Scientia Agricultura Sinica

Previous Articles    

Cuticle protein genes TcCP14.6 and TcLCPA3A are involved in phosphine resistance of Tribolium castaneum #br#

CHEH ErHu, MENG HongJie, CHEH Yan, TANG PeiAn   

  1. College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023
  • Published:2022-01-18

Abstract: ObjectiveCuticle proteins (CPs) are the main components of insect cuticle, and numerous studies have confirmed that the CP genes were involved in insecticide resistance. The present study was conducted to clarify the roles of TcCP14.6 (cuticle protein CP14.6) and TcLCPA3A (larval cuticle protein A3A) in phosphine resistance of Tribolium castaneum.MethodThe FAO-recommended bioassay method was used to determine the phosphine resistance levels of five different T. castaneum populations. The TcCP14.6 and TcLCPA3A sequences were downloaded from the T. castaneum genome data, and their encoded amino acid sequences, signal peptides and conserved domains were predicted via the online services. Additionally, the total RNAs were extracted from different tissues (the cuticles of head, chest and abdomen, wing, foot, gut, malpighian tubules and fat body), different phosphine resistance levels, as well as phosphine induction of T. castaneum respectively. Then, with TcRPS and TcRPL as internal reference genes, the RT-qPCR was used to analyze the expression patterns of TcCP14.6 and TcLCPA3A genes in different tissues, different phosphine resistance levels, and their expression profiles under phosphine induction. Lastly, the RNA interference (RNAi) technology was used to explore the relationship between two CP genes and phosphine resistance in T. castaneum. Result The bioassay analysis showed that Jiangsu (JS, RR=1.7) and Yunnan (YN, RR=3.0) belonged to susceptible populations, Hunan (HN, RR=20.2) belonged to moderately resistant population, Sichuan (SC, RR=395.4) and Guangdong (GD, RR=862.7) belonged to highly resistant populations. The sequence analysis demonstrated that both TcCP14.6 and TcLCPA3A proteins consisted of signal peptides and chitin binding domains. The RT-qPCR analysis suggested that TcCP14.6 and TcLCPA3A genes all had a higher expression in the peripheral tissues (the cuticles of head, chest and abdomen, wings and foot) of T. castaneum, and with a lower expression in the internal tissues, such as fat body, gut and malpighian tube. Besides, with the increase of phosphine resistance levels in T. castaneum, the TcCP14.6 increased and TcLCPA3A decreased their expression levels respectively. The expression levels of TcCP14.6 and TcLCPA3A genes were significantly increased and decreased respectively after phosphine induction for 6 h in T. castaneum. The injection of dsRNA could significantly inhibite the expression of TcCP14.6 and TcLCPA3A genes in phosphine resistance (GD) and susceptible (YN) populations of T. castaneum. When treated with phosphine (LC30), the insect mortality was significantly increased after the TcCP14.6 was silenced. Instead, the insect mortality was significantly decreased after the TcLCPA3A was knocked down.ConclusionThe two CP genes TcCP14.6 and TcLCPA3A are involved in phosphine resistance of T. castaneum.


Key words: Tribolium castaneum, phosphine resistance,  , cuticle , protein,  , TcCP14.6,  , TcLCPA3A, RNA interference

