茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用

doi:10.3864/j.issn.0578-1752.2011.19.012

中国农业科学 ›› 2011, Vol. 44 ›› Issue (19): 4022-4028.doi: 10.3864/j.issn.0578-1752.2011.19.012

茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用

张立宁, 程继鸿, 杨瑞, 孙中华, 吴春霞, 王绍辉

1.北京农学院植物科学技术学院/农业应用新技术北京市重点实验室

收稿日期:2010-09-10 出版日期:2011-10-01 发布日期:2011-06-01
通讯作者: 通信作者王绍辉，E-mail：wangshaohui@bac.edu.cn
作者简介:张立宁，E-mail：ning_347@163.com
基金资助:
教育部科学技术研究重点项目（210001）、北京市自然基金项目（6092005）、北京市属高等学校人才强教计划项目（PHR200907136）

Effects of JA Synthesis-Related Genes Spr2 and LePrs on the

ZHANG Li-Ning, CHENG Ji-Hong, YANG Rui, SUN Zhong-Hua, WU Chun-Xia, WANG Shao-Hui

1.北京农学院植物科学技术学院/农业应用新技术北京市重点实验室

Received:2010-09-10 Online:2011-10-01 Published:2011-06-01

摘要/Abstract

摘要： 【目的】研究茉莉酸合成相关基因Spr2与LePrs对番茄抗根结线虫的影响，探讨茉莉酸在番茄抗根结线虫病中的作用。【方法】以番茄茉莉酸合成突变体spr2、茉莉酸过量表达转基因番茄35S::PS及野生型CM为试材，研究外源喷施茉莉酸甲酯及不同番茄试材相互嫁接对接种根结线虫后番茄根结数、蛋白质酶抑制剂PI-II表达及Spr2、LePrs基因转录水平的影响。【结果】番茄茉莉酸合成突变体spr2较野生型与茉莉酸过量表达转基因番茄35S::PS容易感染根结线虫，外源喷施茉莉酸甲酯降低了番茄根结数。以茉莉酸过量表达转基因番茄35S::PS为砧木，可以提高嫁接植株对根结线虫的抵抗能力。【结论】Spr2突变降低了番茄对根结线虫的抗性， LePrs过量表达可以提高植株对根结线虫的抗性。因此，茉莉酸在番茄抗根结线虫病中起到了一定的作用。

关键词:

番茄茉莉酸突变体, 南方根结线虫, PI-II蛋白酶抑制剂, Spr2, LePrs

Abstract: 【Objective】 Spr2 and LePrs were related with JA synthesis, the effects of the two genes on the response of tomato to root-knot nematodes were studied. 【Method】 In this paper, JA biosynthetic mutant (spr2 plants) and JA-overexpression transgenic plants (35S::PS plants) of tomato as well as wild tomato species (CM) were used, and the effects of MeJA sprays and grafting on root-knot numbers, PI-II content and Spr2, LePrs transcription levels, before and after inoculating root-knot nematodes, were analyzed. 【Result】 Compared with CM and 35S::PS plants, spr2 plants was more susceptible to root-knot nematodes. Spraying MeJA on tomato leaves could reduce root-knot numbers. Using 35S::PS plants as stock improved grafting tomato resistance to root-knot nematodes. 【Conclusion】 Resistance to nematodes in JA biosynthetic mutant (with mutant Spr2) was reduced, while in JA-overexpression transgenic tomato (with over-expressing LePrs) it was increased. So Jasmonic acid played a role in the response of tomato to root-knot nematodes.

Key words:

tomato, root-knot nematodes, jasmonic acid, graft, LePrs, Spr2

张立宁, 程继鸿, 杨瑞, 孙中华, 吴春霞, 王绍辉. 茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用[J]. 中国农业科学, 2011, 44(19): 4022-4028.

ZHANG Li-Ning, CHENG Ji-Hong, YANG Rui, SUN Zhong-Hua, WU Chun-Xia, WANG Shao-Hui. Effects of JA Synthesis-Related Genes Spr2 and LePrs on the[J]. Scientia Agricultura Sinica, 2011, 44(19): 4022-4028.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.19.012

https://www.chinaagrisci.com/CN/Y2011/V44/I19/4022

参考文献

[1]Staswick P E, Yuen G Y, Lehman C C. Jasmonate signaling mutants of Arabidopsis are susceptible to the soil fungus Pythium irregulare. The Plant Journal, 1998, 15:747-754.

