高效液相色谱法同时测定柑橘果实中18种类黄酮的含量

doi:10.3864/j.issn.0578-1752.2012.17.012

中国农业科学 ›› 2012, Vol. 45 ›› Issue (17): 3558-3565.doi: 10.3864/j.issn.0578-1752.2012.17.012

• 园艺·贮藏·保鲜·加工 • 上一篇下一篇

高效液相色谱法同时测定柑橘果实中18种类黄酮的含量

张元梅, 周志钦, 孙玉敬, 沈妍, 钟烈洲, 乔丽萍, 叶兴乾

1.西南大学园艺园林学院，重庆 400716
2.浙江大学生物系统工程与食品科学学院，杭州 310029

收稿日期:2012-04-11 出版日期:2012-09-01 发布日期:2012-06-19
通讯作者: 通信作者叶兴乾，E-mail：psu@zju.edu.cn
作者简介:张元梅，Tel：15923202230；E-mail：ymzhanggz@163.com
基金资助:
国家科技支撑计划项目（2012BAD31B06）、国家自然科学基金项目（31071635，31171930）、中国博士后科学基金第五十批面上项目（316000-X91102）

Simultaneous Determination of 18 Flavonoids in Citrus Fruits y High-Performance Liquid Chromatography

ZHANG Yuan-Mei, ZHOU Zhi-Qin, SUN Yu-Jing, SHEN Yan, ZHONG Lie-Zhou, QIAO Li-Ping, YE Xing-Qian

1.西南大学园艺园林学院，重庆 400716
2.浙江大学生物系统工程与食品科学学院，杭州 310029

Received:2012-04-11 Online:2012-09-01 Published:2012-06-19

摘要/Abstract

摘要： 【目的】建立一种高效液相色谱法同时测定柑橘果实中18种类黄酮（圣草次苷、二氢槲皮素、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、野漆苷、槲皮苷、圣草素、香峰草苷、枸橘苷、柚皮素、木犀草素、山奈酚、香叶木素、橙黄酮、川陈皮素、橘皮素）的含量。【方法】色谱柱为ZORABX SB-C18（4.6 mm×250 mm, 5 μm），流动相为甲醇-甲酸-水，梯度洗脱，流速为0.7 mL•min-1，柱温25℃，检测波长黄烷酮为283 nm、黄酮和多甲氧基黄酮为330 nm、黄酮醇为367 nm。【结果】 18种类黄酮在42 min内完全分离，线性范围为62.4—960 mg•L-1（r=0.9996—1.0000），定量限为0.05—0.133 μg•mL-1，回收率为88.70%—104.76%，相对0.33%—3.05%（n=6）。【结论】该方法简便、快速、准确、可靠，适合柑橘果实中18种类黄酮的含量测定。

关键词: 高效液相色谱, 柑橘, 类黄酮

Abstract: 【Objective】This study aimed to investigate a high-performance liquid chromatograph（HPLC）method for the determination of different kinds of flavonoids, namely, eriocitrin, taxifolin, narirutin, naringin, hesperidin, neohesperidin, rhoifolin, quercitrin, eridictyol, didymin, poncirin, naringenin, luteolin, kaempferol, diosmetin, sinensetin, nobiletin and tangeretin in citrus fruits. 【Method】The chromatographic separation was performed on a ZORABX SB-C18 column kept at 25℃ using methanol /formic acid/water mixture as the mobile phase at a flow rate of 0.7 mL•min-1 through gradient elution and the detection wavelength was set at 283 nm for flavanones, 330 nm for flavones and polymethoxylated flavones and 367 nm for flavonol. 【Result】 Results showed that the thorough separation of 18 flavonoids was achieved within 42 min. The method exhibited a linear range of 62.4-960 mg•L-1 (r=0.9998-1.0000) and limits of quantification (LOQ) ranging from 0.05-0.133 μg•mL-1. The average recovery rates of this method were 88.70%-104.76%, with a relative standard derivation range of 0.33%-3.05%. 【Conclusion】This method is simple, rapid, accurate , reliability, and can be used for the determination of flavonoids in citrus fruits.

Key words: high performance liquid chromatography, citrus fruit, flavonoids

张元梅, 周志钦, 孙玉敬, 沈妍, 钟烈洲, 乔丽萍, 叶兴乾. 高效液相色谱法同时测定柑橘果实中18种类黄酮的含量[J]. 中国农业科学, 2012, 45(17): 3558-3565.

ZHANG Yuan-Mei, ZHOU Zhi-Qin, SUN Yu-Jing, SHEN Yan, ZHONG Lie-Zhou, QIAO Li-Ping, YE Xing-Qian. Simultaneous Determination of 18 Flavonoids in Citrus Fruits y High-Performance Liquid Chromatography[J]. Scientia Agricultura Sinica, 2012, 45(17): 3558-3565.

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高效液相色谱法同时测定柑橘果实中18种类黄酮的含量

Simultaneous Determination of 18 Flavonoids in Citrus Fruits y High-Performance Liquid Chromatography

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