小麦籽粒蛋白质含量的动态QTL定位

doi:10.3864/j.issn.0578-1752.2011.15.002

摘要/Abstract

摘要： 【目的】检测灌浆过程中控制小麦籽粒蛋白质含量（GPC）的条件及非条件QTL，阐明不同时期及不同时段内QTL的表达方式，揭示籽粒蛋白质积累的分子遗传机理。【方法】以花培3号×豫麦57的168个双单倍体（doubled haploid，DH）群体为材料，于6个不同的环境下种植，在籽粒灌浆的5个时期取样，对小麦GPC进行动态QTL分析。【结果】共检测到影响GPC的9个非条件QTL和10个条件QTL。QGpc3A为整个灌浆过程都能表达的非条件QTL，其余条件和非条件QTL只在几个或单独一个时期表达。花后12 d，控制GPC的基因表达活跃，非条件QTL和条件QTL总共能解释表型变异贡献率的42.62%；花后22 d，条件QTL和非条件QTL总共可解释表型变异的贡献率较低，仅为17.43%，GPC降到“低谷”。 QGpc4A-1对GPC前期积累有重要意义，QGpc1D和QGpc4A-2对GPC灌浆中后期积累有重要意义。【结论】GPC呈现出“高-低-高”的变化规律，控制GPC的基因在灌浆过程中以一定的时空方式表达。

关键词: 普通小麦, DH群体, 籽粒蛋白质含量（GPC）, 非条件QTL, 条件QTL

Abstract: 【Objective】Grain protein content (GPC) is an important quality factor in bread wheat, which is determined by quantitative trait loci (QTLs). In this study, to attain more genetic information of GPC accumulation，the number and genetic effects of QTLs/genes related to GPC were detected at different seed filling stages. 【Method】 QTLs/genes related to GPC were studied at five seed filling stages in six different environments, using a doubled haploid (DH) population derived from a cross between two elite Chinese wheat cultivars Huapei 3×Yumai 57. QTL mapping for developmental behavior of GPC was studied by unconditional and conditional quantitative trait locus (QTL) analyses in a mixed linear model.【Result】A total of nine unconditional QTLs and ten conditional QTLs were detected at five seed filling periods. The unconditional QTL named QGpc3A persistently expressed at all seed filling stages may play a most important role for the accumulation of GPC, the rest unconditional QTLs and conditional QTLs were only detected at a few or a special periods. At the period of 12 days after anthesis the gene expression was active, and the QTLs detected could account for 42.62% of phenotypic variation totally. However, the QTLs detected could totally account for only 17.43% of phenotypic variation at 22 d after anthesis with lowest phenotypic value. 【Conclusion】The dynamic change of GPC show a high - low - high trend and QTLs controlling GPC were developmental stage speci?c.

Key words: common wheat, DH population, grain protein content (GPC), unconditional QTL, conditional QTL

朱占玲, 刘宾, 田宾, 谢全刚, 李文福, 田纪春. 小麦籽粒蛋白质含量的动态QTL定位[J]. 中国农业科学, 2011, 44(15): 3078-3085.

ZHU Zhan-Ling, LIU Bin, TIAN Bin, XIE Quan-Gang, LI Wen-Fu, TIAN Ji-Chun. Dynamic QTL Mapping of Wheat Protein Content in Developing Grains[J]. Scientia Agricultura Sinica, 2011, 44(15): 3078-3085.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.15.002

https://www.chinaagrisci.com/CN/Y2011/V44/I15/3078

参考文献

[1]Blanco A, De Giovanni C, Laddomada B, Sciancalepore A, Simeone R, Devos K M, Gale M D. Quantitative trait loci influencing grain protein content in tetraploid wheats. Plant Breeding, 1996, 115: 310-316.

[2]Mesfin A, Frohberg R C, Anderson J A. RFLP markers associated with high grain protein from Triticum turgidum L. var. dicoccoides introgressed into hard red spring wheat. Crop Science, 1999, 39: 508-513.

[3]Harjit S, Prasad M, Varshney R K, Roy J K. Balyan H S, Gupta P K. STMS markers for grain protein content and their validation using near-isogentic lines in bread wheat. Plant Breeding, 2001, 120(4): 273-278.

[4]Prasad M, Kumar N, Kulwal P L, Röder M S, Balyan H S, Dhaliwal H S, Gupt. P K. QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theoretical and Applied Genetics, 2003, 106: 659-667.

[5]Huang X Q, Cloutier S, Lycar L, Radovanovic N, Humphreys D G, Noll J S, Somers D J, Brown P D. Molecular detection of QTL for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theoretical and Applied Genetics, 2006, 113: 753-766.

