JIA-2018-09

2049 ZHENG Na et al. Journal of Integrative Agriculture 2018, 17(9): 2042–2053 B C D H G F J K L P O N A E I M Fig. 6 The representative virulence phenotypes of the secretions or conidia of Fusarium spp. to soybean. Cultures of isolate #11 at the concentration of 1×10 7 conidia mL –1 were filtered to remove mycelia and gain conidia cultures, and then conidia cultures were centrifuged and separated into supernatants as the secretions and debris. The obtained debris was washed with sterilized ddH 2 O twice and centrifuged as the conidia, and then the conidia were suspended with appropriate volume of ddH 2 O as the conidia solution. A–H, PI 437654. I–P, Zhonghuang 13. A, E, I and M, CK. B, F, J and N, conidia cultures. C, G, K and O, supernatants. D, H, L and P, conidia. A–D and I–L, 0 day post infection. E–H and M–P, 5 days post infection. A B C D H G F E I J K L P O N M Fig. 7 Conidia of Fusarium solani isolate #4 alone underlies virulence to soybeans. Of the 17 isolated Fusarium spp., the isolate #4 at the concentration of 1×10 7 conidia mL –1 showed that the conidia caused the wilting of seedlings of PI 437654 and Zhonghuang 13, whereas the others did not. Cultures of isolate #4 at the concentration of 1×10 7 conidia mL –1 were filtered to remove mycelia and gain conidia cultures, and then conidia cultures were centrifuged and separated into supernatants as the secretions and debris. The obtained debris was washed with sterilized ddH 2 O twice and centrifuged as the conidia, and then the conidia were suspended with appropriate volume of ddH 2 O as the conidia solution. A–H, PI 437654. I–P, Zhonghuang 13. A, E, I and M, CK. B, F, J and N, conidia cultures. C, G, K and O, supernatants. D, H, L and P, conidia. A–D and I–L, 0 day post infection. E–H and M–P, 5 days post infection.