JIA-2018-09

Journal of Integrative Agriculture 2018, 17(9): 2042–2053 RESEARCH ARTICLE Available online at www.sciencedirect.com ScienceDirect Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted ZHENG Na 1* , ZHANG Liu-ping 1* , GE Feng-yong 1 , HUANG Wen-kun 1 , KONG Ling-an 1 , PENG De-liang 1 , LIU Shi-ming 1, 2 1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China 2 College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China Abstract Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans (virulent to one soybean whereas avirulent to another soybean), the others were all virulent to the two tested soybeans: both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×10 6 conidia mL –1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences (CAPS) markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean. Keywords: Fusarium , soybean root rot, conidia, secretions, virulence, cleaved amplified polymorphic sequences (CAPS) marker 1. Introduction Soybean ( Glycine max (L.) Merr.) is one important crop in the world, providing a sustainable source of protein and oil. During the growth and development, soybean is attacked by many kinds of pathogens worldwide causing huge yield losses annually as well as making soybean quality considerably affected. We developed an ethane methylsulfonate-mutagenesis population of soybean PI 437654 as the new genetic soybean resources (data not yet published). From this population, we found 11 M2 Received 13 December, 2017 Accepted 17 January, 2018 ZHENG Na, E-mail: 476211775@qq.com ; ZHANG Liu-ping, E-mail: Liupingz2013@163.com ; Correspondence LIU Shi-ming, E-mail: smliuhn@yahoo.com * These authors contributed equally to this study. © 2018 CAAS. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) doi: 10.1016/S2095-3119(17)61891-4