小麦品种偃展1号与品系早穗30重组自交系群体遗传连锁图谱构建及重要农艺性状的QTL分析

doi:10.3864/j.issn.0578-1752.2010.20.002

中国农业科学 ›› 2010, Vol. 43 ›› Issue (20): 4130-4139 .doi: 10.3864/j.issn.0578-1752.2010.20.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

小麦品种偃展1号与品系早穗30重组自交系群体遗传连锁图谱构建及重要农艺性状的QTL分析

姚琴,周荣华,潘昱名,傅体华,贾继增

(四川农业大学农学院)

收稿日期:2010-07-07 修回日期:2010-08-13 出版日期:2010-10-15 发布日期:2010-10-15
通讯作者: 傅体华,贾继增

Construction of Genetic Linkage Map and QTL Analysis of Agronomic Important Traits Based on a RIL Population Derived from Common Wheat Variety Yanzhan 1 and Zaosui 30

YAO Qin, ZHOU Rong-hua, PAN Yu-ming, FU Ti-hua, JIA Ji-zeng

(四川农业大学农学院)

Received:2010-07-07 Revised:2010-08-13 Online:2010-10-15 Published:2010-10-15
Contact: FU Ti-hua, JIA Ji-ze

摘要/Abstract

摘要：

【目的】构建重组自交系(recombinant inbred line,RIL)群体及其遗传连锁图谱,对小麦重要农艺性状进行数量性状位点(quantitative trait locus,QTL)分析,为发现小麦新基因与分子标记辅助育种奠定基础。【方法】配制普通小麦品种(系)早穗30和偃展1号的杂交组合,通过一粒传的方法培育重组自交系群体;利用SSR(simple sequence repeat)标记、DarT(diversity arrays technology)标记、ISBP(insertion site-based polymorphism)标记以及抽穗期和株高的功能标记绘制其遗传连锁图谱并通过复合区间作图法(Composite interval mapping,CIM)对多个环境下的抽穗期、株高、千粒重、穗粒数、每穗小穗数、穗长等农艺性状进行QTL定位分析。【结果】培育出由219个F7家系组成的重组自交系群体;构建了含481个分子标记的遗传连锁图谱;检测出分布在12条染色体上的26个与重要农艺性状相关的QTL,其中9个QTL能够在至少2个环境下重复;研究还发现了3个QTL聚集的“QTL簇”,其中4D染色体上的矮秆基因Rht2所在区段控制株高与千粒重,5D染色体上的Vrn-D1—WMS212区间控制抽穗期、穗粒数与每穗小穗数,7B染色体上wPt4230—wPt4814区段控制抽穗期、穗粒数、株高与穗长。【结论】构建的小麦遗传作图群体可成功地用于重要农艺性状分析;矮秆基因Rht2与春化基因Vrn-D1 2个发育相关基因均与多个重要农艺性状有关;在7B上可能存在与发育相关的重要新基因。

关键词: 小麦, 分子标记, QTL

Abstract:

【Objective】 The present study was aimed to develop a RIL (recombinant inbred line) population, construct its genetic map and analyze quantitative trait loci (QTL) associated with important agronomic traits in wheat. This work will accelerate gene discovery and marker-assistant breeding of wheat. 【Method】 A RIL population was derived from the cross of Yanzhan1 and Zaosui 30 by single seed descent approach. SSR (simple sequence repeat), DarT (diversity arrays technology), ISBP (insertion site-based polymorphism) and functional makers associated with days to heading (DH) and plant height (PH) were used to construct genetic map. QTLs related to DH, PH, thousand grain weight (TGW), grain number per spike (GNS), spike length (SL) and spikelet numbers (SPI) in different environments were detected by using the composite interval mapping (CIM). 【Result】 A set of 219 F7 RILs was derived. A genetic linkage map with 481 molecular markers covering the whole wheat genome was constructed. A total of 26 QTLs for the related traits located on 12 chromosomes were detected, among which 9 QTLs were detected in at least two environments. Three QTL clusters were revealed including one cluster for PH and TGW located on dwarf gene Rht2 region on 4DS, one cluster for DH, GNS and SPI located in region Vrn-D1-WMS212 of chromosome 5D and the other cluster for DH, SPI, PH, and SL located in the interval of wPt4230 and wPt4814 on chromosome 7B. 【Conclusion】 The genetic map is favorable to analyze important agronomic traits. Dwarfing gene Rht2 and vernalization gene Vrn-D1, two developmental related genes, are related to some important agronomic traits. There might be a novel gene concerning plant development on chromosome 7B.

Key words: wheat, molecular marker, QTL

姚琴,周荣华,潘昱名,傅体华,贾继增 . 小麦品种偃展1号与品系早穗30重组自交系群体遗传连锁图谱构建及重要农艺性状的QTL分析
[J]. 中国农业科学, 2010, 43(20): 4130-4139 .

YAO Qin,ZHOU Rong-hua,PAN Yu-ming,FU Ti-hua,JIA Ji-zeng
. Construction of Genetic Linkage Map and QTL Analysis of Agronomic Important Traits Based on a RIL Population Derived from Common Wheat Variety Yanzhan 1 and Zaosui 30
[J]. Scientia Agricultura Sinica, 2010, 43(20): 4130-4139 .

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https://www.chinaagrisci.com/CN/Y2010/V43/I20/4130

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小麦品种偃展1号与品系早穗30重组自交系群体遗传连锁图谱构建及重要农艺性状的QTL分析

Construction of Genetic Linkage Map and QTL Analysis of Agronomic Important Traits Based on a RIL Population Derived from Common Wheat Variety Yanzhan 1 and Zaosui 30

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