Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (05): 944-953.DOI: 10.1016/S2095-3119(15)61310-7

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YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development

WANG Zhong-wei*, ZHANG Tian-quan*, XING Ya-di, ZENG Xiao-qin, WANG Ling, LIU Zhong-xian, SHI Jun-qiong, ZHU Xiao-yan, MA Ling, LI Yun-feng, LING Ying-hua, SANG Xian-chun, HE Guang-hua   

  1. Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.China
  • 收稿日期:2015-12-07 出版日期:2016-05-03 发布日期:2016-05-03
  • 通讯作者: HE Guang-hua, Tel: +86-23-68250158, E-mail: heghswu@163.com
  • 作者简介:* These authors contributed equally to this study.
  • 基金资助:

    This research was supported by the Special Fund for Industry of Ministry of Agriculture of China (201303129), the Fundamental Research Funds for the Central Universities, China (XDJK2013A023), the Key Program of Chongqing, China (cstc2012ggC80002), and the Upgrade Project of the Key Laboratory of Chongqing, China (cstc2014pt-sy80001).

YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development

WANG Zhong-wei*, ZHANG Tian-quan*, XING Ya-di, ZENG Xiao-qin, WANG Ling, LIU Zhong-xian, SHI Jun-qiong, ZHU Xiao-yan, MA Ling, LI Yun-feng, LING Ying-hua, SANG Xian-chun, HE Guang-hua   

  1. Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.China
  • Received:2015-12-07 Online:2016-05-03 Published:2016-05-03
  • Contact: HE Guang-hua, Tel: +86-23-68250158, E-mail: heghswu@163.com
  • About author:* These authors contributed equally to this study.
  • Supported by:

    This research was supported by the Special Fund for Industry of Ministry of Agriculture of China (201303129), the Fundamental Research Funds for the Central Universities, China (XDJK2013A023), the Key Program of Chongqing, China (cstc2012ggC80002), and the Upgrade Project of the Key Laboratory of Chongqing, China (cstc2014pt-sy80001).

Abstract:     The nuclear-encoded light-harvesting chlorophyll a/b-binding proteins (LHCPs) are specifically translocated from the stroma into the thylakoid membrane through the chloroplast signal recognition particle (cpSRP) pathway. The cpSRP is composed of a cpSRP43 protein and a cpSRP54 protein, and it forms a soluble transit complex with LHCP in the chloroplast stroma. Here, we identified the YGL9 gene that is predicted to encode the probable rice cpSRP43 protein from a rice yellow-green leaf mutant. A phylogenetic tree showed that an important conserved protein family, cpSRP43, is present in almost all green photosynthetic organisms such as higher plants and green algae. Sequence analysis showed that YGL9 comprises a chloroplast transit peptide, three chromodomains and four ankyrin repeats, and the chromodomains and ankyrin repeats are probably involved in protein-protein interactions. Subcellular localization showed that YGL9 is localized in the chloroplast. Expression pattern analysis indicated that YGL9 is mainly expressed in green leaf sheaths and leaves. Quantitative real-time PCR analysis showed that the expression levels of genes associated with pigment metabolism, chloroplast development and photosynthesis were distinctly affected in the ygl9 mutant. These results indicated that YGL9 is possibly involved in pigment metabolism, chloroplast development and photosynthesis in rice.

Key words: Oryza sativa , yellow-green leaf gene ,  cpSRP43 ,  light-harvesting chlorophyll a/b-binding protein