转Bt基因水稻的碳代谢特性

doi:10.3864/j.issn.0578-1752.2013.08.005

摘要/Abstract

摘要： 【目的】评价外源Bt基因导入对水稻碳代谢特性的影响。【方法】以转Bt基因水稻克螟稻1号及其非转基因亲本秀水11，以及克螟稻1号与3个杂交水稻恢复系明恢63、R3027和99亚162杂交和连续回交而育成的农艺性状和抗虫性稳定品系的3对近等基因系为材料，对叶绿素与可溶性糖含量、RuBP 羧化酶、蔗糖合成酶及蔗糖磷酸合成酶活性、干物质及有机碳积累量等碳代谢指标进行了测定。【结果】分蘖盛期克螟稻1号的叶绿素a含量、可溶性糖含量、干物质和有机碳含量均极显著低于秀水11（P＜0.01），叶绿素b含量、蔗糖合成酶和蔗糖磷酸合成酶活性显著低于秀水11（P＜0.05），但其它3对Bt水稻与非Bt水稻间所有生理指标均无显著差异；齐穗期克螟稻1号蔗糖磷酸合成酶活性显著高于秀水11，而干物质和有机碳积累量则显著低于秀水11；BtR3027蔗糖合成酶活性显著低于R3027，而Bt99亚162可溶性糖含量和蔗糖磷酸合成酶活性则显著高于99亚162；成熟期所有Bt水稻与各自非Bt水稻间干物质重和有机碳积累量差异均不显著。【结论】克螟稻1号大多数碳代谢指标的显著变化主要是由无性系变异引起的；3对近等基因系中Bt基因对个别碳代谢指标的影响是短暂的，且与Bt基因所处的遗传背景有关。

关键词: 水稻 , Bt基因 , 碳代谢 , 生理指标

Abstract: 【Objective】The objective of this study was to evaluate the effect of transgene Cry1Ab on carbon metabolism characteristics in rice. 【Method】Transgenic Bt (Cry1Ab) rice KMD1 and its parent Xiushui 11, as well as three pairs of Bt and non-Bt rice near-isogenic lines which were derived from the progeny of crosses and continuous backcrosses between the KMD1 and Minghui 63, R3027 and 99Ya162, respectively, were field grown and measured for the content of chlorophyll and soluble sugar, the activity of RuBPCase, sucrose synthetase and sucrose phosphate synthase in leaves, the dry weight and organic carbon accumulation per plant at different developmental stages.【Result】At tillering stage, KMD 1 had significant lower contents of chlorophyll a, chlorophyll b and soluble sugar, lower activities of sucrose synthetase and sucrose phosphate synthase, and lower dry weight and organic carbon accumulation than those of Xiushui 11, but no significant differences between other 3 pairs of Bt and non-Bt rice lines were observed in all physiological indexes tested. At full heading stage, the activity of sucrose phosphate synthase of KMD1 was significantly higher than that of Xiushui 11, although its dry weight and organic carbon accumulation were significantly lower, while Bt R3027 showed a significant lower sucrose synthetase activity and Bt 99Ya162 showed significant higher soluble sugar content and sucrose phosphate synthase activity than the non-Bt type, respectively. At maturity stage, no significant differences were found in dry weight and organic carbon accumulation in four pairs of Bt and non-Bt rice lines. 【Conclusion】Significant change of most physiological indexes related to carbon metabolism in transgenic rice KMD 1 was resulted from somaclonal variations, while the effect of Cry1Ab gene on individual index in other 3 pairs of Bt and non-Bt rice near-isogenic lines was transitory and related to the genetic background of Bt gene incorporation.

Key words: rice , Bt gene , carbon metabolism , physiological indexes

王伟博, 崔海瑞, 卢美贞, 舒庆尧, 沈圣泉. 转Bt基因水稻的碳代谢特性[J]. 中国农业科学, 2013, 46(8): 1564-1570.

WANG Wei-Bo, CUI Hai-Rui, LU Mei-Zhen, SHU Qing-Yao, SHEN Sheng-Quan. Research on Carbon Metabolism Characteristics of Transgenic Bt Rice[J]. Scientia Agricultura Sinica, 2013, 46(8): 1564-1570.

