JIA-2019-11

Journal of Integrative Agriculture 2019, 18(11): 2579–2588 RESEARCH ARTICLE Available online at www.sciencedirect.com ScienceDirect Identification of diapause-associated proteins in migratory locust, Locusta migratoria L. (Orthoptera: Acridoidea) by label-free quantification analysis CUI Dong-nan 1* , TU Xiong-bing 1* , HAO Kun 1 , Aftab Raza 1 , CHEN Jun 1 , Mark McNeill 2 , ZHANG Ze-hua 1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China 2 AgResearch Ltd., Christchurch 8140, New Zealand Abstract Maternal photoperiodic response is a key factor that affects offspring diapause in migratory locust, Locusta migratoria L. (Orthoptera: Acridoidea). Although many aspects of insect diapause have been studied, little is known about the molecular mechanisms of maternal photoperiodic response that influence diapause regulation. To gain insight into the possible mechanisms of maternal photoperiod influence on diapause regulation, proteomics data by label-free quantification analysis were generated from non-diapause and diapause eggs. A total of 175 proteins were differentially expressed between diapause and non-diapause eggs. Among them, 24 proteins were upregulated, and 151 proteins were downregulated. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichments were performed on all differentially expressed proteins (DEPs) and showed that peroxisome, insect hormone biosynthesis, and longevity regulating pathway may be related to diapause of migratory locust. Furthermore, we used qRT-PCR to verify some results of the proteomic analysis. Proteins such as hexamerin-like protein 4, juvenile hormone epoxide hydrolase 1 (JHEH1), cytochrome P450 and heat shock protein (HSP) 20.7 were predicted to be involved in diapause. This study provides an important reference for future research that will explore the mechanisms of diapause induced by maternal effects in migratory locust. Keywords: diapause, proteome, maternal effect, molecular mechanism 1. Introduction Diapause is a specific strategy of insect survival under unfavorable conditions including short photoperiods, cool temperature and drought (Denlinger andArmbruster 2014). There are two forms of insect diapause: facultative and obligatory diapause. Facultative diapause is programmed by environmental tokens such as daylength, while obligatory diapause, a mandatory, fixed component of ontogeny, is independent of environmental condition (Denlinger 2002). Received 25 September, 2018 Accepted 10 December, 2018 CUI Dong-nan, E-mail: cuidongnan88@163.com ; TU Xiong- bing, E-mail: xbtu@ippcaas.cn ; Correspondence Mark McNeill, mark.mcneill@agresearch.co.nz; ZHANG Ze-hua, E-mail: zhangzehua@caas.cn * These authors contributed equally to this study. © 2019 CAAS. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) . doi: 10.1016/S2095-3119(19)62607-9