JIA-2019-11

Journal of Integrative Agriculture 2019, 18(11): 2505–2513 RESEARCH ARTICLE Available online at www.sciencedirect.com ScienceDirect Molecular characterization, expression and function analysis of eukaryotic translation initiation factor ( eIF1A ) in Mangifera indica LI Li-shu 1, 2* , LUO Cong 1* , AN Zhen-yu 3 , LIU Zhao-liang 1 , DONG Long 3 , YU Hai-xia 1 , HE Xin-hua 1 1 The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Agriculture, Guangxi University, Nanning 530004, P.R.China 2 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China 3 Horticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China Abstract Eukaryotic translation initiation factor 1A ( eIF1A ) functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants. However, little is known about the eIF1A gene involved in fruit development and stress response of mango. In this study, the MieIF1A-b gene was isolated from Mangifera indica , and contains a 435-bp open reading frame, which encodes a putative protein of 144 amino acids (GenBank accession number: KP676599). The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6. Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis , Manihot esculenta , and Populus trichocarpa , with 96 and 95% identity, respectively. Quantitative reverse transcriptative PCR (qRT-PCR) analyses indicated that MieIF1A-b was expressed in all tested tissues, and had the highest expression level in fruit 80 d after flowering. The expression of MieIF1A-b was obviously regulated by NaCl and H 2 O 2 treatments in leaves. Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth, phenotype and salinity tolerance compared with wild-type (WT) plants. The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation, and it was a candidate gene for abiotic stress in mango. Keywords: Mangifera indica , MieIF1A-b gene, gene clone, expression, functional analysis 1. Introduction Protein synthesis is a dynamic, universal, essential process in all organisms, including plants (Sonenberg and Hinnebusch 2009). Eukaryotic translation initiation factors ( eIFs ), including six different families, eIF1 , eIF2 , eIF3 , eIF4 , eIF5 , and eIF6 , are involved in protein synthesis in eukaryotes (Grewal et al . 2012; Yang et al . 2017). Apart from protein synthesis, eIFs reportedly influence plant development and stress regulation. For example, eIF3 , eIF4A , eIF4E , eIF4G , and eIF5A are involved in vegetative and reproductive growth processes; eIF1A , e IF5A , and eIF4E Received 5 December, 2018 Accepted 7 July, 2019 LI Li-shu, E-mail: shukitty@126.com ; Correspondence HE Xin- hua, E-mail: honest66222@163.com * These authors contributed equally to this study. © 2019 CAAS. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) . doi: 10.1016/S2095-3119(19)62774-7