遮光下外源水杨酸对韭菜硝酸盐还原同化效应的研究

doi:10.3864/j.issn.0578-1752.2012.20.011

摘要/Abstract

摘要： 【目的】通过研究不同光强下外源水杨酸（SA）对韭菜叶片硝酸盐（NO3-）累积、氮代谢关键酶活性、光系统II电子传递速率（ETR）及主要氨基酸和可溶性蛋白合成的调控效应，以期明确遮光水平下SA对硝酸盐还原同化的影响。【方法】以韭菜品种‘新世纪雪韭’为供试材料，在前期试验工作基础上，采用显著降低韭菜硝酸盐累积的3.0 mmol•L-1 SA对韭菜叶面进行喷施处理，设自然光（900—1 050 μmol•m-2•s-1）和遮光（40%自然光）2种光照强度，分析SA对韭菜硝酸盐累积及NO3-还原同化的影响。【结果】SA处理缓解了遮光水平下韭菜叶片氮代谢关键酶硝酸还原酶（NR）、谷氨酰胺合成酶（GS）、谷氨酸草酰乙酸转氨酶(GOT)和谷氨酸丙酮酸转氨酶（GPT）活性的降低，显著降低了硝酸盐的累积，其中，与弱光对照相比，SA处理下GOT活性和GPT增幅较明显，分别为23.7%和12.3%；SA处理还显著提高了弱光下叶片的全氮含量和干物率，但叶绿素含量和光系统间电子传递速率ETR的增幅不大；另外，SA处理增加了弱光下韭菜叶片多数游离氨基酸组分的含量，效果最为显著的为色氨酸和丝氨酸，分别比对照提高了89.8%和50.6%；并且提高了游离氨基酸总量和可溶性蛋白含量，但降低了游离氨基酸与可溶性蛋白比值A/P。【结论】弱光降低了韭菜氮素同化能力和物质生产能力，而SA处理促进了弱光下韭菜叶片氮代谢中硝酸盐的还原和铵的同化，同时调动转氨作用的积极协同配合，促进了硝态氮转化为游离氨基酸和可溶性蛋白，这可能是遮光下SA降低韭菜硝酸盐累积的一个重要原因。

关键词: 水杨酸, 遮光, 韭菜, 硝酸盐还原同化, 氮代谢

Abstract: 【Objective】The effect of exogenous salicylic acid (SA) on accumulation of nitrate, the key enzyme activity of nitrogen metabolism, the apparent electron transport rate (ETR) and the main amino acids and soluble protein was studied in Chinese chive under the weak light in order to understand the effects of exogenous salicylic acid on nitrate reduction and assimilation.【Method】With Chinese chive as an experimental material, two treatment factors including SA (3.0 mmol•L-1) and light intensity (the weak light and natural light) were designed in the experiment. 【Result】Under the weak light, exogenous SA pretreatment reduced the decrease in the key enzyme activity of nitrogen metabolism (nitrate reductase (NR), glufamine synthetase (GS), glutamic- oxaloacetic-transaminase (GOT) and glutamate-pyruvate-transaminase (GPT)), while decreased nitrate accumulation. Compared with no SA treatments，NR and GS activities in SA treatments increased significantly , 23.7% and 12.3%, respectively. Also, the content of chlorophyll (Chl), ETR , the content of free amino acids and soluble protein of weak light were enhanced after SA application. In addition, SA increased the content of most free amino acid components under weak light, with tryptophan and serine as the most effective, reaching 89.8% and 50.6%, respectively, and at the same time reduced the free amino acids and soluble protein ratio (A/P). 【Conclusion】Weak light reduced the capacity of the nitrogen assimilation and material production, however, exogenous SA spraying on leaves obviously improved nitrate reduction and assimilation, which, meanwhile, brought transaminations into play vigorously, and promoted turning NO3- into free amino acid and dissolvable protein. Improvement of nitrate reduction and assimilation might be a key reason for reducing nitrate accumulating into the vacuole of Chinese chive under weak light.

