JIA-2019-11

2585 CUI Dong-nan et al. Journal of Integrative Agriculture 2019, 18(11): 2579–2588 Drosophila melanogaster (Scott et al. 1998; Gilbert and Warren 2005) and in the fall webworm, Hyphantria cunea Drury, cytochrome P450 is involved in detoxification and metabolism as indicated by down-regulated mRNA levels of cytochrome P450 in diapause pupae (Tijet et al. 2001; Deng et al. 2018). In this research, cytochrome P450 was found in both non-diapause and diapause eggs, with expression in diapause eggs significantly down-regulated. Moreover, at the transcriptional level, cytochrome P450 in diapause oocytes was significantly lower than the non- diapause cohort, which was consistent with the data at the protein level. KEGG pathway analysis showed that peroxisome was a highly and significantly enriched pathway. Peroxisome, a subcellular organelle, can catalyze various metabolic reactions, such as fatty-acid β-oxidation, glyoxylate detoxification and plasmalogen biosynthesis (Wanders 2014). Our studies showed that the expression of proteins related to peroxisome differed between the diapause and non-diapause eggs in migratory locust. It is evident that genes involved in β-oxidation were strongly suppressed in early diapause (Poelchau et al. 2013; Hao et al. 2016). Multifunctional enzymes are critical in the second and third reactions of the peroxisomal β-oxidation cycle. In D . melanogaster , multifunctional enzyme type 2, a peroxisomal protein, is active in the β-oxidation of fatty Table 4 Substantially enriched pathways identified by KEGG pathway analysis No. Pathway ID DEPs number Percentage (%) 1) P -value 1 Metabolic pathways dme01100 44 25.14 3.31E-18 2 Carbon metabolism dme01200 11 6.29 2.84E-08 3 Protein processing in endoplasmic reticulum dme04141 11 6.29 7.60E-08 4 Beta-Alanine metabolism dme00410 5 2.86 2.46E-06 5 Glutathione metabolism dme00480 7 4.00 4.89E-06 6 Fatty acid degradation dme00071 5 2.86 2.16E-05 7 Valine, leucine and isoleucine degradation dme00280 5 2.86 2.85E-05 8 Drug metabolism-cytochrome P450 dme00982 6 3.43 5.30E-05 9 Metabolism of xenobiotics by cytochrome P450 dme00980 6 3.43 5.30E-05 10 Biosynthesis of amino acids dme01230 6 3.43 6.25E-05 11 Fatty acid metabolism dme01212 5 2.86 9.27E-05 12 Histidine metabolism dme00340 3 1.71 1.43E-04 13 Phagosome dme04145 6 3.43 1.62E-04 14 Cysteine and methionine metabolism dme00270 4 2.29 4.07E-04 15 Arachidonic acid metabolism dme00590 3 1.71 5.65E-04 16 Oxidative phosphorylation dme00190 7 4.00 5.86E-04 17 Proteasome dme03050 4 2.29 1.91E-03 18 Glycolysis/Gluconeogenesis dme00010 4 2.29 2.33E-03 19 RNA transport dme03013 6 3.43 3.04E-03 20 Fructose and mannose metabolism dme00051 3 1.71 3.07E-03 21 Phenylalanine metabolism dme00360 2 1.14 3.27E-03 22 mRNA surveillance pathway dme03015 4 2.29 5.97E-03 23 Peroxisome dme04146 4 2.29 5.97E-03 24 Ribosome dme03010 7 4.00 8.64E-03 25 Spliceosome dme03040 5 2.86 9.05E-03 26 Fatty acid elongation dme00062 2 1.14 9.18E-03 27 Citrate cycle (TCA cycle) dme00020 3 1.71 1.02E-02 28 Pyruvate metabolism dme00620 3 1.71 1.15E-02 29 Insect hormone biosynthesis dme00981 2 1.14 1.17E-02 30 Pentose and glucuronate interconversions dme00040 3 1.71 1.22E-02 31 Glycerolipid metabolism dme00561 3 1.71 1.29E-02 32 Butanoate metabolism dme00650 2 1.14 1.31E-02 33 Tyrosine metabolism dme00350 2 1.14 1.31E-02 34 Lysosome dme04142 4 2.29 1.51E-02 35 2-Oxocarboxylic acid metabolism dme01210 2 1.14 1.61E-02 36 Tryptophan metabolism dme00380 2 1.14 1.77E-02 37 Propanoate metabolism dme00640 2 1.14 2.10E-02 38 Pentose phosphate pathway dme00030 2 1.14 2.28E-02 39 Glycine, serine and threonine metabolism dme00260 2 1.14 3.46E-02 40 Alanine, aspartate and glutamate metabolism dme00250 2 1.14 3.67E-02 1) Percentage (%)=The number of differentially enriched proteins in the pathway/The total number of differentially expressed proteins