Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (10): 2183-2191.DOI: 10.1016/S2095-3119(15)61190-X

• 论文 •    下一篇

  

  • 收稿日期:2015-09-07 出版日期:2016-10-01 发布日期:2016-10-01

Experimental and genomic evidence for the indica-type cytoplasmic effect in Oryza sativa L. ssp. japonica

LIU You-hong*, TANG Liang*, XU Quan*, MA Dian-rong, ZHAO Ming-hui, SUN Jian, CHEN Wen-fu   

  1. Rice Research Institute, Shenyang Agricultural University/Key Laboratory of Northern japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province/Key Laboratory of Northeast Rice Biology and Genetics and Breeding, Ministry of Agriculture/Collaborative Innovation Center of japonica Rice Genetic Improvement and Production in Northeast China, Shenyang 110866, P.R.China
  • Received:2015-09-07 Online:2016-10-01 Published:2016-10-01
  • Contact: SUN Jian, E-mail: as_1230@163.com; CHEN Wen-fu, Tel/Fax: +86-24-88487184, E-mail: wfchen5512@126.com
  • About author:LIU You-hong, E-mail: liuyouhong1011@126.com;
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31371587 and 31430062), the Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province (2014046), the China Postdoctoral Science Foundation Grant (2014M560221) and the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT), China.

Abstract:     Cytoplasmic effects are important agronomical phenomena that have generated widespread interest in both theory and application. In the present study, five high yield rice cultivars (Oryza sativa L. ssp. japonica) in large-scale cultivation in northeast China were determined to possess Oryza sativa L. ssp. indica-type cytoplasm using cytoplasmic subspecies-specific molecular markers. This was confirmed by cytoplasmic genome-wide single nucleotide polymorphisms (SNPs) and functional gene sequencing. Two of these five japonica cultivars were core breeding parents with high yield and the other three were super-high-yield varieties registered by the Ministry of Agriculture of China. We constructed nuclear substitution lines to further demonstrate whether and how this indica-type cytoplasm contributed to yield improvement by comparing yield components. The results showed that under the same japonica nuclear background, the lines with indica-type cytoplasm had a significant decrease in tillers in exchange for increased grain number per panicle compared with their recurrent parents. Our results implied that botanical basis of this cytoplasmic effect was to reduce the plant’s branching differentiation to produce more floral organs under the constant nutrition. Our findings open another door for the utilization of inter-subspecific hybridization for the improvement of rice cultivar.

Key words: cytoplasmic effects ,  cytoplasmic genome-wide SNPs ,  super high yield rice ,  nuclear substitution lines