JIA-2019-11

Journal of Integrative Agriculture 2019, 18(11): 2668–2672 SHORT COMMUNICATION Available online at www.sciencedirect.com ScienceDirect Complete genome sequence of Bacillus amyloliquefaciens YP6, a plant growth rhizobacterium efficiently degrading a wide range of organophosphorus pesticides MENG Di, ZHAI Li-xin, TIAN Qiao-peng, GUAN Zheng-bing, CAI Yu-jie, LIAO Xiang-ru Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P.R.China Abstract Bacillus amyloliquefaciens YP6, a plant growth promoting rhizobacteria, is capable of efficiently degrading a wide range of organophosphorus pesticides (OPs). Here, we report the complete genome sequence of this bacterium with a genome size of 4009 619 bp, 4 210 protein-coding genes and an average GC content of 45.9%. Based on the genome sequence, several genes previously described as being involved in solubilizing-phosphorus, OPs-degradation, indole-3-acetic acid (IAA) and siderophores synthesis. Interestingly, compared with the genomes of B . amyloliquefaciens species, strain YP6 had larger genome size and the most protein-coding genes. Moreover, the four categories of “cell envelope biogenesis, outer membrane (M),” “translation, ribosomal structure and biogenesis (J),” “transcription (K),” and “signal transduction mechanisms (T)” were fewer. These differences may be related to extensive environmental adaptability of the genus B . amyloliquefaciens . These results expand the application potential of strain YP6 for environmental bioremediation, provide gene resources involved in OPs degradation for biotechnology and gene engineering, and contribute to provide insights into the relationship between microorganism and living environment. Keywords: organophosphorus pesticides, genome sequence, plant growth promoting rhizobacteria, Bacillus amyloliquefaciens increase food production and prevent disease transmission (Zhang et al. 2016). While, the indiscriminate and irresponsible usage of OPs in agriculture results in approximately 3 million poisonings and 200 000 deaths of OPs annually (Karalliedde and Senanayake 1989; Sogorb et al. 2004; Singh and Walker 2006; Yadav et al. 2015). Hence, degrading or eliminating OPs pollution from environments is imperative. As the result of biotechnological development, new efforts have been undertaken to emphasize the necessity of using microorganisms for degrading pollutants rather than their disposal (Singh and Walker 2006; Yang et al. 2011; Duraisamy et al. 2018). Recently, various microorganisms capable of degrading OPs have been isolated successfully, including Penicillium decaturense (Alvarenga et al. 2014), Received 26 September, 2018 Accepted 27 February, 2019 MENG Di, Mobile: +86-15852833873, E-mail: mengdi030321 @126.com; Correspondence LIAO Xiang-ru, Mobile: +86- 13771104596, E-mail: liaoxiangru@163.com © 2019 CAAS. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) . doi: 10.1016/S2095-3119(19)62658-4 1. Introduction Organophosphorus pesticides (OPs) are a group of toxic chemicals widely used in agriculture as insecticides to