Shen G Y, Wang P Z, Xi C L, Li N, Wang T Y, Feng X H, Chen R A, Zhou Z G, Gu X H, Wan X Q, E Y X, Shi S Y, Fan Z X, Wang B X. 2014. CAS 235–2014, Farm Animal Welfare Requirements: Pigs. Standards of China Association, Beijing. (in Chinese)

Zhou D, Strakovsky R S, Zhang X Y, Pan Y X. 2012. The skeletal muscle Wnt pathway may modulate insulin resistance and muscle development in a diet-induced obese rat model. Obesity, 20, 1577–1584.

Zhou G H, Zhao G M. 2007. Biochemical changes during processing of traditional Jinhua ham. Meat Science, 77, 114–120.

Zhou Y M, Deng W B. 2009. SPSS 16.0 and Statistical Data Analysis. Southwest University of Finance and Economics, Chengdu. (in Chinese)

Zou H, Li R, Jia Y, Yang X, Ni Y, Cong R, Soloway P D, Zhao R. 2012. Breed-dependent transcriptional regulation of 5´-untranslated GR (NR3C1) exon 1 mRNA variants in the liverof newborn piglets. PLoS ONE, 7, e40432.

Wnt gene expression in adult porcine longissimus dorsi and its association with muscle fiber type, energy metabolism, and meat quality

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics