JIA-2019-11

2670 MENG Di et al. Journal of Integrative Agriculture 2019, 18(11): 2668–2672 Table 1 Complete genome summary of Bacillus amyloliquefaciens groups Strain Genome size (bp) GC content (%) Protein-coding genes Isolate resource Y2 4238 624 45.9 4 038 Isolated from wheat rhizosphere SRCM101267 4 070 574 45.9 4 028 Isolated from food in South Korea ALB69 4 046 611 46.5 3 821 Isolated from almond drupe in Chico, California SH-B74 4 042 190 46.4 3 890 Isolated from marine sediment ALB65 4 041 665 46.4 3 823 Isolated from alfalfa silage in Davis, California UMAF6639 4 034 636 46.3 3 741 – YP6 4 009 619 45.9 4 210 Isolated from the rhizosphere of Lolium perenne L. on a rock phosphorus mine in Guizhou, China B15 4006 754 46.5 3 759 Isolated from grape in Xinjiang, China UMAF6614 4 005 145 46.5 3 754 – HK1 4 002 836 46.0 3 906 Isolated from corn stalk residue compost, China LL3 3995 227 45.7 3 941 Isolated from fermented food and presents the glutamic acid-independent production of poly-γ-glutamic acid LM2303 3 989 393 46.7 3 771 Isolated from wild yak dung in Qinghai-Tibetan Plateau, China ALB79 3 982 905 46.4 3 720 Isolated from grape in Berkeley, California DSM7 3 980 199 46.1 3 870 Representative strain MBE1283 3966 922 46.5 3 734 Isolated from Korean traditional alcoholic beverage in South Korea Y14 3957 164 46.4 3 741 Isolated from rhizosphere of peanut in Linyi, Shandong, China KHG19 3 953 361 46.6 3 698 – LFB112 3 942 754 46.7 3 684 – XH7 3 939 203 45.8 3 889 – TA208 3,937,511 45.8 3 891 – IT-45 3 928 857 46.6 3 735 – S499 3 927 922 46.6 3 729 Isolated from plant in Iturie Province, Democratic Republic of the Congo WS-8 3 929 787 46.5 3 670 Isolated from soil in Baoding, China B-4 3919 798 46.7 3 725 – CC178 3 916 828 46.5 3 702 – MT45 3 897 521 46.1 3 752 Isolated from Daqu in Maotai, Renhuai, China RD7-7 3 688 209 46.3 3 501 Isolated from fermented soybean paste in South Korea – indicates not found the isolate resource in NCBI datebase. Fig. 1 Abundance of clusters of orthologous groups (COG) categories. Black point indicates the abundance of YP6 for each category. A box and whisker plot indicates a statistical distribution of the COG categories of 8 B . amyloliquefacients genomes: B . amyloliquefaciens XH7 (CP002927.1), B . amyloliquefaciens LL3 (CP002634.1), B . amyloliquefaciens DSM7 (FN597644.1), B . amyloliquefaciens TA208 (CP002627.1), B . amyloliquefaciens strain SRCM101267 (CP021505.1), B . subtilis strain ATCC13952 (CP009748.1), B . amyloliquefaciens strain MT45 (CP011252.1) and B . amyloliquefaciens strain RD7-7 (CP016913.1). 0 50 100 150 200 250 300 L F M H J G Q P E K I D T O U V C N COG category Number of genes membrane to maintain cell osmotic balance. Meanwhile, the corresponding functions were also reduced as shown in the results above, resulting in energy saving and reproductive efficiency to adapt to the harsh living environment. In addition, B . amyloliquefaciens YP6 comprised a variety of promising genes (Appendix C), including solubilizing- phosphorus and OPs-degrading related genes ( pho D, pho A, phr C, pho E, ycs E, bcr C and yva K) (Sogorb and Vilanova 2002; Chu et al. 2018), IAA synthesis related genes ( amh X, cge E and eps M) (Idris et al. 2007), and siderophores synthesis related genes ( ent B, men F, ent C and ent A) (Sun et al. 2017), which made B . amyloliquefaciens YP6 to be more potential for agricultural production and environmental remediation.