JIA-2019-11

2512 LI Li-shu et al. Journal of Integrative Agriculture 2019, 18(11): 2505–2513 is involved in abiotic stress. For example, in sugar beet, BveIF1A expression was not induced by NaCl treatment, but overexpression of BveIF1A increased the NaCl tolerance of transgenic A . thaliana lines (Rausell et al. 2003). Salt concentration affects eIF1 transcription levels in rice and F. rubra , and overexpressing OseIF1 enhanced salt tolerance (Diédhiou et al. 2008). LceIF1 was induced by pH and Na + concentration, and high LceIF1 expression in transgenic plants maintained high growth rates and allowed continued growth and development under salt stress conditions (Sun and Hong 2013). TheIF1A expression was significantly up-regulated by NaCl and PEG treatment, and overexpression of TheIF1A improved salt and osmotic stress tolerance in transgenic tobacco lines (Yang et al. 2017). In the present study, MieIF1A-b expression was regulated not only by salt treatment but also by cold, drought, H 2 O 2 , SA andABA treatment. These results indicated that MieIF1A-b may play an important role in responses to abiotic stress in mango. Compared to WT, overexpression of MieIF1A-b in transgenic A . thaliana plants enhanced vegetative growth under salt stress. Our findings demonstrated that MieIF1A-b may be correlated with the control of plant growth and salt adaptation, which is consistent with previous studies. 5. Conclusion The present study has shown that MieIF1A-b transcription was up-regulated with fruit development and induced by cold, salt, drought, H 2 O 2 , SA and ABA treatments. Transgenic plants that overexpressed MieIF1A-b showed enhanced vegetative growth under salt stress treatment. MieIF1A-b may be related to the fruit development and salt adaptation. But the stress mechanism and function of MieIF1A-b needs further study. Acknowledgements This research was supported by the National Natural Science Foundation of China (31660561), the Natural Science Foundation of Guangxi, China (2015GXNSFAA139052), the Key Research and Development Project of Guangxi, China (GXKJ-AB17292010), and theMajor Science andTechnology Projects of Guangxi, China (GXKJ-AA17204097-3 and GXKJ-AA172040 26-2), the Innovation Team of Guangxi Mango Industry Project, China (nycytxgxcxtd-06-02), and the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China (SKLCUSA-a201906). References Battiste J L, Pestova T V, Hellen C U T, Wagner G. 2000. The eIF1A solution structure reveals a large RNA-binding surface important for scanning function. Mollecular Cell , 5 , 109–119. Chaudhuri J, Si K, Maitra U. 1997. Function of eukaryotic translation initiation factor 1A ( eIF1A ) (formerly called eIF- 4C ) in initiation of protein synthesis. Journal of Biological Chemistry , 272 , 7883–7891. 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