JIA-2018-09

1937 HAN Jiao et al. Journal of Integrative Agriculture 2018, 17(9): 1932–1945 to exhibit strong fluorescence signals on the mitochondria, but the transformant carrying the 16318hGFP vector revealed entire green fluorescence signals throughout Arabidopsis protoplasts, suggesting that the McPht protein was very likely localized to the mitochondria. 3.2. Relative expression profiles of the McPht gene under phosphorus deficiency To profile the relative expressions of the McPht gene in both M. crystallinum and the transgenic rice under phosphorus deficiency, the quantitative PCR procedures were performed. The data showed that phosphorus deficiency affected the expression profiles of the McPht gene in roots and shoots of plants. As shown in Fig. 4-A, phosphorus deficiency induced the expression of the McPht gene in the roots of the transgenic lines OE-1, OE-5, and OE-7, and the transcripts of the McPht gene increased by 8.96, 17.21, and 1.32 folds comparing to sufficient phosphate supply, respectively. However, the relative expression levels of the McPht gene in the shoots of the transgenic lines seemed to be remarkably lower than those in the roots under phosphorus deficiency. Especially, the OE-1 line showed an enhanced decrease of 0.79 folds in the mRNA accumulations of the McPht gene in the shoots, and both the OE-5 and OE-7 lines slightly increased the mRNA accumulations of the McPht gene by 1.02 and 0.97 folds relative to those under sufficient phosphorus supply, respectively (Fig. 4-B). Similarly, the relative expression of the McPht gene in the shoots of M. crystallinum were significantly higher than those in the roots under phosphorus deficiency (Fig. 4-C). These data suggested that mRNA transcripts of the McPht gene in the 27 McPht 65 NtPht 88 GhPht 26 GrPht 25 StPht 25 SlPht 25 SPPht 29 GmPht 24 BnPht 24 AtPht 24 ThPht Consensus McPht NtPht GhPht GrPht StPht SlPht SPPht GmPht BnPht AtPht ThPht Consensus McPht NtPht GhPht GrPht StPht SlPht SPPht GmPht BnPht AtPht ThPht Consensus McPht NtPht GhPht GrPht StPht SlPht SPPht GmPht BnPht AtPht ThPht Consensus McPht NtPht GhPht GrPht StPht SlPht SPPht GmPht BnPht AtPht ThPht Consensus MVRI NHARVFFPI SFVVPNNKAGN M V C C F F R N S T K E E F L G L K G R E E R K E Q E N K P V K F G S I P P L I Q S P S S L P F I I S F P S R R F F R H S F L S S V A F P P S P Q P T A M M H M M A A F A A L V A I I P F M D D M M M D E E S S E E E S M M M N N D D N N N S E E E P S S S S S S S S S S R R F F R R R R S P P K R K K R R R R K K R S Q Q Q Q Q Q N N N N P S S S S S S S S S S L L L L L L L L L L F I L L L L L L I I I I p P P P P P P P P P P P s S S S S S S S S S S S f F F F F F F F F F F F L L L L L L L I L L L y Y Y Y Y Y Y Y Y Y Y Y S T S S S S S S S S S S P . . P P P S S S S . . . . T T T S S S S R S S S S S S S S S S S S A A S S S K S S S F F P P F F F T P P P G S N N S S S L R R R I S S S S S S P S S A D F F F L L L L V F F Q T S S T T T H L L L 108 144 178 116 98 95 95 110 98 112 108 Q P L L P P P H L L L M K D D K K K M D D D A T R R T T T L Q Q Q V F L L L L L N . V V S A L I A A A T . L L S V N N V V V A . N N P T A A P P P S . S S T E N N E E E A . N N L N S S . . . A . S P . . P P . . . . . N . . . A A . . . . . A . . . F F . . . . . A . . . S S . . . . . . . . . A A . . . . . . . . . S S . . . . . F . . . R R . . . . K E N . . S S . . . . N K T L T T T T T T A S S T S T I I T T T A P P F S S P P N S N A S S S A V G G V V V A P P N R L A A L P P P S A V S P P P P . . S P P E G A A A P . . P K R K G A A A A . . P . S S G Q S S . . . P . S P G P S S . . . A . P S G S S S . . . N . T P G S P P . . . L T S T E T S S . . . D M M V G . I I . . . A A I L G . K K . . . G S S S A N A A N N N S R R N K K R R N N N R K K K S S T T N N N G N N S F F F F L L L L F F F L V M M L I I M L L L i I I I I I I I I I I I P P P P P P P P A