Key words: salicylic acid, shading, Chinese chive, nitrate reduction and assimilation, nitrogen metabolism

李林妍, 王俊玲, 王梅, 高志奎. 遮光下外源水杨酸对韭菜硝酸盐还原同化效应的研究[J]. 中国农业科学, 2012, 45(20): 4216-4223.

LI Lin-Yan, WANG Jun-Ling, WANG Mei, GAO Zhi-Kui. Effects of Exogenous Salicylic Acid on Nitrate Reduction and Assimilation in Chinese Chive Under Weak Light[J]. Scientia Agricultura Sinica, 2012, 45(20): 4216-4223.

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参考文献

[1]高秀瑞，陈凤敏，刁春英，潘秀清，武彦荣，李　冰，陈贵林. 不同形态氮素替代硝态氮对蔬菜硝酸盐含量变化的影响. 华北农学报，2008, 23(6): 208-211.

Gao X R, Chen F M, Diao C Y, Pan X Q, Wu Y R, Li B, Chen G L. Effect of partial replacement of nitrate in solution by amino acid and urea on accumulation of nitrate in non-heading Chinese cabbage and lettuce. Acta Agriculturae Boreali-Sinica, 2008, 23(6): 208-211. ( in Chinese)

[2]宋艳霞，杨文钰，李卓玺，雍太明，刘岚. 套作遮荫对大豆不同品种苗期氮代谢的影响. 中国油料作物学报, 2010, 32(3): 390-394.

Song Y X, Yang W Y, Li Z X, Yong T M, Liu L. Effect of maize-soybean relay cropping shade on nitrogen metabolism of soybean seedlings. Chinese Journal of Oil Crop Sciences, 2010, 32(3): 390-394. ( in Chinese)

[3]Hecht U, Oelmüller R, Schmidt S, Mohr H. Action of light, nitrate and ammonium on the levels of NADH and ferredoxin-dependent glutamate synthases in the cotyledons of mustard seedlings. Planta, 1988, 175: 130-138.

[4]于莎，王友华，周治国，吕丰娟，刘敬然，马伊娜，陈吉，阿布都克尤木•阿不都热孜克. 花铃期遮阴对棉花氮素代谢的影响及其机制. 作物学报, 2011, 37(10): 1879-1887.

Yu S, Wang Y H, Zhou Z G, Lü F J, Liu J R, Ma Y N, Chen J, Abudukeyoumu A. Effect of shade on nitrogen metabolism and its mechanism in cotton plant at flowering and boll-forming stage. Acta Agronomica Sinica, 2011, 37(10): 1879-1887. ( in Chinese)

[5]孟雪娇，邸昆，丁国华. 水杨酸在植物体内的生理作用研究进展. 中国农学通报, 2010, 26(15): 207-214.

Meng X J, Di K, Ding G H. Progress of study on the physiological role of salicylic acid in plant. Chinese Agricultural Science Bulletin, 2010, 26(15): 207-214. ( in Chinese)

[6]Belinda A, Barbaros N. Effects of cold and salicylic acid treatments on nitrate reductase activity in spinach leaves, Turkish Journal of Biology, 2011, 35: 443-448.

[7]曹岩坡，高志奎，何俊萍，王梅，高荣孚. 外源水杨酸对韭菜硝酸盐还原与同化的影响. 园艺学报, 2009, 36(3): 415-420.

Cao Y P, Gao Z K, He J P, Wang M, Gao R F. Effects of exogenous salicylic acid on nitrate accumulation and reduction and assimilation in the leaves of chinese chive. Acta Horticulturae Sinica, 2009, 36(3): 415-420. ( in Chinese)

[8]Singh B, Usha K. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 2003, 39: 137-141.

[9]Jain A, Srivastava H S. Effect of salicylic acid on nitrate reductase activity in maize seedlings. Plant Physiology, 1981, 51:339.

