Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (6): 1256-1262.doi: 10.3864/j.issn.0578-1752.2013.06.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Preparation and Characterization of Ractopamine-Imprinted Material Using Surface-Molecular Imprinting Method and Its Adsorption Characteristics

 YANG  Ting, WU  Yin-Liang, LI  Cun, ZHAO  Jian, CHEN  Guo, LU Yan   

  1. 1.Ningbo Academy of Agricultural Science, Ningbo 315040, Zhejiang
    2.Tianjin Agriculture University, Tianjin 300384
  • Received:2012-07-23 Online:2013-03-15 Published:2013-01-18

Abstract: 【Objective】 The objective of this experiment is to prepare a molecule-imprinted material on surface of silica gel for ractopamine. 【Method】 Polymethylacrylic acid (PMAA) was grafted onto the surface of silica gel particles via the coupling effect of γ-methacryloxypropyl trimethoxy silane, and the grafting particle PMAA/SiO2 was prepared. Then, the molecular imprinting towards the grafted PMAA was performed with ractopamine as template molecule and ethylene glycol diglycidylether (EGDE) as crosslinking agent by using the surface-molecular imprinting technique established, and the ractopamine molecule- imprinted material MIP-PMAA/SiO2 was prepared. The morphology of MIP-PMAA/SiO2 was observed by SEM,and the chemical structure of MIP-PMAA/SiO2 was characterized by FT-IR. Static method was adopted to study the binding properties and molecule recognition characters of MIP-PMAA/SiO2 for ractopamine.【Result】The MIP-PMAA/SiO2 on the adsorption ability of ractopamine was significantly better than clenbuterol and salbutamol. The adsorption capacity of the MIP-PMAA/SiO2 was 11.08 mg•g-1, the specificity binding capacity was 9.93 mg•g-1, and the imprinted factor of the polymer was 9.63. The adsorption reaction reached equilibrium after 10 min, and the desorption ration of ractopamine reached 92.3% in 5 min.【Conclusion】The experimental results show that MIP-PMAA/SiO2 have specific recognition selectivity, excellent binding affinity and elution property for ractopamine.

Key words: surface molecular imprinting , ractopamine , characterization , adsorption characteristics

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