A A S A S S A A A S S S S p P P P P P P P P P P P A S N N T N N S T T S e E E E E E E E E E E E S P P P P P P S P P P S G G G G G G R G G G k K K K K K K K K K K K . . K K . . . . G G K i I I I I I I I I I I I e E E E E E E E E E E E M M M M M M M L M M M y Y Y Y Y Y Y Y Y Y Y Y s S S S S S S S S S S S P P P P P P P P R P P Q Q Q Q Q Q Q A D A A f F F F F F F F F F F F y Y Y Y Y Y Y Y Y Y Y Y a A A A A A A A A A A A a A A A A A A A A A A A c C C C C C C C C C C C t T T T T T T T T T T T V F F F F F F A V F F g G G G G G G G G G G G g G G G G G G G G G G G i I I I I I I I I I I I l L L L L L L L L L L L s S S S S S S S S S S S c C C C C C C C C C C C g G G G G G G G G G G G l L L L L L L L L L L L t T T T T T T T T T T T h H H H H H H H H H H H m M M M M M M M M M M M A A A A A A A T A T A v V V V V V V V V V V V t T T T T T T T T T T T p P P P P P P P P P P P l L L L L L L L L L L L d D D D D D D D D D D D l L L L L L L L L L L L v V V V V V V V V V V V k K K K K K K K K K K K c C C C C C C C C C C C n N N N N N N N N N N N m M M M M M M M M M M M q Q Q Q Q Q Q Q Q Q Q Q i I I I I I I I I I I I d D D D D D D D D D D D p P P P P P P P P P P P a A A A A A A A A A A A k K K K K K K K K K K K 198 234 268 206 188 185 185 200 188 202 198 y Y Y Y Y Y Y Y Y Y Y Y k K K K K K K K K K K K s S S S S S S S S S S S i I I I I I I I I I I I s S S S S S S S S S S S s S S S S S S S S S S S g G G G G G G G G G G G f F F F F F F F F F F F g G G G G G G G G G G G V V V V I V V V I I V l L L L L L L L L L L L l L L L L L L L L L L L k K K K K K K K K K K K e E E E E E E E E E E E q Q Q Q Q Q Q Q Q Q Q Q g G G G G G G G G G G G A A V V P P P F V V V R K R R R R R R R K R g G G G G G G G G G G G f F F F F F F F F F F F f F F F F F F F F F F F r R R R R R R R R R R R g G G G G G G G G G G G w W W W W W W W W W W W v V V V V V V V V V V V p P P P P P P P P P P P t T T T T T T T T T T T l L L L L L L L L L L L l L L L L L L L L L L L g G G G G G G G G G G G y Y Y Y Y Y Y Y Y Y Y Y s S S S S S S S S S S S a A A A A A A A A A A A q Q Q Q Q Q Q Q Q Q Q Q g G G G G G G G G G G G a A A A A A A A A A A A c C C C C C C C C C C C k K K K K K K K K K K K f F F F F F F F F F F F g G G G G G G G G G G G f F F F F F F F F F F F y Y Y Y Y Y Y Y Y Y Y Y e E E E E E E E E E E E F F F F F F F F F Y F f F F F F F F F F F F F k K K K K K K K K K K K k K K K K K K K K K K K T Y Y Y Y Y Y Y Y T Y y Y Y Y Y Y Y Y Y Y Y Y s S S S S S S S S S S S d D D D D D D D D D D D M L L L L L L I L L L a A A A A A A A A A A A g G G G G G G G G G G G P A P P A A A P P P P e E E E E E E E E E E E N N Y Y N N N Y F Y Y A A F F A A A A A T F A A A A A A A S A A S k K K K K K K K K K K K y Y Y Y Y Y Y Y Y Y Y Y k K K K K K K K K K K K t T T T T T T T T T T T l L L L L L L L L L L L i I I I I I I I I I I I y Y Y Y Y Y Y Y Y Y Y Y l L L L L L L L L L L L a A A A A A A A A A A A g G G G G G G G G G G G s S S S S S S S S S S S a A A A A A A A A A A A s S S S S S S S S S S S a A A A A A A A A A A A e E E E E E E E E E E E V V V V V V V V V I V i I I I I I I I I I I I a A A A A A A A A A A A d D D D D D D D D D D D V I V V I I I I V I V a A A A A A A A A A A A l L L L L L L L L L L L c C C C C C C C C C C C p P P P P P P P P P P P L F F F F F F F F F F e E E E E E E E E E E E a A A A A A A A A A A A v V V V V V V V V V V V k K K K K K K K K K K K v V V V V V V V V V V V r R R R R R R R R R R R 288 324 358 296 278 275 275 290 278 292 288 v V V V V V V V V V V V q Q Q Q Q Q Q Q Q Q Q Q t T T T T T T T T T T T q Q Q Q Q Q Q Q Q Q Q Q p P P P P P P P P P P P g G G G G G G G G G G G f F F F F F F F F F F F a A A A A A A A A A A A r R R R R R R R R R R R g G G G G G G G G G G G L L L L L L L L M M M s S S S S S S S S S S S d D D D D D D D D D D D g G G G G G G G G G G G L L L L L L L L F F F p P P P P P P P P P P P k K K K K K K K K K K K f F F F