JIA-2019-11

2511 LI Li-shu et al. Journal of Integrative Agriculture 2019, 18(11): 2505–2513 development and reached the highest transcript level in fruit 80 d after flowering. Our studies suggested that MieIF1A-b may regulate fruit development. Some studies have shown that the function of eIF1A A B 0 50 100 200 120 100 80 60 40 20 0 0 50 100 NaCl (mmol L –1 ) 200 Seedling livability (%) WT L10 L21 * * * * * L4 WT L10 L21 L4 WT L10 L21 L4 WT L10 L21 L4 WT L10 L21 L4 Fig. 4 Phenotypic (A–D) and statistical (E and F) analysis of plant height, root length, hypocotyl length and leaf area in transgenic Arabidopsis thaliana and wild-type plants. WT, wild-type A. thaliana . L4, L10, and L21 represent the three MieIF1A-b T 3 transgenic A. thaliana generations, respectively. Bar=1 mm approximately. Error bars indicate SE. * denotes significant differences ( P <0.05). A B D C E F MieIF1A-b WT Leaf area (mm 2 ) Length (mm) CK L4 L21 L10 CK L4 L21 L10 Plant Root Hypocotyl CK L4 L21 L10 4 * * * * * * * * * * * 2 1 0 3 5 8 4 2 0 6 1 mm 1 mm 1 mm WT L10 L21 L4 Fig. 5 Salt tolerance analysis in Arabidopsis thaliana . A, growth of A. thaliana under different salt concentrations in 1/2 medium: 0 , 50, 100 and 200 mmol L –1 NaCl, respectively. B, survival rate of A. thaliana and wild-type plants under different salt concentration treatments. WT, wild-type A. thaliana . L4, L10, and L21 represent the three MieIF1A-b T 3 transgenic A. thaliana generations, respectively. Error bars indicate SE. * denotes significant differences ( P <0.05).