JIA-2018-09

1940 HAN Jiao et al. Journal of Integrative Agriculture 2018, 17(9): 1932–1945 198 genes were differentially expressed in the transgenic lines, and 154 genes of these differentially expressed genes were up-regulated and 44 genes were down- regulated comparing to the wild type (Fig. 7-B). To profile the expressions of the McPht gene’s homologous gene in the transgenic rice line under phosphorus deficiency, we performed qRT-PCR to determine the mRNA accumulation of the Os04g0448800 gene, a mitochondrial carrier protein gene (accession no. AP014960.1). Data showed that both the transgenic line and the wild type have no obvious differences in mRNA accumulations of the Os04g0448800 gene in the shoots (Fig. 7-C). Additionally, we selected six genes of the differentially expressed genes, which were up-regulated in the transgenic rice (Table 2), to determine the expression profiles of these genes in response to phosphorus deficiency. qRT-PCR showed that the transgenic rice exhibits an enhanced increase in mRNA accumulations of these differentially expressed genes comparing to the wild type, and the relative expression levels of Os02g0602300, Os07g0650600, Os02g0106100, Os03g0348200, Os10g0190800, and Os08g0157500 genes increased by 3.38, 1.52, 0.55, 1.37, 1.09, and 0.23 folds in response to phosphorus deficiency, respectively (Fig. 7-C). This result seems to be identical with the RNAseq analyses of transcriptome (Table 2). As shown in Table 2, these differentially expressed genes are mainly involved in carbohydrate metabolic process, oxidoreductase activity, flavone 3’-O-methyltransferase 1, magnesium ion binding, isoaspartyl peptidase/L-asparaginase 2, alpha-humulene synthase, threonine-protein kinase, transmembrane 0 1.0 2.0 3.0 4.0 5.0 Os04g0448800 Os07g0650600 Os03g0348200 Gene Os08g0157500 Relative expression WT A C Line1 B 816 21 119 459 50 40 30 20 10 5 0 log 2 Fold (OE/WT) Diff Down noDEG Up 5 0 –log 10 FDR OE WT Fig. 7 Expression profiles and distributions of endogenous genes in the transgenic rice. A, gene expression of Venn diagrams. Each circle represents the total number of detected genes, and the overlapped portions of the circles indicate the detected genes in both samples. OE, line 1 (OE-1); WT, wild type. B, the distribution of differentially expressed genes (DEGs). Each point represents a gene, and red points represent the genes of up-regulation, blue points represent the genes of down-regulation, and green points represent the co-expressed genes. FDR, false discovery rate. Diff, differential expression. C, relative expression levels of endogenous genes in rice under phosphate stress. The WT was used as the control. All data represent mean±SD of three replicates.