JIA-2018-09

2069 ZHANG Shuai et al. Journal of Integrative Agriculture 2018, 17(9): 2066–2073 Fig. 2 Outline of 5 biotypes of Aphis gossypii in northern China. Five biotypes are colored according to the neighbor-joining tree, which was constructed using 21 haplotypes and associated with its host plant. Table 1 Genetic diversity of 9 Aphis gossypii host-plant populations revealed by mitochondrial genes 1) Host plant Wing types 2) Collection date n Nh π (%) Hd Pomegranate Winged April, 2014 41 4 0.082 0.426 Wingless November, 2014 14 2 0.091 0.527 Winged November, 2014 4 2 0.087 0.500 Total 59 4 0.084 0.451 Hibiscus Wingless April, 2014 59 2 0.006 0.034 Wingless July, 2014 14 1 0.000 0.000 Wingless November, 2014 47 3 0.071 0.302 Winged November, 2014 14 2 0.046 0.264 Total 134 3 0.034 0.155 Chinese prickly ash Wingless April, 2014 44 6 0.204 0.686 Winged November, 2014 26 8 0.179 0.720 Wingless November, 2014 14 11 0.535 0.967 Total 84 20 0.339 0.800 Zucchini Wingless May, 2014 42 9 0.500 0.715 Winged May, 2014 48 4 0.444 0.537 Winged August, 2014 26 11 0.574 0.865 Winged September, 2014 43 9 0.380 0.590 Total 159 21 0.539 0.737 Cucumber Winged May, 2014 26 8 0.403 0.618 Wingless May, 2014 8 5 0.545 0.857 Winged August, 2014 3 3 0.924 1.000 Winged September, 2014 27 7 0.483 0.724 Total 64 16 0.566 0.795 Muskmelon Wingless May, 2014 24 4 0.343 0.486 Winged May, 2014 15 3 0.198 0.257 Total 39 5 0.290 0.401 Cotton Winged May, 2014 34 4 0.200 0.613 Wingless August, 2014 7 6 0.297 0.952 Winged September, 2014 29 5 0.081 0.369 Winged August, 2014 403 10 0.093 0.484 Total 473 18 0.114 0.511 Sophora japonica Wingless August, 2014 21 2 0.017 0.095 Kidney bean Winged May, 2014 10 5 0.385 0.800 1) Nh, number of haplotypes; π, nucleotide diversity; Hd, haplotype diversity. 2) All of the A . gossypii geographic populations were collected from Anyang, Henan Province except for the 19 populations from cotton fields in August, 2014, which was collected from the northern China (see details in Table 2).