[10]刘伟，艾希珍，梁文娟，王洪涛，刘升学，郑楠．低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响．应用生态学报, 2009, 20(2): 441-445．

Liu W, Ai X Z, Liang W J, Wang H T, Liu S X, Zheng N. Effects of salicylic acid on the leaf photosynthesis and antioxidant enzyme activities of cucumber seedlings under low temperature and light intensity. Chinese Journal of Applied Ecology, 2009, 20(2): 441-445．( in Chinese)

[11]霍捷，高志奎，王俊玲，赵飞. 高温胁迫下外源水杨酸对韭菜硝酸盐累积及PSⅡ光能吸收利用的影响. 中国农学通报，2011, 27(6): 133-137.

Huo J, Gao Z K, Wang J L, Zhao F. Effects of exogenous salicylic acid on nitrate accumulation and light absorption and utilization of photosystemⅡin the leaves of chinese chive under high temperature stress. Chinese Agricultural Science Bulletin, 2011, 27(6): 133-137. ( in Chinese)

[12]郝敬虹，易旸，尚庆茂，董春娟，张志刚. 水杨酸处理对干旱胁迫下黄瓜幼苗氮素同化及其关键酶活性的影响. 园艺学报，2012, 39(1): 81-90.

Hao J H, Yi Y, Shang Q M, Dong C J, Zhang Z G. Effect of exogenous salicylic acid on nitrogen assimilation of cucumber seedling under drought stress. Acta Horticulturae Sinica, 2012, 39(1): 81-90. ( in Chinese)

[13]孙春杰，徐杰，刘成连，原永兵. 水杨酸诱导黄瓜幼苗抗冷性的基因表达. 中国农学通报, 2009, 25(10): 178-182.

Sun C J, Xu J, Liu C L, Yuan Y B. Gene expression of chilling tolerance induced by salicylic acid in cucumber seedling. Chinese Agricultural Science Bulletin, 2009, 25(10): 178-182. (in Chinese)

[14]张以顺，黄霞，陈云凤. 植物生理学试验教程. 北京: 高等教育出版社, 2009.

Zhang Y S, Huang X, Chen Y F. Plant Physiology Experiment Tutorial. Beijing: Higher Education Press, 2009. (in Chinese)

[15]吴良欢，蒋式洪，陶勤南. 植物转氨酶(GOT和GPT)活度比色测定方法及其应用. 土壤通报, 1998, 29(3): 136-138.

Wu L H, Jiang S H, Tao Q N. Plant aminotransferase (GOT and GPT) activity the colorimetric determination method and its application. Chinese Journal of Soil Science, 1998, 29(3): 136-138. (in Chinese)

[16]Mackown C T, Jakson W A, Volk R G. Partioning of previously accumulated nitrate to translocation, reduction, and efflux in corn roots. Planta, 1983, 157: 8-14.

[17]卢凤刚，陈贵林，吕桂云，任良玉. 不同供氮水平对韭菜产量和品质的影响. 园艺学报, 2005, 32(1): 131-133.

Lu F G, Chen G L, Lü G Y, Ren L Y. Effects of different nitrogen concentrations on yield and quality of Chinese chive. Acta Horticulturae Sinica, 2005, 32(1): 131-133. ( in Chinese)

[18]王强，钟旭华，黄农荣，郑海波. 光、氮及其互作对作物碳氮代谢的影响研究进展. 广东农业科学, 2006(2): 37-40.

Wang Q, Zhong X H, Huang N R, Zheng H B. Interactions of nitrogen with light in the photosynthetic traits and metabolism of carbon and nitrogen of crop. Guangdong Agricultural Sciences, 2006 (2): 37-40. ( in Chinese)

[19]Bowsher C G, Long D M, Oaks A, Rothstein S J. Effect of light/dark cycles on expression of nitrate assimilatory genes in maize shoots and roots. Journal of Plant Physiology, 1991, 95: 281-285.

[20]泰兹(美), 奇格尔(美). 宋纯鹏,王学路等译. 植物生理学. 第四版. 北京: 科学出版社, 2009: 236-243.

