Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (3): 650-657.DOI: 10.1016/S2095-3119(14)60971-0

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Effect of Fuzheng Jiedu granule on immunological function and level of immune-related cytokines in immune-suppressed mice

 YU Zhan-mei, HUANG Xian-hui, YAN Chao-qun, GAO Jin, LIANG Zi-sen   

  1. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China
  • 收稿日期:2015-01-08 出版日期:2016-03-07 发布日期:2016-03-09
  • 通讯作者: HUANG Xian-hui, Mobile: +86-13808842259,Fax: +86-20-87344801, E-mail: xhhuang@scau.edu.cn;LIANG Zi-sen, Mobile: +86-13602782072,Fax: +86-20-85280351, E-mail: liangzisen@scau.edu.cn
  • 作者简介:YU Zhan-mei, E-mail: jillsazhanmei@163.com;

Effect of Fuzheng Jiedu granule on immunological function and level of immune-related cytokines in immune-suppressed mice

 YU Zhan-mei, HUANG Xian-hui, YAN Chao-qun, GAO Jin, LIANG Zi-sen   

  1. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China
  • Received:2015-01-08 Online:2016-03-07 Published:2016-03-09
  • Contact: HUANG Xian-hui, Mobile: +86-13808842259,Fax: +86-20-87344801, E-mail: xhhuang@scau.edu.cn;LIANG Zi-sen, Mobile: +86-13602782072,Fax: +86-20-85280351, E-mail: liangzisen@scau.edu.cn
  • About author:YU Zhan-mei, E-mail: jillsazhanmei@163.com;

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摘要： Fuzheng Jiedu granule exhibits a number of health benefits and it is thought that the mechanisms involved in these effects are due to the modulation of immunity. In this article, we studied the effect of Fuzheng Jiedu granule on immunological function and the expression of immune-related cytokines in immune-suppressed mice. 72 mice were randomly divided into six groups, with 12 in each group. The control groups included an untreated group, a negative control group (Cyclophosphamide) and a positive control group (Astragalus polysaccharide). There were three treated groups, which were given different doses of Fuzheng Jiedu granule: a low dose (100 mg kg–1), a medium dose (400 mg kg–1) and a high dose (600 mg kg–1). With the exception of the untreated control animals, each group received an intraperitoneal injection of Cyclophosphamide (100 mg kg–1) for 3 days to establish the immune-suppressed model. Mice were then treated for 19 consecutive days and, 24 h after the last treatment, blood was taken for the eyeballs and serum separation was performed. Analysis was made of the levels of related cytokines (IgA, IgG, IgM, IL-6, IFN-γ, C3, C4 and TNF-α), the transformation of lymphocytes and the immune organ indexes. The results showed that Fuzheng Jiedu granule can improve the levels of cytokines, the rate of proliferation of lymphocytes and the immune organ indexes of immune-suppressed mice.

关键词: Fuzheng Jiedu granule , immune suppression , cytokines , lymphocytes , immune organ index

Abstract: Fuzheng Jiedu granule exhibits a number of health benefits and it is thought that the mechanisms involved in these effects are due to the modulation of immunity. In this article, we studied the effect of Fuzheng Jiedu granule on immunological function and the expression of immune-related cytokines in immune-suppressed mice. 72 mice were randomly divided into six groups, with 12 in each group. The control groups included an untreated group, a negative control group (Cyclophosphamide) and a positive control group (Astragalus polysaccharide). There were three treated groups, which were given different doses of Fuzheng Jiedu granule: a low dose (100 mg kg–1), a medium dose (400 mg kg–1) and a high dose (600 mg kg–1). With the exception of the untreated control animals, each group received an intraperitoneal injection of Cyclophosphamide (100 mg kg–1) for 3 days to establish the immune-suppressed model. Mice were then treated for 19 consecutive days and, 24 h after the last treatment, blood was taken for the eyeballs and serum separation was performed. Analysis was made of the levels of related cytokines (IgA, IgG, IgM, IL-6, IFN-γ, C3, C4 and TNF-α), the transformation of lymphocytes and the immune organ indexes. The results showed that Fuzheng Jiedu granule can improve the levels of cytokines, the rate of proliferation of lymphocytes and the immune organ indexes of immune-suppressed mice.

Key words: Fuzheng Jiedu granule , immune suppression , cytokines , lymphocytes , immune organ index