Proteome analysis of alfalfa roots in response to water deficit stress

doi:10.1016/S2095-3119(15)61255-2

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (06): 1275-1285.DOI: 10.1016/S2095-3119(15)61255-2

Proteome analysis of alfalfa roots in response to water deficit stress

收稿日期:2015-05-14 出版日期:2016-06-01 发布日期:2016-06-06

Proteome analysis of alfalfa roots in response to water deficit stress

Rahman Md Atikur¹, Kim Yong-Goo¹, Alam Iftekhar^{1, 3}, LIU Gong-she², Lee Hyoshin⁴, Lee Jeung Joo⁵, Lee Byung-Hyun¹

¹Division of Applied Life Sciences (BK21Plus), Institute of Agriculture & Life Sciences (IALS), Gyeongsang National University, Jinju 660-701, Republic of Korea
² Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China
³ National Institute of Biotechnology, Dhaka-1349, Bangladesh
⁴ Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 441-847, Republic of Korea
⁵ Department of Applied Biology, Institute of Agriculture & Life Sciences (IALS), Gyeongsang National University, Jinju 660-701, Republic of Korea

Received:2015-05-14 Online:2016-06-01 Published:2016-06-06
Contact: Lee Byung-Hyun, Tel: +82-55-7721882, Fax: +82-55-7721889, E-mail: hyun@gnu.ac.kr
About author:Rahman Md Atikur, E-mail: atikgnu@gmail.com
Supported by:
This work was supported by the National Research Foundation of Korea (NRF) Grant (NRF-2011-616-F00013). Alam I was supported by post-doctoral grant, and Rahman M A, Kim Y-G were supported by the scholarship from BK21Plus program, Ministry of Education, Republic of Korea.

摘要/Abstract

Abstract: To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H₂O₂ content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.

Key words: alfalfa , proteome , root , water deficit stress

Rahman Md Atikur, Kim Yong-Goo, Alam Iftekhar, LIU Gong-she, Lee Hyoshin, Lee Jeung Joo, Lee Byung-Hyun. Proteome analysis of alfalfa roots in response to water deficit stress[J]. Journal of Integrative Agriculture, 2016, 15(06): 1275-1285.

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Proteome analysis of alfalfa roots in response to water deficit stress

Proteome analysis of alfalfa roots in response to water deficit stress

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