QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)

doi:10.1016/S2095-3119(15)61259-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (8): 1693-1702.DOI: 10.1016/S2095-3119(15)61259-X

QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)

收稿日期:2015-07-29 出版日期:2016-08-01 发布日期:2016-08-01

QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)

GAO Fang-yuan^1*, ZENG Li-hua^2*, QIU Ling^{1, 2}, LU Xian-jun¹, REN Juan-sheng¹, WU Xian-ting¹, SU Xiang-wen¹, GAO Yong-ming³, REN Guang-jun¹

¹Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
² Sichuan Normal University, Chengdu 610110, P.R.China
³ Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2015-07-29 Online:2016-08-01 Published:2016-08-01
Contact: REN Guang-jun, Tel: +86-28-84504006, Fax: +86-28-84790147, E-mail: guangjun61@sina.com
About author:GAO Fang-yuan, Mobile: +86-13618022481, Tel: +86-28-84504558, E-mail: gfy246@163.com; ZENG Li-hua, Tel: +86-28-84788266, E-mail: zenglihua66@hotmail.com;
Supported by:
This work was supported by a grant from the National High-Tech R&D Program of China (2014AA10A603, 2014AA10A604), a grant from the Youth Foundation in Sichuan, China (2011JTD0022), the special fund for China Agricultural Research System (CARS-01-08) and the Provincial Specialized Funds for Innovation Ability Promotion in Sichuan, China (2013GXJS005).

摘要/Abstract

Abstract: Grain appearance quality traits, measured as grain length (GL), grain width (GW), length to width ratio (LWR), grain thickness (GT) and the percentage of grain with chalkiness (PGWC), as well as 1 000-grain weight (TGW), are very important factors that contribute to rice grain quality and yield. To detect quantitative trait loci (QTLs) affecting these traits, we developed a set of recombinant inbred lines (RILs) derived from Gang46B (G46B) and K1075, a G46B introgression line with lower PGWC. Based on a linkage map containing 33 simple sequence repeat (SSR) markers, a total of 15 additive QTLs governing six measured traits were identified on 4 chromosomes across two environments. Of these, the five major QTLs which controlled GW, LWR, GT, PGWC, and TGW, each explaining up to 44.30, 55.29, 62.30, 30.94, and 28.78% of the variation, respectively, were found in the same interval of RM18004–RM18068 on chromosome 5. The G46B alleles contributed to the increase in GW, GT and PGWC at all loci, as well as the increase in TGW at its major QTL locus. Significant interactions between additive QTL and the environment were found at most loci, in which the largest, accounting for 15.06% of variation, was observed between qPGWC-5 and the environment. A total of 15 epistasis QTLs were detected for all the traits, and GL, GW and PGWC had significant epistasis QTLs based on environment interactions with minor effects. These results are valuable for future map-based cloning of the QTLs and the collaborative improvement of G46B in grain appearance quality and yield.

Key words: QTL , appearance quality , 1 000-grain weight , recombination inbred lines (RIL) , rice

GAO Fang-yuan, ZENG Li-hua, QIU Ling, LU Xian-jun, REN Juan-sheng, WU Xian-ting, SU Xiangwen, GAO Yong-ming, REN Guang-jun. QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2016, 15(8): 1693-1702.

GAO Fang-yuan, ZENG Li-hua, QIU Ling, LU Xian-jun, REN Juan-sheng, WU Xian-ting, SU Xiangwen, GAO Yong-ming, REN Guang-jun. QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2016, 15(8): 1693-1702.

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QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)

QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)

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