中国农业科学 ›› 2013, Vol. 46 ›› Issue (8): 1668-1675.doi: 10.3864/j.issn.0578-1752.2013.08.016

• 园艺 • 上一篇    下一篇

三个品种茶树氟富集特性及其在亚细胞中的分布

 蔡荟梅, 彭传燚, 李成林, 高柱, 侯如燕, 宛晓春   

  1. 1.安徽农业大学茶与食品科技学院/教育部农业部茶叶生物化学与生物技术重点实验室,合肥 230036
    2.安徽绿魁茶业有限公司,安徽宣城 242131
    3.国家农副加工食品质量监督检验中心,合肥 230001
  • 收稿日期:2012-08-17 出版日期:2013-04-15 发布日期:2013-01-23
  • 通讯作者: 蔡荟梅,E-mail:hml20@sina.com
  • 作者简介:蔡荟梅,E-mail:hml20@sina.com
  • 基金资助:

    国家现代农业(茶)产业技术体系(农科教发[2008]10号)、安徽省教育厅重点项目(KJ2011A105)

Fluoride Accumulation and Its Subcellular Distribution in Three Tea Plants

 CAI  Hui-Mei, PENG  Chuan-Yi, LI  Cheng-Lin, GAO  Zhu, HOU  Ru-Yan, WAN  Xiao-Chun   

  1. 1.College of Tea&Food Science and Technology, Anhui Agricultural University/Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education & ministry of Agriculture, Anhui Agricultural University, Hefei 230036
    2.Anhui Lükui tea industry Co.,   Ltd, Xuancheng,242131, Anhui
    3.China National Center for Quality Supervision and Test of Agricultural-Avocation        Processed Food, Hefei 230001
  • Received:2012-08-17 Online:2013-04-15 Published:2013-01-23

摘要: 【目的】研究茶树对氟吸收、富集规律及其解毒机理。【方法】通过水培试验,结合差速离心方法研究平阳特早、乌牛早和凫早2号氟富集的特性及根和叶片中氟的亚细胞分布,比较不同品种之间的差异。【结果】3个品种茶树根、茎和叶氟含量均随着培养介质氟浓度的增加而升高,0—10 mg•L-1氟处理下,地上部分氟含量大于根部氟含量(转运系数>1),且茶树吸收的氟中56%贮存在地上部分。除对照组外,大部分氟积累在叶片和根系的细胞壁(76.84%—91.58%)和可溶性部分(53.24%—80.35%),氟的添加均增加了氟在3个品种茶树细胞壁和可溶性部分中的分配比例,减少了其在细胞器中的富集。加氟处理下,平阳特早和乌牛早叶片亚细胞组分氟含量的变化趋势为:细胞壁>可溶性部分>细胞器;凫早2号为可溶性部分>细胞壁>细胞器。低氟(0—5 mg•L-1)氟处理下,茶树根系亚细胞氟含量变化趋势为:细胞器≥细胞壁>可溶性部分;高氟(10—50 mg•L-1)氟处理下,则为细胞壁>可溶性部分≥细胞器。【结论】转运系数、地上部分氟的贮存率和氟在亚细胞组分的分布表明,茶树具有较强的向上运输氟的能力;氟在茶树根和叶中的亚细胞分布与茶树的品种和溶液氟处理的浓度有关,叶片中总氟含量与各亚细胞组分氟含量之间及其各亚细胞组分氟含量之间的关系与品种有关,根系表现与品种无关;细胞壁和可溶性部分是茶树高氟处理下氟主要的存储部位,说明细胞壁沉淀和细胞内的区隔化作用可能是茶树能够解除氟毒的重要原因。

关键词: 茶树品种 , 氟 , 富集 , 亚细胞分布

Abstract: 【Objective】The objective of this study was to give some experimental basis for studying the absorption, accumulation of F and the mechanism of detoxification.【Method】Solution culture and differential centrifugation were conducted to study the characteristics of F accumulation and its subcellular distribution of Pingyangtezao, Wuniuzao and Fuzao No.2, with comparison of the differences between varieties.【Result】F concentration in roots, stems and leaves of three kinds of tea plants increased with F treatment, under 0-10 mg•L-1 F treatments, the ground part of fluoride content was greater than the root(Translation correlation >1) and 56% of F was distributed in above-ground part. Except for the control, most of the fluoride was distributed in cell wall (76.84%-91.58%) and soluble fraction (53.24%-80.35%) of leaf and root. F treatment generally increased the percentage of cell wall and soluble fraction, but decreased that of cell organelle. In F treatment, the change tendency of F concentration of subcellular fractions of leaf of Pingyangtezao and Wuniuzao ranked in the following order: cell wall> soluble fraction> cell organelle, while soluble fraction>cell wall> cell organelle for Fuzao No.2 . In low F (0-5 mg•L-1 ) treatment , the change tendency of F concentration of subcellular fractions of root of three kinds of tea plants ranked in the following order: cell organelle≥cell wall>soluble fraction, while cell wall>soluble fraction≥cell organelle in high F (10-50 mg•L-1 ) treatment.【Conclusion】Translation correlation , percentage of F for above-ground part and F subcellular distribution in root and leaf suggested tea plant was of strong upward transportation capacity. The subcellular distribution of F in leaves and roots were related with tea species and solution F treatment, and so did the correlation between the concentration of T-F and subcellular fractions in leaf, with some differences, but no in root. Cell wall and soluble fraction were the main storage sites in high F treatment, suggesting cell wall binding and vacuolar compartmentalization may be main reasons for tea plant to detoxify fluoride toxicity.

Key words: tea varieties , fluoride , accumulation , subcellular distribution