High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

doi:10.1016/S2095-3119(15)61104-2

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (4): 862-871.DOI: 10.1016/S2095-3119(15)61104-2

High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

HAN Ji-long¹, YANG Min², GUO Ting-ting¹, LIU Jian-bin¹, NIU Chun-e¹, YUAN Chao¹, YUE Yao-jing¹, YANG Bo-hui¹

¹ Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China
² Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

收稿日期:2015-03-09 出版日期:2016-04-01 发布日期:2016-04-07
通讯作者: YUE Yao-jing, E-mail: yueyaojing@126.com; YANG Bo-hui, E-mail: yangbh2004@163.com
作者简介:HAN Ji-long, E-mail: hanjilong10000@126.com
基金资助:
This work was financially sponsored by the Earmarked Fund for Modern China Wool & Cashmere Technology Research System (CARS-40-03) and the National Natural Science Foundation for Young Scholars of China (31402057). Project support was provided by the ASTIP (Agricultural Science and Technology Innovation Program) for Genetic Resource and Breeding of Fine-Wool Sheep, Chinese Academy of Agricultural Sciences.

High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

HAN Ji-long¹, YANG Min², GUO Ting-ting¹, LIU Jian-bin¹, NIU Chun-e¹, YUAN Chao¹, YUE Yao-jing¹, YANG Bo-hui¹

¹ Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China
² Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Received:2015-03-09 Online:2016-04-01 Published:2016-04-07
Contact: YUE Yao-jing, E-mail: yueyaojing@126.com; YANG Bo-hui, E-mail: yangbh2004@163.com
About author:HAN Ji-long, E-mail: hanjilong10000@126.com
Supported by:
This work was financially sponsored by the Earmarked Fund for Modern China Wool & Cashmere Technology Research System (CARS-40-03) and the National Natural Science Foundation for Young Scholars of China (31402057). Project support was provided by the ASTIP (Agricultural Science and Technology Innovation Program) for Genetic Resource and Breeding of Fine-Wool Sheep, Chinese Academy of Agricultural Sciences.

摘要/Abstract

摘要： The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain (Xinjiang types) (CMS) and 5 loci in Gansu alpine superfine-wool sheep strain (GSS) groups were found deviated from Hardy-Weinberg equilibrium (HWE). Genetic drift F_ST=0.019 (P<0.001) and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep (LS) and Hu sheep (HY) reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.

关键词: Chinese fine-wool sheep , indigenous sheep breeds , genetic relationship , gene flow , microsatellites

Abstract: The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain (Xinjiang types) (CMS) and 5 loci in Gansu alpine superfine-wool sheep strain (GSS) groups were found deviated from Hardy-Weinberg equilibrium (HWE). Genetic drift F_ST=0.019 (P<0.001) and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep (LS) and Hu sheep (HY) reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.

Key words: Chinese fine-wool sheep , indigenous sheep breeds , genetic relationship , gene flow , microsatellites

HAN Ji-long, YANG Min, GUO Ting-ting, LIU Jian-bin, NIU Chun-e, YUAN Chao, YUE Yao-jing, YANG Bo-hui . High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China[J]. Journal of Integrative Agriculture, 2016, 15(4): 862-871.

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High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

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