JIA-2018-09

1939 HAN Jiao et al. Journal of Integrative Agriculture 2018, 17(9): 1932–1945 rice significantly higher than those of the wild type under phosphorus deficiency (Fig. 6-C). While the roots biomass of the transgenic rice seems to be corresponding to the changes of roots architecture (Fig. 6-D). These data indicated that the transgenic lines carrying the McPht gene might induce the changes of roots morphology in response to phosphorus deficiency. Physiological measurements showed that the roots activities of the transgenic rice were higher than those of the wild type under phosphorus deficiency. Comparing to wild- type, the root activities of the transgenic lines OE-1, OE-5, and OE-7 were increased by 0.53, 0.47, and 1.27 folds under phosphorus deficiency, respectively (Fig. 6-E). However, the wild type demonstrated higher roots activities than the transgenic rice under sufficient phosphorus supply. Both sufficient phosphorus supply and phosphorus deficiency led to an enhanced increase in the contents of total phosphate in the transgenic lines compared with the wild type (Fig. 6-F). Under phosphorus deficiency, the transgenic rice lines, OE-1, OE-5, and OE-7, separately showed an increase of 1.24, 1.19, and 1.15 mg g –1 in phosphate uptake per plant (Fig. 6-F). 3.4. Relative expressions of differentially expressed gene Transcriptome sequencing showed that total 21578 genes in the wild type and 21 935 genes in the transgenic lines were detected, total 21 119 genes were co-expressed, total 459 genes were differentially expressed in the wild type and total 816 genes were differentially expressed in the transgenic lines in response to phosphorus deficiency, respectively (Fig. 7-A). Statistical analyses showed that total A WT 1 WT 1 B 5 7 5 7 WT 1 5 7 WT 1 5 7 D C Fig. 5 Phenotypic images of the transgenic lines and the wild type. A and C, phenotypes of the wild type and the transgenic rice OE-1, OE-5, and OE-7 under supply of sufficient phosphorus. B and D, phenotypic images of the wild type and the transgenic rice OE-1, OE-5, and OE-7 under phosphorus deficiency. 0.0 10.0 20.0 30.0 40.0 50.0 60.0 A B C D E F Sufficient phosphorus Phosphorus deficiency Total root length (cm) WT OE-1 OE-5 OE-7 0.0 20.0 40.0 60.0 80.0 100.0 120.0 140.0 Sufficient phosphorus Phosphorus deficiency Number of lateral roots 0.0 1.6 3.2 4.8 6.4 8.0 Sufficient phosphorus Phosphorus deficiency Total projected area (cm 2 ) 0.0 0.3 0.6 0.9 1.2 Sufficient phosphorus Phosphorus deficiency Root weight (g) 0.0 0.1 0.2 0.3 0.4 0.5 Sufficient phosphorus Phosphorus deficiency Sufficient phosphorus Phosphorus deficiency Root activity (mg g –1 h –1 ) 0.0 6.0 12.0 18.0 24.0 30.0 Phosphorus content per plant (mg g –1 ) Fig. 6 Morphologic parameters and activities of the roots and total phosphate contents. A, the total roots length. B, the number of lateral roots. C, total roots projected area. D, roots weights. E, the roots activities. F, total phosphate contents per plant. All data represent mean±SD of three replicates.