%A SONG Yang,LIU HongDi,WANG HaiBo,ZHANG HongJun,LIU FengZhi %T Molecular Cloning and Functional Characterization of VcNAC072 Reveals Its Involvement in Anthocyanin Accumulation in Blueberry %0 Journal Article %D 2019 %J Scientia Agricultura Sinica %R 10.3864/j.issn.0578-1752.2019.03.010 %P 503-511 %V 52 %N 3 %U {https://www.chinaagrisci.com/CN/abstract/article_20435.shtml} %8 2019-02-01 %X

【Objective】 The objective of this study was to isolate a transcription factor VcNAC072 (NAM, ATAF1/2, CUC2) from blueberry by PCR technology and studying its expression, and to identify its role in anthocyanin biosynthesis. This study laid the foundation for further study of the molecular mechanism of VcNAC072 affecting anthocyanin biosynthesis in blueberry. 【Method】 The blueberry VcNAC072 gene was cloned by PCR technology from the fruits of blueberry (Vaccinium corymbosum ‘Duke’). The transgenic Arabidopsis thaliana were generated via Agrobacterium-mediated transformation. The differences in the anthocyanin accumulation were compared between transgenic and wild-type Arabidopsis. The yeast one-hybrid (Y1H) and transient expression assays were carried out to test the transcriptional regulation of MYB transcription factor AtPAP1 by VcNAC072. 【Result】 A blueberry VcNAC072 was cloned from blueberry. Sequence analysis showed that the coding domain sequence (CDS) of VcNAC072 was 1 032 bp, which encoded 343 amino acids. Protein structure analysis showed that VcNAC072 contained a NAC domain. Expression analysis showed that VcNAC072 was expressed at different developmental stages of the blueberry fruits. However, the expression levels varied, with the highest expression level in pink fruits and blue fruits and the relatively low transcript levels in green fruits. The content of anthocyanin in fruits was increased with the elevation of relative expression of VcNAC072. The sequence of AtPAP1 promoter was analyzed and a NAC binding motif was found. The VcNAC072 protein could interact with the promoter of AtPAP1. Moreover, the VcNAC072 could induce the expression of AtPAP1 and favorably contributed to anthocyanin accumulation in seeds of transgenic Arabidopsis. 【Conclusion】 It was speculated that VcNAC072 up-regulated anthocyanin accumulation in fruits of blueberry.