Taiz L, Zeiger E. Translated by Song C P, Wang X L, et al. Plant Physiology. Fourth edition. Beijing: Science Press, 2009: 236-243. (in Chinese)

[21]沈国栋. 水杨酸调节怀槐培养细胞合成异黄酮与碳氮代谢的关系研究[D]. 合肥: 合肥工业大学, 2004.

Shen G D. Study on the relationship between isoflavone synthesis and carbon- and nitrogen-metabolism regulated by salicylic acid in cultured cells of Maackia amurensis[D]. Hefei: Hefei University of Technology, 2004. (in Chinese)

[22]El-Tayeb M A. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 2005, 45(3): 215-224.

[23]Ananiera E A, Alexieva V S, Popova L. Treatment with salicylic acid decreases the effects of paraquat on photosynthesis. Journal of Plant Physiology, 2002, 159: 685-693.

[24]王利军，李家承，刘允芬，刘琪瑾，黄卫东，石玉林. 高温干旱胁迫下水杨酸和钙对柑橘光合作用和叶绿素荧光的影响. 中国农学通报, 2003, 19(6): 185-189.

Wang L J, Li J C, Liu Y F, Liu Q J, Huang W D, Shi Y L. Effects of salicylic acid and CaCl2 on photosynthesis and chlorophyll fluorescence of citrus under heat and drought stress. Chinese Agricultural Science Bulletin, 2003, 19(6): 185-189. ( in Chinese)

[25]黄清泉，孙歆，张年辉，冯鸿，袁澍，代其林，梁厚果，杜林方，林宏辉．水杨酸对水分胁迫黄瓜幼苗叶片生理过程的影响．西北植物学报, 2004, 24(12): 2202-2207．

Huang Q Q, Sun X, Zhang N H, Feng H, Yuan S, Dai Q L, Liang H G, Du L F, Lin H H. Effects of salicylic acid on leaves of cucumber seedlings under water stress. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(12): 2202-2207. (in Chinese)

[26]陈仕江，钟国跃，徐金辉. 黄连生育期间可溶性糖和氨基酸含量动态的研究. 中国中药杂志, 2005, 30(17): 1324-1327.

Chen S J, Zhong G Y, Xu J H. Dynamic change of soluble saccharides and amino acids during the growing period of Coptis chinensis. China Journal of Chinese Material Medicine, 2005, 30(17): 1324-1327. ( in Chinese)

[27]陈贵林，高秀瑞. 氨基酸和尿素替代硝态氮对水培不结球白菜和生菜硝酸盐含量的影响. 中国农业科学, 2002, 35(2): 187-191.

Chen G L, Gao X R. Effect of partial replacement of nitrate by amino acid and urea on nitrate content of nonheading Chinese cabbage and lettuce in hydroponics. Scientia Agricultura Sinica, 2002, 35(2): 187-191. (in Chinese)

[28]Miller A J, Fan X R, Shen Q R, Smith S J. Amino acids and nitrate as signals for the regulation of nitrogen acquisition. Journal of Experimental Botany, 2008, 59(1): 111-119.

[29]周诗毅，陈晓菁，黄建国，何光源. 八种氨基酸对水稻硝酸盐吸收的影响. 生物技术, 2009, 19(4): 58-60.

Zhou S Y, Chen X Q, Huang J G, He G Y. Effects of eight amino acids on the nitrate uptake in rice. Biological Technology, 2009, 19(4): 58-60. ( in Chinese)

[30]Migge A, Bork C, Hell R, Becker T W. Negative regulation of nitrate reductase gene expression by glutamine or as paragine accumulating in leaves of sulfur-deprived tobacco. Planta, 2000, 211: 587-595.

[31]彭新湘，李明启. 光呼吸代谢物乙醇酸、乙醛酸和草酸对烟草叶片硝酸还原的影响. 植物生理学报, 1987, 13(2): 182-189.

Peng X X, Li M Q. Effects of photorespiratory metabolites glycolate, glyoxylate and oxalate on nitrate reduction in tobacco leaf. Acta Phytophysiologica Sinica, 1987, 13(2): 182-189. (in Chinese)