[2]Vijayan P, Shockey J, Levesque C A, Cook R J, Browse J. A role for jasmonate in pathogen defense of Arabidopsis. Proceedings of the National Academy of Sciences of the USA, 1998, 95: 7209-7214.

[3]Thaler J S, Owen B, Higgins V J. The role of the jasmonate response in plant susceptibility to diverse pathogens with a range of lifestyles. Plant Physiology, 2004, 135: 530-538.

[4]Kessler A, Halitschke R, Baldwin I T. Silencing the jasmonate cascade: induced plant defenses and insect populations. Science, 2004, 305: 665-668.

[5]Creelman R A, Mullet J E. Oligosaccharins, brassinolides, and jasmonates: nontraditional regulators of plant growth, development, and gene expression. The Plant Cell, 1997, 9: 1211-1223.

[6]Farmer E E, Ryan C A. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proceedings of the National Academy of Sciences of the USA, 1990, 87: 7713-7716.

[7]Cooper W R, Jia L, Goggin L. Effects of jasmonate-induced defenses on root-knot nematode infection of resistant and susceptible tomato cultivars. Journal of Chemical Ecology, 2005, 31: 1953-1967.

[8]Farmer E E, Ryan C A. Octadecanoid precursors of jasmonic acid activate the synthesis of wounding-inducible proteinase inhibitors. The Plant Cell, 1992, 4(2): 129-134.

[9]Thaler J S. Induced resistance in agricultural crops: Effects of jasmonic acid on herbivory and yield in tomato plants. Environmental Entomology, 1999, 99: 30-37.

[10]王绍辉, 孔云, 杨瑞, 程继鸿, 司力珊, 赵金芳. 嫁接番茄抗根结线虫砧木筛选及抗性研究. 中国蔬菜, 2008(12): 24-27.

Wang S H, Kong Y, Yang R, Cheng J H, Si L S, Zhao J F. Studies on rootstock screening and resistance to root-knot nematodes for grafted tomato. China Vegetables, 2008(12): 24-27. (in Chinese)

[11]Nicola V, Rapoport H F, Rafael M, Pablo C. Differences in feeding sites induced by root-knot nematodes, Meloidogyne spp., in chickpea. The American Phytopathological Society, 2005, 95(4): 368- 375.

[12]徐小明. 茄子砧木对南方根结线虫抗性鉴定及抗性机制研究[D].山东泰安: 山东农业大学, 2008.

Xu X M. Study on evaluation for resistances to Meloidogyne incognita and the resistant mechanism of eggplant rootstocks [D]. Taian, Shandong: Shandong Agriculture of University, 2008. (in Chinese)

[13]萨姆布鲁克 J, 拉塞尔D W. 分子克隆实验指南.第3 版. 北京: 科学技术出版社, 2001: 1236-1239.

Sambrook J, Russell D W. Molecular Cloning a Laboratory Manual. 3rd ed. Beijing: Technology Science Publishing Company, 2001: 1236-1239. (in Chinese)

[14]Li C B, Zhao J H, Jiang H L, Wu X Y, Sun J Q, Zhang C Q, Wang X, Lou Y G, Li C Y. The wound-response mutant suppressor of prosystemin-mediated responses6 (spr6) is a weak allele of the tomato homolog of CORONATINE-INSENSITIVE1 (COI1). Plant Cell Physiology, 2006, 47: 653-663.

[15]Li C Y, Liu G H, Xu C C, Lee G I, Peter B, Hong Q L, Martin W G, Gregg A H. The tomato suppressor of prosystemin-mediated responses2 gene encodes a fatty acid desaturase required for the biosynthesis of jasmonic acid and the production of a systemic wound signal for defense gene expression. The Plant Cell, 2003, 15 (7): 1646-1661.