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参考文献

[1]舒庆尧, 叶恭银, 崔海瑞, 项友斌, 高明尉. Bt转基因抗虫水稻“克螟稻”的培育. 浙江农业大学学报, 1998, 24(6): 579-580.

Shu Q Y, Ye G Y, Cui H R, Xiang Y B, Gao M W. Development of transgenic Bacillus thuriengiensis rice resistant to rice stem borers and leaf folders. Journal of Zhejiang Agricultural University, 1998, 24(6): 579-580. (in Chinese)

[2]Shu Q Y, Ye G Y, Cui H R, Cheng X Y, Xiang Y B, Wu D X, Gao M W, Xia Y W, Hu C, Sardana R, Altosaar I. Transgenic rice plants with a synthetic crylAb gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species. Molecular Breeding, 2000, 6: 433-439.

[3]Tu J, Zhang G, Datta K, Xu C, He Y, Zhang Q, Khush G S, Datta S K. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin. Nature Biotechnology, 2000, 18(10): 1101-1104.

[4]朱桢. 高效抗虫转基因水稻的研究与开发. 中国科学院院刊，2001(5): 353-357.

Zhu Z. Research and development of highly insect-resistant transgenic rice. Bulletin of Chinese Academy of Sciences, 2001(5): 353-357. (in Chinese)

[5]张立军, 刘新. 植物生理学, 第二版. 北京: 科学出版社, 2011: 96-148.

Zhang L J, Liu X. Plant Physiology, Second Edition. Beijing: Science Press, 2011: 96-148. (in Chinese)

[6]林瑞余, 蔡碧琼, 柯庆明, 蔡向阳, 林文雄. 不同水稻品种产量形成过程的固碳特性研究. 中国农业科学, 2006, 39(12): 2441-2448.

Lin R Y, Cai B Q, Ke Q M, Cai X Y, Lin W X. Characteristics of carbon fixation in different rice cultivars during yield formation process. Scientia Agricultura Sinica, 2006, 39(12): 2441-2448. (in Chinese)

[7]李亚娟, 陈志雄, 粱康迳, 林文雄, 梁义元. 水稻籽粒碳氮代谢与品质性状问遗传相关性的研究. 中国生态农业学报, 2006, 14(3): 176-179.

Li Y J, Chen Z X, Liang K J, Lin W X, Liang Y Y. Genetic correlation between the metabolism of carbohydrate and nitrogen and the main qualities of grain in rice (Oryza sativa L)．Chinese Journal of Eco-Agriculture, 2006, 14(3): 176-179. (in Chinese)

[8]童平, 杨世民, 马均, 吴合洲, 傅泰露, 李敏, 王明田. 不同水稻品种在不同光照条件下的光合特性及干物质积累. 应用生态学报, 2008, 19(3): 505-511.

Tong P, Yang S M, Ma J, Wu H Z, Fu T L, Li M, Wang M T. Photosynthetic characteristics and dry matter accumulation of hybrid rice varieties under different light conditions. Chinese Journal of Applied Ecology, 2008, 19(3): 505-511. (in Chinese)

[9]De Costa W A J M, Weerakoon W M W, Herath H M L K, Abeywardena R M I. Response of growth and yield of rice (Oryza sativa) to elevated atmospheric carbon dioxide in the sub-humid zone of Sri Lanka. Journal of Agronomy and Crop Science, 2003, 189: 83-95.

[10]Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Wang W. Remobilization of carbon reserves in response to water deficit during grain filling of rice. Field Crops Research, 2001, 71(1): 47-55.

[11]Shu Q Y, Cui H R, Ye G Y, Wu D X, Xia Y W, Gao M W, Altosaar I. Agronomic and morphological characterization of Agrobacterium- transformed Bt rice plants. Euphytica, 2002, 127: 345-352.

[12]贾乾涛, 石尚柏, 杨长举, 彭于发. 转Bt基因水稻生长期几种重要成分含量的变化研究. 中国农业科学, 2005, 38(10): 2002-2006.

