JIA-2019-11

2554 LI Liu et al. Journal of Integrative Agriculture 2019, 18(11): 2549–2560 ASPV-LYC tightly clustered together with the isolate FJ-12, and the two isolates together with isolates Aurora-1 and CYD formed a clade, which had relatively close phylogenetic distance with the clade represented by isolates KL1 and KL9, but was distal to HB-HN1. Multiple alignment for CP sequences of ASPV-LYC, FJ-12, other ASPV isolates and Fig. 1 Phylogenetic analysis of Apple stem pitting virus (ASPV) isolates based on the nucleotide sequences of their genome (A) and coat protein gene (B). All available genome sequences of ASPV isolates and two apple green crinkle associated virus (AGCaV) isolates (Aurora-1 and CYD) are included in the genome-based analysis, and CP sequences of selected ASPV isolates are included in the CP analysis. Sequences previously reported from our group and referred from GenBank are identified by their GenBank accession numbers and hosts following isolate names. Isolates with available full genome sequences are in bold. The isolate LYC sequenced in the present study is indicated by “ ◆ ”. Cherry green ring mottle virus (CGRMV) in the genus Foveavirus was as an out group in each tree. The tree was constructed with MEGA 7.0.14 Program using the Maximum Likelihood method (Kumar et al. 2016). The numbers at the nodes indicate the percentage of 1000 bootstraps occurred in this clade. Values below 60% were suppressed. The bar represents 0.1 substitution per site.