JIA-2019-11

2464 XU Bing-qin et al. Journal of Integrative Agriculture 2019, 18(11): 2457–2471 Fig. 4 Scatter plot of the top 20 Kyoto encyclopedia of genes and genomes (KEGG) enrichment results of differentially expressed genes (DEGs) in each pairwise comparison. RichFactor is the ratio of DEG numbers annoted in this pathway term to all gene numbers annotated in this pathway term. A large RichFactor represents greater intensiveness. The Q-value is the corrected P -value ranging from 0 to 1, and a lower Q-value represents greater intensity. Fig. 5 A Venn diagram showing number of differentially expressed genes (DGEs) co-modulated in DM1/DM2 and HN1/HN2 in leaves with the 24 h short-term drought stress treatment. B, number of Top 10 transcription factor (TF) family-encoding genes detected in DEGs of DM1/DM2 and HN1/HN2. ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● Ribosome Phenylpropanoid biosynthesis DNA replication Cutin, suberine and wax biosynthesis Insulin resistance Amino sugar and nucleotide sugar metabolism Glutathione metabolism Linoleic acid metabolism Ether lipid metabolism Plant hormone signal transduction Ribosome biogenesis in eukaryotes Glycerophospholipid metabolism Stilbenoid, diarylheptanoid and gingerol biosynthesis Fatty acid biosynthesis Thiamine metabolism Anthocyanin biosynthesis Diterpenoid biosynthesis Biotin metabolism Carotenoid biosynthesis Inositol phosphate metabolism 0.25 0.50 0.75 1.00 RichFactor Pathway Gene number ● ● 50 100 0.25 0.50 0.75 Q value Top 20 of pathway enrichment ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● Insulin resistance Galactose metabolism Fatty acid elongation Plant hormone signal transduction Cutin, suberine and wax biosynthesis Starch and sucrose metabolism Carotenoid biosynthesis Phenylpropanoid biosynthesis Glutathione metabolism Arginine and proline metabolism Ascorbate and aldarate metabolism alpha−Linolenic acid metabolism Regulation of autophagy Amino sugar and nucleotide sugar metabolism Glycolysis/Gluconeogenesis Valine, leucine and isoleucine degradation Linoleic acid metabolism Diterpenoid biosynthesis Stilbenoid, diarylheptanoid and gingerol biosynthesis Fatty acid degradation 0.10 0.15 0.20 0.25 RichFactor Pathway Gene number ● ● ● 10 20 30 0.1 0.2 Q value Top 20 of pathway enrichment DM1- vs .-DM2 HN1- vs .-HN2 2 576 938 1 102 39 30 24 23 20 19 18 13 12 10 0 10 20 30 40 bHLH WRKY NAC ERF MYB bZIP HD−ZIP C2H2 GRAS MYB-related TF family No. of gene Gene number of Top 10 TF family in two comparison groups B A