Jia Q T, Shi S B, Yang C J, Peng Y F. Changes of several important materials in transgenic Bt rice. Scientia Agricultura Sinica, 2005, 38(10): 2002-2006. (in Chinese)

[13]刘微, 王树涛, 陈英旭, 吴伟祥. 转Bt(Cry1Ab)基因对水稻光合特性及光合产物积累的影响. 中国农业科学, 2011, 44(3): 627-633.

Liu W, Wang S T, Chen Y X, Wu W X. Effect of Cry1Ab gene on photosynthetic characteristics and photosynthate accumulation of rice. Scientia Agricultura Sinica, 2011, 44(3): 627-633. (in Chinese)

[14]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 1994, 144: 307-313.

[15]中国科学院上海植物生理研究所. 现代植物生理学实验指南. 北京: 科学出版社, 1999: 113-116.

Shanghai Research Institute of Plant Physiology, The Shanghai Society for Plant Physiology eds. Modern Plant Physiological Experiment Manual. Beijing: Science Press, 1999: 113-116. (in Chinese)

[16]Kruger N J. The Bradford method for protein quantitation. Methods in Molecular Biology, 1994, 32: 9-15.

[17]张志良, 瞿伟菁. 植物生理学实验指导, 第三版. 北京: 高等教育出版社, 2003: 127-128, 137-138.

Zhang Z L, Qu W J. The Experimental Guide for Plant Physiology, Third Edition. Beijing: Higher Education Press, 2003: 127-128, 137-138. (in Chinese)

[18]吴良欢, 陶勤南. 植物有机碳改进测定法研究. 土壤通报, 1993, 24(6): 286-287.

Wu L H, Tao Q N. Study of improved mensuration of plant organic carbon. Chinese Journal of Soil Science, 1993, 24(6): 286-287. (in Chinese)

[19]Martin C, Thimann K V. The role of protein synthesis in the senescence of leaves. Journal of Plant Physiology, 1972, 49:64-71.

[20]Anderson J M, Park Y L, Chow S W. Photoinactivation and photoprotection of photosystem II in nature. Physiologia Plantarum, 1997, 100: 214-223.

[21]翁晓燕, 陆庆, 蒋德安. 水稻Rubisco活化酶在调节Rubisco活性和光合日变化中的作用. 中国水稻科学, 2001, 15(1): 35-40.

Weng X Y, Lu Q, Jiang D A. Rubisco activase and its regulation on diurnal changes of photosynthetic rate and the activity of ribulose 1, 5-bisphosphate carboxyase /oxygenase (rubisco). Chinese Journal of Rice Science, 2001, 15(1): 35-40. (in Chinese)

[22]王忠华, 陈小坚, 包旭升, 陈玉玲, 顾勤琴. 辐照处理对Bt水稻苗期生理生化特性的影响. 核农学报, 2011, 25(1): 174-178.

Wang Z H, Chen X J, Bao X S, Chen Y L, Gu Q Q. Effect of irradiation on physiological and biochemical properties of Bt rice seedlings. Journal of Nuclear Agriculture Sciences, 2011, 25(1): 174-178. (in Chinese)

[23]卢合全, 沈法富, 刘凌霄, 孙维方. 植物蔗糖合成酶功能与分子生物学研究进展. 中国农学通报, 2005, 7(21): 34-37.

Lu H Q, Shen F F, Liu L X, Sun W F. Recent advances in study on plant sucrose synthase. Chinese Agriculture Science Bulletin, 2005, 7(21): 34-37. (in Chinese)

[24]Larkin P J, Scowcroft W R. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 1981, 60: 197-214.

[25]Bairu M W, Aremu A O, Johannes V S. Somaclonal variation in plants: causes and detection methods. Plant Growth Regulation, 2011, 63: 147-173.

[26]崔海瑞, 王忠华, 舒庆尧, 吴殿星, 夏英武, 高明尉. 转Bt基因水稻克螟稻杂交转育后代农艺性状的研究. 中国水稻科学, 2001, 15(2) : 101-106.

Cui H R, Wang Z H, Shu Q Y, Wu D X, Xia Y W, Gao M W. Agronomic traits of hybrid progenies between Bt transgenic rice and conventional rice varieties. Chinese Journal of Rice Science, 2001, 15(2): 101-106. (in Chinese)