F F F F F F F F I V V V V V V V V I V K R R R R R R R K K K s S S S S S S S S S S S e E E E E E E E E E E E g G G G G G G G G G G G A T A A P P P T Y Y V G M L L M M M L G G G g G G G G G G G G G G G l L L L L L L L L L L L y Y Y Y Y Y Y Y Y Y Y Y k K K K K K K K K K K K g G G G G G G G G G G G L L I I L L L L L L L V V V V V V V V A A G p P P P P P P P P P P P l L L L L L L L L L L L w W W W W W W W W W W W g G G G G G G G G G G G r R R R R R R R R R R R q Q Q Q Q Q Q Q Q Q Q Q i I I I I I I I I I I I p P P P P P P P P P P P y Y Y Y Y Y Y Y Y Y Y Y t T T T T T T T T T T T m M M M M M M M M M M M m M M M M M M M M M M M k K K K K K K K K K K K f F F F F F F F F F F F a A A A A A A A A A A A s S S S S S S S S S S S f F F F F F F F F F F F e E E E E E E E E E E E t T T T T T T T T T T T i I I I I I I I I I I I v V V V V V V V V V V V e E E E E E E E E E E E N M M M M M M L M M M i I I I I I I I I I I I y Y Y Y Y Y Y Y Y Y Y Y k K K K K K K K K K K K H H Y Y H H H H Y Y Y A A G G A A A A A A A I V I I V V V I I I I p P P P P P P P P P P P T K T T K K K T N N T p P P P P P P P P P P P k K K K K K K K K K K K N N E E N N N N H S E E E Q Q E E E E E E Q c C C C C C C C C C C C S S S S S S S T S S S k K K K K K K K K K K K G S N N S S S S G G N L M L L M M M L L L L q Q Q Q Q Q Q Q Q Q Q Q l L L L L L L L L L L L g G G G G G G G G G G G V I V V I I I V V V V s S S S S S S S S S S S f F F F F F F F F F F F a A A A A A A A A A A A g G G G G G G G G G G G g G G G G G G G G G G G y Y Y Y Y Y Y Y Y Y Y Y V I V V I I I V V V V a A A A A A A A A A A A g G G G G G G G G G G G v V V V V V V V V V V V F F F F F F F L F F F c C C C C C C C C C C C a A A A A A A A A A A A i I I I I I I I I I I I 357 406 440 378 360 357 357 374 356 375 369 v V V V V V V V V V V V s S S S S S S S S S S S h H H H H H H H H H H H p P P P P P P P P P P P a A A A A A A A A A A A d D D D D D D D D D D D n N N N N N N N N N N N l L L L L L L L L L L L v V V V V V V V V V V V s S S S S S S S S S S S f F F F F F F F F F F F l L L L L L L L L L L L n N N N N N N N N N N N n N N N N N N N N N N N a A A A A A A A A A A A k K K K K K K K K K K K g G G G G G G G G G G G a A A A A A A A A A A A t T T T T T T T T T T T v V V V V V V V V V V V g G G G G G G G G G G G d D D D D D D D D D D D a A A A A A A A A A A A v V V V V V V V V V V V k K K K K K K K K K K K k K K K K K K K K K K K I I L L I I I L I I L g G G G G G G G G G G G V V L L V V V L M M L V V W W L L L W V V W g G G G G G G G G G G G l L L L L L L L L L L L f F F F F F F F F F F F t T T T T T T T T T T T r R R R R R R R R R R R g G G G G G G G G G G G l L L L L L L L L L L L p P P P P P P P P P P P l L L L L L L L L L L L r R R R R R R R R R R R i I I I I I I I I I I I v V V V V V V V V V V V m M M M M M M M M M M M i I I I I I I I I I I I g G G G G G G G G G G G t T T T T T T T T T T T l L L L L L L L L L L L t T T T T T T T T T T T g G G G G G G G G G G G a A A A A A A A A A A A q Q Q Q Q Q Q Q Q Q Q Q w W W W W W W W W W W W g G G G G G G G G G G G I I I I I I I I L L L y Y Y Y Y Y Y Y Y Y Y Y d D D D D D D D D D D D a A A A A A A A A A A A f F F F F F F F F F F F k K K K K K K K K K K K v V V V V V V V V V V V f F F F F F F F F F F F v V V V V V V V V V V V g G G G G G G G G G G G l L L L L L L L L L L L p P P P P P P P P P P P P T T T T T T T T T T t T T T T T T T T T T T g G G G G G G G G G G G . G G G G G G G G G G V V V V V V V P V V V A A A A A A A T A A P P P P P P P P P P S A A V A A A A A A A P P V V V A P P A P A A P P P A A A A . A A . . . P A I N . V V . . . A K A A A E E A A A P A A V A P P D D D G T V S A A S S S S E K E K K E E E E A A V A A V V V L K A A A A A A K K K K K Fig. 2 Homology profiles between the McPht and homologous Pht3 proteins. The pink colors indicate 75% amino acid similarity and blue colors indicate 100% amino acid similarity.