[1] CHEH ErHu, SHEN DanRong, DU WenWei, MENG HongJie, TANG PeiAn. Cuticle Protein Genes are Involved in Phosphine Resistance of Cryptolestes ferrugineus [J]. Scientia Agricultura Sinica, 2023, 56(9): 1696-1707.
[2] SHAO HongYang, MENG Xiang, ZHANG Tao, CHEN Min. Analysis of Cytochrome P450 Genes in Response to Quercetin and Function of CYP6ZB2 in Hyphantria cunea [J]. Scientia Agricultura Sinica, 2023, 56(7): 1322-1332.
[3] ZHANG Rui,ZHANG XueYao,ZHAO XiaoMing,MA EnBo,ZHANG JianZhen. Antibody Preparation and Subcellular Localization of LmKnk3-5′ in Locusta migratoria [J]. Scientia Agricultura Sinica, 2022, 55(2): 329-338.
[4] GUAN RuoBing,LI HaiChao,MIAO XueXia. Commercialization Status and Existing Problems of RNA Biopesticides [J]. Scientia Agricultura Sinica, 2022, 55(15): 2949-2960.
[5] YIN Fei,LI ZhenYu,SAMINA Shabbir,LIN QingSheng. Expression and Function Analysis of Cytochrome P450 Genes in Plutella xylostella with Different Chlorantraniliprole Resistance [J]. Scientia Agricultura Sinica, 2022, 55(13): 2562-2571.
[6] WU Wei,XU HuiLi,WANG ZhengLiang,YU XiaoPing. Cloning and Function Analysis of a Serine Protease Inhibitor Gene Nlserpin2 in Nilaparvata lugens [J]. Scientia Agricultura Sinica, 2022, 55(12): 2338-2346.
[7] CHEN ErHu,MENG HongJie,CHEN Yan,TANG PeiAn. Cuticle Protein Genes TcCP14.6 and TcLCPA3A are Involved in Phosphine Resistance of Tribolium castaneum [J]. Scientia Agricultura Sinica, 2022, 55(11): 2150-2160.
[8] Xiang XU,Yi XIE,LiYun SONG,LiLi SHEN,Ying LI,Yong WANG,MingHong LIU,DongYang LIU,XiaoYan WANG,CunXiao ZHAO,FengLong WANG,JinGuang YANG. Screening and Large-Scale Preparation of dsRNA for Highly Targeted Degradation of Tobacco Mosaic Virus (TMV) Nucleic Acids [J]. Scientia Agricultura Sinica, 2021, 54(6): 1143-1153.
[9] GE XinZhu,SHI YuXing,WANG ShaSha,LIU ZhiHui,CAI WenJie,ZHOU Min,WANG ShiGui,TANG Bin. Sequence Analysis of Harmonia axyridis Pyruvate Kinase Gene and Its Regulation of Trehalose Metabolism [J]. Scientia Agricultura Sinica, 2021, 54(23): 5021-5031.
[10] TAN YongAn,JIANG YiPing,ZHAO Jing,XIAO LiuBin. Expression Profile of G Protein-Coupled Receptor Kinase 2 Gene (AlGRK2) and Its Function in the Development of Apolygus lucorum [J]. Scientia Agricultura Sinica, 2021, 54(22): 4813-4825.
[11] YU WeiDong,PAN BiYing,QIU LingYu,HUANG Zhen,ZHOU Tai,YE Lin,TANG Bin,WANG ShiGui. The Structure Characteristics and Biological Functions on Regulating Trehalose Metabolism of Two NlTret1s in Nilaparvata lugens [J]. Scientia Agricultura Sinica, 2020, 53(23): 4802-4812.
[12] ZHANG DaoWei,KANG Kui,YU YaYa,KUANG FuPing,PAN BiYing,CHEN Jing,TANG Bin. Characteristics and Immune Response of Prophenoloxidase Genes in Sogatella furcifera [J]. Scientia Agricultura Sinica, 2020, 53(15): 3108-3119.
[13] LIU XiaoJian,GUO Jun,ZHANG XueYao,MA EnBo,ZHANG JianZhen. Molecular Characteristics and Function Analysis of Nuclear Receptor Gene LmE75 in Locusta migratoria [J]. Scientia Agricultura Sinica, 2020, 53(11): 2219-2231.
[14] YAO LiXiao,FAN HaiFang,ZHANG QingWen,HE YongRui,XU LanZhen,LEI TianGang,PENG AiHong,LI Qiang,ZOU XiuPing,CHEN ShanChun. Function of Citrus Bacterial Canker Resistance-Related Transcription Factor CitMYB20 [J]. Scientia Agricultura Sinica, 2020, 53(10): 1997-2008.
[15] MA Wen,LIU Jiao,ZHANG XueYao,SHEN GuoHua,QIN XueMei,ZHANG JianQin. Enzymatic Characteristics and Metabolic Analysis to Malathion and p,p’-DDT of LmGSTS2 from Locusta migratoria [J]. Scientia Agricultura Sinica, 2019, 52(8): 1389-1399.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!