JIA-2019-11

Journal of Integrative Agriculture 2019, 18(11): 2571–2578 RESEARCH ARTICLE Available online at www.sciencedirect.com ScienceDirect Characterization of field-evolved resistance to cyantraniliprole in Bemisia tabaci MED from China WANG Ran 1* , WANG Jin-da 2* , CHE Wu-nan 3 , SUN Yan 4 , LI Wen-xiang 5 , LUO Chen 1 1 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China 2 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agricultural and Forestry University, Fuzhou 350002, P.R.China 3 Department of Pesticide Sciences, Shenyang Agricultural University, Shenyang 110866, P.R.China 4 Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, P.R.China 5 College of Agriculture and Forestry Technology, Hebei North University, Zhangjiakou 075000, P.R.China Abstract Cyantraniliprole is a novel anthranilic diamide insecticide with significant efficacy against Bemisia tabaci , an important pest insect worldwide. In this study, we conducted reversion and selection work and genetic analysis, and determined cross-resistance spectrum and synergism of cyantraniliprole resistance based on the reported population, SX population, of B. tabaci collected from Shanxi Province, China. Compared with a susceptible strain (MED-S), SX population, the field-evolved cyantraniliprole-resistant population exhibited 26.4-fold higher resistance to cyantraniliprole. In SX, a sharp decline of cyantraniliprole resistance was shown in the absence of selection. Another tested strain, SX-R, was established from SX population after successive selection with cyantraniliprole and recently developed 138.4-fold high resistance to cyantraniliprole. SX-R had no cross-resistance to abamectin, imidacloprid, thiamethoxam, sulfoxaflor, or bifenthrin. Genetic analysis illustrated that cyantraniliprole resistance in SX-R was autosomally inherited and incompletely dominant. Additionally, piperonyl butoxide (PBO) significantly inhibited cyantraniliprole resistance in the SX-R strain. In conclusion, the selection of SX with cyantraniliprole led to high resistance to cyantraniliprole which is incompletely dominant and no cross-resistance to several common types of insecticides. Enhanced oxidative metabolism is possibly involved in the resistance of SX-R, yet target-site resistance could not be excluded. Keywords: Bemisia tabaci , cyantraniliprole, cross-resistance, inheritance, synergism 1. Introduction The tobacco whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is an important pest with a global distribution that has been reported to damage over 600 plant species, mainly by phloem-feeding (Liu et al. 2007; De Barro et al. 2011). B. tabaci not only damages plants directly but also transmits around 100 plant viruses (Hogenhout et al. 2008). Development of resistance to conventional insecticides has Received 19 October, 2018 Accepted 21 November, 2018 WANG Ran, Tel: +86-10-51503338, E-mail: rwang1105@126. com; Correspondence LUO Chen, Tel: +86-10-51503338, E-mail: luochen1010@126.com * These authors contributed equally to this study. © 2019 CAAS. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) . doi: 10.1016/S2095-3119(19)62557-8