Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China

doi:10.1016/S2095-3119(16)61452-1

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (01): 125-134.DOI: 10.1016/S2095-3119(16)61452-1

收稿日期:2016-03-25 出版日期:2017-01-20 发布日期:2017-01-08

Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China

YUAN Guo-hui¹, GUO Wen-lei¹, DU Long¹, LIU Wei-tang¹, LI Qi¹, LI Ling-xu², WANG Jin-xin¹

¹College of Plant Protection, Shandong Agricultural University, Tai’an 271018, P.R.China
²College of Chemistry and Pharmacy Science, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2016-03-25 Online:2017-01-20 Published:2017-01-08
Contact: WANG Jin-xin, Tel: +86-538-8241114, E-mail: wangjx@sdau.edu.cn
About author:YUAN Guo-hui, E-mail: mypostok@126.com
Supported by:
This research was financially supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201303031) and the National Natural Science Foundation of China (31471787).

摘要/Abstract

Abstract: Keng stiffgrass is a grass weed that affects wheat-rice cropping systems in China. The extensive reliance on acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides has resulted in keng stiffgrass developing resistance to these herbicides. The objective of this research was to evaluate the resistance level of the putative resistant keng stiffgrass populations to ACCase-inhibiting herbicides and to identify their molecular resistance mechanism. Whole-plant dose-response experiments demonstrated that SD-4 (R), SD-11 (R), and JS-25 (R) populations were highly resistant to fenoxaprop, clodinafop, and fluazifop, moderately resistant to diclofop, had low resistance to sethoxydim and pinoxaden, but were sensitive to clethodim. Partial chloroplastic ACCase sequences showed that there were two copies of ACCase gene in keng stiffgrass, and all homoeologous genes were expressed. The results of sequence analyses of the ACCase CT domain revealed an isoleucine-to-asparagine substitution at position 2041 in SD-4 (R) and SD-11 (R) populations, and a tryptophan-to-cysteine substitution at position 2027 in the JS-25 (R) population. To our knowledge, this is the first report of Ile-2041-Asn and Trp-2027-Cys mutations in ACCase-resistant keng stiffgrass. In addition, three robust (derived) cleaved amplified polymorphic sequence ((d)CAPS) markers have been developed to rapidly identify these mutations in the ACCase gene of keng stiffgrass.

Key words: herbicide resistance, ACCase gene, mutation, (d)CAPS

YUAN Guo-hui, GUO Wen-lei, DU Long, LIU Wei-tang, LI Qi, LI Ling-xu, WANG Jin-xin. [J]. Journal of Integrative Agriculture, 2017, 16(01): 125-134.

YUAN Guo-hui, GUO Wen-lei, DU Long, LIU Wei-tang, LI Qi, LI Ling-xu, WANG Jin-xin. Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China[J]. Journal of Integrative Agriculture, 2017, 16(01): 125-134.

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Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China

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