Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

doi:10.1016/S2095-3119(15)61283-7

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (05): 973-982.DOI: 10.1016/S2095-3119(15)61283-7

Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

CHEN Ji-bao¹, CAO Yuan-nan¹, ZHANG Zhao-yuan¹, WANG Shu-min², WU Jing², WANG Lan-fen²

¹ School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.China
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2016-04-07 出版日期:2016-05-03 发布日期:2016-05-03
通讯作者: WANG Shu-min, Tel: +86-10-62175628, E-mail: shuminwang@caas.cn
作者简介:CHEN Ji-bao, E-mail: chenjibao2012@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (31471559) and the Higher Education Institution Key Research Project Plan of Henan Province, China (15A210042).

Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

CHEN Ji-bao¹, CAO Yuan-nan¹, ZHANG Zhao-yuan¹, WANG Shu-min², WU Jing², WANG Lan-fen²

¹ School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.China
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2016-04-07 Online:2016-05-03 Published:2016-05-03
Contact: WANG Shu-min, Tel: +86-10-62175628, E-mail: shuminwang@caas.cn
About author:CHEN Ji-bao, E-mail: chenjibao2012@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31471559) and the Higher Education Institution Key Research Project Plan of Henan Province, China (15A210042).

摘要/Abstract

Abstract: Drought stress is a major abiotic stress of common bean (Phaseolus vulgaris L.) throughout the world. Increasing the proline accumulation contributes to enhance crop drought tolerance. A cDNA for δ-ornithine aminotransferase (δ-OAT), an enzyme involved in the biosynthesis of proline, was isolated from Phaseolus vulgaris (PvOAT). PvOAT exhibits 87.4 and 39.8% similarity of the deduced amino acid sequences with δ-OAT from Glycine max and Vigna aconitifolia, respectively. The transcriptional analysis revealed that PvOAT was strongly induced by drought stress. And the expression of PvOAT was higher in leaves than that in the root and stem of common bean by drought stress. Similar increase of the proline accumulation was observed in leaves and roots of common bean by drought stress. Furthermore, the proline content, the PvOAT expression and the PvOAT enzyme activity in cultivar F5575 was significantly (P<0.01) higher than that in cultivar F4851 under drought-stress conditions. Interestingly, it had been observed that, in the later stage of drought stress, the proline steadily maintained at the maximum level maybe result from the PvOAT enzyme activity increasing steadily. These results indicated that the expression of PvOAT and the accumulation of proline induced by drought stress treatment were related to the degree of common bean drought tolerance. So our results support the view that δ-OAT is associated with proline synthesis under drought stress conditions.

Key words: common bean , ornithine aminotransferase gene , proline , drought tolerance

CHEN Ji-bao, CAO Yuan-nan, ZHANG Zhao-yuan, WANG Shu-min, WU Jing, WANG Lan-fen. Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.[J]. Journal of Integrative Agriculture, 2016, 15(05): 973-982.

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Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

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