中国农业科学 ›› 2010, Vol. 43 ›› Issue (3): 529-534 .doi: 10.3864/j.issn.0578-1752.2010.03.012

• 园艺 • 上一篇    下一篇

光质对黄瓜叶片衰老与抗氧化酶系统的影响

王虹,姜玉萍,师恺,周艳虹,喻景权

  

  1. (浙江大学园艺系)
  • 收稿日期:2009-05-04 修回日期:2009-07-16 出版日期:2010-02-10 发布日期:2010-02-10
  • 通讯作者: 喻景权

Effects of Light Quality on Leaf Senescence and Activities of Antioxidant Enzymes in Cucumber Plants

WANG Hong, JIANG Yu-ping, SHI Kai, ZHOU Yan-hong, YU Jing-quan
  

  1. (浙江大学园艺系)
  • Received:2009-05-04 Revised:2009-07-16 Online:2010-02-10 Published:2010-02-10
  • Contact: YU Jing-quan

摘要:

【目的】明确不同光质对黄瓜叶片衰老的作用及其机制。【方法】以黄瓜为材料,研究白光(W)、紫光(P)、蓝光(B)、绿光(G)、黄光(Y)和红光(R)等不同光质对叶片叶绿素、可溶性蛋白及丙二醛(MDA)的含量和CAT、G-POD、APX等抗氧化酶的活性及基因表达的影响。【结果】紫光和蓝光诱导了抗氧化酶基因的表达及活性的上升,延缓了叶绿素和可溶性蛋白含量的下降,并且使MDA的含量保持相对较低的水平,从而延缓了植株的衰老。绿光、黄光和红光则抑制了抗氧化酶的活性,导致黄瓜植株叶绿素和可溶性蛋白含量的不断下降及MDA含量的持续上升,加速了植株的衰老进程。【结论】紫光和蓝光可以使叶片维持较高的抗氧化酶水平,从而延缓了植株的衰老。

关键词: 黄瓜, 光质, 抗氧化酶, 衰老

Abstract:

【Objective】 To investigate the mechanism involved in the different responses of leaf senescence to light qualities, cucumber (Cucumis sativus L.) seedlings were exposed to white (W), purple (P), blue (B), green (G), yellow (Y), and red (R) lights for 15 d. 【Method】 The contents of chlorophyll, soluble proteins and MDA together with the expression and activities of antioxidant enzymes were measured 【Result】 P and B increased the transcript levels and activities of antioxidant enzymes, the contents of chlorophyll, soluble proteins and MDA. In contrast, G, Y and R decreased the activities of antioxidant enzymes, the contents of chlorophyll, soluble proteins and MDA. 【Conclusion】 These results indicate that P and B prevented senescence by maintaining high levels of antioxidant enzymes.

Key words: Cucumis sativus L, light quality, antioxidant enzymes, senescence