[6]石培春, 曹连莆, 王光利, 张薇. 小麦籽粒蛋白质组分含量的QTL定位. 麦类作物学报, 2008, 28(4): 550-554.

Shi P C, Cao L P, Wang G L, Zhang W. Mapping QTLs related to prptein components of grain of common wheat. Journal of Triticeae Crops, 2008, 28(4): 550-554. (in Chinese)

[7]Zhao L, Zhang K P, Liu B, Deng Z Y, Qu H L, Tian J C. A comparison of grain protein content QTLs and ?our protein content QTLs across environments in cultivated wheat. Euphytica, 2010, 174: 325-335.

[8]Yan J Q, Zhu J, He C X, Benmoussa1 M, Wu P. Moleculai dissection of developmental behavior of plant height in rice (Oryza sativa L. ). Genetics, 1998, 150: 1257-1265.

[9]吴为人, 李维明, 卢浩然. 数量性状基因座的动态定位策略. 生物数学学报, 1997, 12(5): 490-498.

Wu W R, Li W M, Lu H R. Strategy of dynamic mapping of quantitative trait loci. Journal of Biomathematics, 1997, 12(5): 490-498. (in Chinese)

[10]Zhu J. Analysis of conditional genetic effects and variance components in developmental genetics. Genetics, 1995, 141: 1633-1639.

[11]何慈信, 朱军, 严菊强, Mebrouk B, 吴平. 水稻穗干物质重发育动态的QTL定位. 中国农业科学, 2000, 33(1): 24-32.

He C X, Zhu J, Yan J Q, Benmoussa M, Wu P. QTL mapping for developmental behavior of panicle dry weight in rice. Scientia Agricultura Sinica, 2000, 33(1): 24-32. (in Chinese)

[12]何慈信, 朱军, 严菊强, Mebrouk Benmoussa, 吴平. 水稻叶挺长发育动态的QTL 分析. 中国水稻科学, 2000, 14(4): 193-198.

He C X, Zhu J, Yan J Q, Benmoussa M, Wu P. QTL mapping for development al behaviour of leaf sheat h height in rice. Chinese Journal Rice Science, 2000, 14(4): 193-198. (in Chinese)

[13]Liu G F, Zhu H T, Liu S W, Zeng R Z, Zhang Z M, Li W T, Ding X H, Zhao F M, Zhang G Q. Unconditional and conditional QTL mapping for the developmental behavior of tiller number in rice (Oryza sativa L.). Genetica, 2010, 138: 885-893.

[14]Qu Y Y, Mu P, Zhang H L, Chen C Y, Gao Y M, Tian Y Y, Wen F, Li Z C. Mapping QTLs of root morphological traits at different growth stages in rice. Genetica, 2008, 133: 187-200.

[15]严建兵, 汤华, 黄益勤, 石永刚, 李建生, 郑用琏. 不同发育时期玉米株高QTL的动态分析. 科学通报, 2003, 48(18): 1959-1964.

Yan J B, Tang H, Huang Y Q, Shi Y G, Li J S, Zheng Y L. QTL mapping for the developing of the plant height traits in maize. Chinese Science Bulletin, 2003, 48(18): 1959-1964. (in Chinese)

[16]崔世友, 喻德跃. 大豆不同生育时期叶绿素含量QTL的定位及其与产量的关联分析. 作物学报, 2007, 33(5): 744-750.

Cui S Y, Yu D Y. QTL mapping of chlorophyll content at various growing stages and its relationship with yield in soybean [Glycine max(L.) Merr.]. Acta Agronomica Sinica, 2007, 33(5): 744-750. (in Chinese)

[17]韩英鹏, 滕卫丽, 杜玉萍, 孙德生, 张忠臣, 徐香玲. 不同发育时期大豆籽粒干物质积累的QTL动态分析. 中国农业科学, 2010, 43(7): 1328-1338.

Han Y P, Teng W L, Du Y P, Sun D S, Zhang Z C, Xu X L. Dynamic QTL Analysis of dry matter accumulation in soybean seed at different developmental stages. Scientia Agricultura Sinica, 2010, 43(7): 1328-1338. (in Chinese)

[18]Zhang K P, Fang Z J, Liang Y, Tian J C. Genetic dissection of chlorophyⅡ content at different growth stages in common wheat. Journal of Genetics, 2009, 88: 183-189.

