JIA-2019-11

2463 XU Bing-qin et al. Journal of Integrative Agriculture 2019, 18(11): 2457–2471 enriched in GO terms related to stress tolerance, responses to stimulus and responses to heat ( P <0.05) (Appendix E). Most of these genes were indicated to play crucial roles in protecting plant cells from the damage of water deficit, such as by maintaining correct protein folding and stabilization. According to the KEGG annotations, the co-modulated DEGs were categorized into 91 pathways (Appendix F) and the main pathways were plant hormone signal transduction (11.86%), phenylpropanoid biosynthesis (6.7%), protein processing in the endoplasmic reticulum (6.19%), starch and sucrose metabolism (6.19%) (Appendix G). 3.6. Drought stress responsive transcriptional factors (TFs) It is known that TF-encoding genes play key roles in the perception and transmission of external signals and also in plant defense responses to drought stress (Mickelbart et al . 2015). Overall, a total of 296 TF-encoding genes in 50 TF families were detected in DEGs of DM1/DM2 and Fig. 3 Gene Ontology (GO) based enrichment analysis ( P -value≤0.05) of differentially expressed genes (GEGS) in two comparison groups DM1<DM2(+) (A) and HN1<HN2(+) (B). X -axis represents GO terms; Y -axis indicates the number of DEGs. 0 226 452 678 904 1130 No. of genes Level2 GO terms of DM1<DM2(+) Up-regulated Down-regulated Biological process Cellular component Molecular function A 0 109 218 327 436 545 No. of genes Level2 GO terms of HN1<HN2(+) Up-regulated Down-regulated Biological process Cellular component Molecular function B Biological adhesion Biological regulation Cellular component organizationor biogenesis Cellular process Detoxification Developmental process Immune system process Growth Localization Locomotion Metabolic process Multi-organism process Multicellular organismal process Reproduction Reproductive process Response to stimulus Rhythmic process Signaling Single-organism process Cell Cell junction Cell part Extracellular region Extracellular region part Macromolecular complex Membrane Membrane part Membrane-enclosed lumen Nucleoid Organelle Organelle part Supramolecular fiber Virion Virion part Antioxidant activity Binding Catalytic activity Electron carrier activity Molecular function regulator Molecular transducer activity Nucleic acid binding transcription factor activity Signal transducer activity Structural molecule activity Transcription factor activity, protein binding Transporter activity Biological adhesion Biological regulation Cellular component organizationor biogenesis Cellular process Detoxification Developmental process Immune system process Growth Localization Locomotion Metabolic process Multi-organism process Multicellular organismal process Reproduction Reproductive process Response to stimulus Rhythmic process Signaling Single-organism process Cell junction Cell part Extracellular region Extracellular region part Macromolecular complex Membrane Membrane part Membrane-enclosed lumen Nucleoid Organelle Organelle part Supramolecular fiber Virion Virion part Antioxidant activity Binding Catalytic activity Electron carrier activity Molecular function regulator Molecular transducer activity Nucleic acid binding transcription factor activity Signal transducer activity Structural molecule activity Transcription factor activity, protein binding Transporter activity