落花生属植物遗传转化研究现状

doi:10.1016/j.jia.2024.06.010

摘要/Abstract

摘要：

花生是重要的植物油、蛋白质和饲用作物。花生隶属于落花生属植物，该属包括9个区组83种植物。多数落花生属植物为野生种，可以作为饲草和草坪草。另外，花生区组的野生种还能够为栽培花生提供基因资源用于提高其遗传多样性。至今，已鉴定到多个落花生属植物的重要基因资源。但是，却缺少基于分子水平的遗传改良，研究者归咎为落花生属植物遗传转化体系不成熟。然而，早在30年前就已有落花生属植物遗传转化的报道。因此，在落花生属植物遗传转化体系完备和候选基因未能验证之间存在强烈的矛盾。本综述对已有落花生属植物的体外再生体系和遗传转化体系进行了总结，期望为研究者提供参考。

Abstract:

Peanuts (Arachis hypogaea) are vegetable oil, protein and forage crops. The genus Arachis contains nine intrageneric taxonomic sections, including 83 species. Most Arachis species are wild plants widely serving as forage and turfgrass plants. Moreover, wild Arachis plants can provide gene resources for broadening genetic diversity in peanuts. To date, several key genes have been identified using recombinant inbred lines of Arachis species. The use of genetic engineering in improving peanut genetic diversity has not been popularized. This is because of the lack of a robust and reliable genetic transformation method for Arachis species. However, evidence indicates that the genetic transformation of Arachis plants had been reported 30 years ago. Therefore, there is a strong contradiction between the genetic transformation and the unsuccessful transfer of candidate genes into Arachis plants. This review summarized the Arachis regeneration and genetic transformation methods to deepen the understanding of transgenic technology in Arachis species.

Key words: Arachis , CRISPR/Cas9 , genetic transformation , in vitro regeneration

. 落花生属植物遗传转化研究现状[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.06.010.

Hui Song, Meiran Li, Zhenquan Duan. The current status of the genetic transformation of Arachis plants[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.06.010.

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