Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)

doi:10.1016/S2095-3119(14)60975-8

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1687-1696.DOI: 10.1016/S2095-3119(14)60975-8

Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)

XIAO Gui-qing, ZHANG Hai-wen, LU Xiang-yang, HUANG Rong-feng

1、College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R.China
2、Hunan Agricultural Bioengineering Research Institute, Changsha 410128, P.R.China
3、Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2014-11-05 出版日期:2015-09-03 发布日期:2015-09-05
通讯作者: ZHANG Hai-wen, Tel: +86-10-82106136,E-mail: zhanghaiwen@caas.cn; LU Xiang-yang,E-mail: xiangyangcn@163.com
作者简介:XIAO Gui-qing, E-mail: xhxgq22@163.com
基金资助:
This work was supported by the Major Special Foundation of Transgenic Plants in China (2013ZX001-003 and 2014ZX08009-15B).

Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)

XIAO Gui-qing, ZHANG Hai-wen, LU Xiang-yang, HUANG Rong-feng

1、College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R.China
2、Hunan Agricultural Bioengineering Research Institute, Changsha 410128, P.R.China
3、Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2014-11-05 Online:2015-09-03 Published:2015-09-05
Contact: ZHANG Hai-wen, Tel: +86-10-82106136,E-mail: zhanghaiwen@caas.cn; LU Xiang-yang,E-mail: xiangyangcn@163.com
About author:XIAO Gui-qing, E-mail: xhxgq22@163.com
Supported by:
This work was supported by the Major Special Foundation of Transgenic Plants in China (2013ZX001-003 and 2014ZX08009-15B).

摘要/Abstract

摘要： A novel rice lesion mimic mutant (LMM) was isolated from an ethane methyl sulfonate (EMS)-induced 02428 mutant bank. The mutant, tentatively designated as lmm6, develops necrotic lesions in the whole growth period along with changes in several important agronomic traits. We found that the initiation of the lesions was induced by light and cell death occurred in lmm6 accompanied with accumulation of reactive oxygen species (ROS). The lower chlorophyll content, soluble protein content and superoxide dismutase (SOD) activity, the higher malondialdehyde (MDA) content were detected in lmm6 than in the wild type (WT). Moreover, the observation by transmission electronic microscope (TEM) demonstrated that some organelles were damaged and the stroma lamella of chloroplast was irregular and loose in mesophyll cell of lmm6. In addition, lmm6 was more resistant than WT to rice blast fungus Magnaporthe grisea infection, which was consistent with increased expression of four genes involved in the defense-related reaction. Genetic analysis showed that mutant trait of lmm6 is inherited as a monogenic recessive nuclear gene located on the long arm of chromosome 6. Using simple sequence repeat (SSR) markers, the target gene was finally delimited to an interval of 80.8 kb between markers MM2359 and MM2370, containing 7 annotated genes. Taken together, our results provide the information to identify a new gene involved in rice lesion mimic, which will be helpful in clarifying the mechanism of cell death and disease resistance in rice.

关键词: rice , lesion mimic mutant , gene mapping , ROS , blast resistance

Abstract: A novel rice lesion mimic mutant (LMM) was isolated from an ethane methyl sulfonate (EMS)-induced 02428 mutant bank. The mutant, tentatively designated as lmm6, develops necrotic lesions in the whole growth period along with changes in several important agronomic traits. We found that the initiation of the lesions was induced by light and cell death occurred in lmm6 accompanied with accumulation of reactive oxygen species (ROS). The lower chlorophyll content, soluble protein content and superoxide dismutase (SOD) activity, the higher malondialdehyde (MDA) content were detected in lmm6 than in the wild type (WT). Moreover, the observation by transmission electronic microscope (TEM) demonstrated that some organelles were damaged and the stroma lamella of chloroplast was irregular and loose in mesophyll cell of lmm6. In addition, lmm6 was more resistant than WT to rice blast fungus Magnaporthe grisea infection, which was consistent with increased expression of four genes involved in the defense-related reaction. Genetic analysis showed that mutant trait of lmm6 is inherited as a monogenic recessive nuclear gene located on the long arm of chromosome 6. Using simple sequence repeat (SSR) markers, the target gene was finally delimited to an interval of 80.8 kb between markers MM2359 and MM2370, containing 7 annotated genes. Taken together, our results provide the information to identify a new gene involved in rice lesion mimic, which will be helpful in clarifying the mechanism of cell death and disease resistance in rice.

Key words: rice , lesion mimic mutant , gene mapping , ROS , blast resistance

XIAO Gui-qing, ZHANG Hai-wen, LU Xiang-yang, HUANG Rong-feng. Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2015, 14(9): 1687-1696.

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Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)

Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.)

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