[19]刘宾, 赵亮, 张坤普, 朱占玲, 田宾, 田纪春. 小麦株高发育动态QTL定位. 中国农业科学, 2010, 43(22): 4562-4570.

Liu B, Zhao L, Zhang K P, Zhu Z L, Tian B, Tian J C. Genetic dissection of plant height at different growth stages in common wheat. Scientia Agricultura Sinica, 2010, 43(22): 4562-4570. (in Chinese)

[20]Wu X S, Wang Z H, Chang X P, Jing R L. Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes. Journal of Experimental Botany, 2010, 61(11): 2923-2937.

[21]李卫华, 尤明山, 刘伟, 徐杰, 刘春雷, 李保云, 刘广田. 小麦GMP含量发育动态的QTL定位. 作物学报, 2006, 32(7): 995-1000.

Li W H, You M S, Liu W, Xu J, Liu C L, Li B Y, Liu G T. QTL mapping for developmental behavior of GMP content in wheat. Acta Agronomica Sinica, 2006, 32(7): 995-1000. (in Chinese)

[22]刘丽, 李卫华, 刘伟, 曹连莆, 李保云, 刘广田. 小麦谷蛋白膨胀指数发育动态的QTL分析. 中国农业科学, 2008, 41(11): 3838-3844.

Liu L, Li W H, Liu W, Cao L P, Li B Y, Liu G T. Analysis of QTL for SIG at different developmental stages in wheat. Scientia Agricultura Sinica, 2008, 41(11): 3838-3844. (in Chinese)

[23]Zhang K P, Tian J C, Zhao L, Wang S S. Mapping QTLs with epistatic effects and QTL×environment interactions for plant height using a doubled haploid population in cultivated wheat. Journal of Genetics and Genomics, 2008, 35: 119-127.

[24]朱军. 运用混合线性模型定位复杂数量性状基因的方法. 浙江大学学报: 工学版, 1999, 33(3): 327-335.

Zhu J. Mixed model approaches of mapping genes for complex quantitative traits. Journal of Zhejiang University: Engineering Science, 1999, 33(3): 327-335. (in Chinese)

[25]Yang J, Zhu J. Methods for predicting superior genotypes under multiple environments based on QTL effects. Theoretical and Applied Genetics, 2005, 110: 1268-1274.

[26]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genetics Newsletter, 1997, 14: 11-13.

[27]王月福, 于振文, 李尚霞, 余松烈. 氮素营养水平对冬小麦氮代谢关键酶活性变化和籽粒蛋白质含量的影响. 作物学报, 2002, 28(6): 743-748.

Wang Y F, Yu Z W, Li S X, Yu S L. Effect of nitrogen nutrition on the change of key enzyme activity during the nitrogen metabolism and kernel protein content in winter wheat. Acta Agronomica Sinica, 2002, 28(6): 743-748. (in Chinese)

[28]王月福, 姜东, 于振文, 曹卫星. 氮素水平对小麦籽粒产量和蛋白质含量的影响及生理基础. 中国农业科学, 2003, 36(5): 513-520.

Wang Y F, Jiang D, Yu Z W, Cao W X. Effects of nitrogen rates on grain yield and protein content of wheat and its physiological basis. Scientia Agricultura Sinica, 2003, 36(5): 513-520. (in Chinese)

[29]朱新开, 周君良, 封超年, 郭文善, 彭永欣. 不同类型专用小麦籽粒蛋白质及其组分含量变化动态差异分析. 作物学报, 2005, 31(3): 342-347.

Zhu X K, Zhou J L, Feng C N, Guo W S, Peng Y X. Differences of protein and its component accumulation in wheat for different end uses. Acta Agronomica Sinica, 2005, 31(3): 342-347. (in Chinese)

[30]杨延兵, 高荣岐, 尹艳枰, 管延安, 张华文. 不同基因型小麦籽粒蛋白质合成动态及相关酶活性的研究. 华北农学报, 2007, 22(3): 43-47.

Yang Y B, Gao R Q, Yin Y P, Guan Y A, Zhang H W. Studies on the dynamic changes of protein synthesis and the related enzymes in different genotype wheat. Acta Agriculturae Boreali-Sinica, 2007, 22(3): 43-47. (in Chinese)

[31]孙德生, 李文滨, 张忠臣, 陈庆山, 杨庆凯. 大豆株高QTL发育动态分析. 作物学报, 2006, 32(4): 509-514.

Sun D S, Li W B, Zhang Z C, Chen Q S, Yang Q K. Analysis of QTL for plant height at different development stages in soybean. Acta Agronomica Sinica, 2006, 32(4): 509-514. (in Chinese)