[16]McGurl B, Pearce G, Orozco-Cardenas M and Ryan C A. Structure, expression, and antisense inhibition of the systemin precursor gene. Science, 1992, 255: 1570-1573.

[17]McGurl B, Orozco-Cárdenas M, Pearce G, Ryan C A. Overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis. Proceedings of the National Academy of Sciences of the USA, 1994, 91(10): 9799-9802.

[18]Ryan C A. Proteinase inhibitors in plants, genes for improving defenses against insects and pathogens. Annual Review Phytopathology, 1990, 28: 425-449.

[19]Schaller F. Enzymes of the biosynthesis of octadecanoid-derived signaling molecules. Journal of Experimental Botany, 2001, 52: 11-23.

[20]Botella M A, Xu Y, Prabha T N, Zhao Y, Narasimhan M L, Wilson K A, Nielsen S S, Bressan R A, Hasegasa P M. Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. Plant Physiology, 1996, 112: 1201-1210.

[21]Dammann C, Rojo E, Sanchez S J J. Abscisic acid and jasmonic acid activate wound-inducible genes in potato through separate, organ-specific signal transduction pathways. The Plant Journal, 1997, 11: 773-782.

[22]Vandam N M, Horn M, Mares M, Baldwin L T. Ontogeny constrains systemic protease inhibitor response in Nicotiana attenuata. Journal of Chemical Ecology, 2001, 27: 547-568.

[23]Tsao R, Zhou T. Interaction of monoterpenoids, methyljasmonate, and Ca2+ in controlling post-harvest brown rot of sweet cherry. HortScience, 2000, 35(7): 1304-1307.

[24]Redman A M, Cipollini D F J, Schultz J C. Fitness costs of jasmonic acid in defense in tomato, Lycopersicon esculentum. Oecologia, 2001, 126: 380-385.

[25]Cohen Y, Gisi U, Niderman T. Local and systemic protection against phytophthora infestans induced in potato and tomato plants by jasmonic acid and jasmonic methyl ester. Phytopathology, 1993, 83: 1054-1062.

[26]Howe G A, Ryan C A. Suppressors of system in signaling identify genes in the tomato wound response pathway. Genetics, 1999, 153: 1411-1421.

[27]Li X C, Schuler M A, Berenbaum M R. Jasmonate and salicylate induce expression of herbivore cytochrome P450 genes. Nature, 2002, 419: 712-715.

[28]Koh A, Nobuo S, Shu F, Naoshi D, KeikoY, Toru F, Hiroaki, H, Tomoyuki Y, Hitoshi S. Destination-selective long-distance movement of phloem proteins. The Plant Cell, 2005, 17: 1801-1814.

[29]Brian G A, Robert T. Graft Transmission of a floral stimulant derived from CONSTANS. Plant Physiology, 2004, 135: 2271-2278.

[30]Minsung K, Wynnelena C, Sharon K, Neelima S. Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. Science, 2001, 293: 287-289.

茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用

Effects of JA Synthesis-Related Genes Spr2 and LePrs on the

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

Metrics

本文评价

推荐阅读 0

[1]	王刚, 李二峰, 茆振川, 谢丙炎, 冯东昕. 对辣椒抗性基因Me3表现毒性的南方根结线虫群体的SCAR分子标记[J]. 中国农业科学, 2013, 46(5): 943-949.
[2]	曹君正, 武侠, 林森. 刀孢蜡蚧菌的鉴定及其对南方根结线虫不同生活阶段的定殖[J]. 中国农业科学, 2012, 45(12): 2404-2411.
[3]	叶德友, 钱春桃, 陈劲枫. 酸黄瓜对南方根结线虫抗性的光合响应[J]. 中国农业科学, 2011, 44(20): 4248-4257.
[4]	贾双双,高荣广,徐坤 . 番茄砧木对南方根结线虫抗性鉴定[J]. 中国农业科学, 2009, 42(12): 4301-4307 .
[5]	张成敏,武侠,才秀华. 厚垣普奇尼亚菌Pochonia chlamydosporia产生的几丁质酶对南方根结线虫卵孵化的影响 [J]. 中国农业科学, 2009, 42(10): 3509-3515 .