中国农业科学 ›› 2010, Vol. 43 ›› Issue (3): 468-473 .doi: 10.3864/j.issn.0578-1752.2010.03.004

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于性状和分子标记的高丹草近等基因系的分离研究

逯晓萍,云锦凤,米福贵,陈强,张雅慧,薛春雷

  

  1. (内蒙古农业大学农学院遗传育种教研室)
  • 收稿日期:2008-10-26 修回日期:2009-10-17 出版日期:2010-02-10 发布日期:2010-02-10
  • 通讯作者: 云锦凤

Segregation Based on the Traits and Molecular Marker of Near-Isogenic Line of Sorghum bicolor×Sorghum sudanenes

LU Xiao-ping, YUN Jin-feng, MI Fu-gui, CHEN Qiang, ZHANG Ya-hui, XUE Chun-lei
  

  1. (内蒙古农业大学农学院遗传育种教研室)
  • Received:2008-10-26 Revised:2009-10-17 Online:2010-02-10 Published:2010-02-10
  • Contact: YUN Jin-feng

摘要:

【目的】以株高等表型性状和分子标记为基础,从高粱×苏丹草的F6:7重组自交系群体中分离株高、叶片数2个性状及对这2个性状有效应的分子标记的近等基因系,为精确定位性状的QTL及分析QTL的作用提供有用的遗传材料。【方法】利用高粱314A×棕壳苏丹草的F2:3遗传作图群体的后代材料建立重组自交系群体F6:7,对株高、叶片数2个性状,采用基于性状和基于分子标记的近等基因系分离法,分别找出差异显著的株系和杂合的株系。将2种方法找出的共同株系再进行SSR标记,剔除杂合株,其余2种纯合子基因型即为该性状的近等基因系(NIL)。【结果】在重组自交系RIL-105和RIL-85家系内分别分离出了株高和叶片数的近等基因系。在重组自交系RIL-127家系内分离出了互作位点umc1714—umc2247的近等基因系。【结论】利用分离得到的这些近等基因系可进一步精确定位性状的QTL位点和分离QTL位点及对互作位点(上位性)的遗传效应进行分析。

关键词: 高丹草, 重组自交系, 近等基因系, 数量性状基因位点(QTLs), 上位性

Abstract:

【Objective】 Based on the traits and the molecular markers of Sorghum (Sorghum bicolor)× Sudan grass (Sorghum sudanenes), the near-isogenic lines (NILs) of plant height, leaf number and related molecular markers were determined from the recombinant inbred line (RIL) F6:7 family. The material could be used for the QTLs analysis. 【Method】 The recombinant inbred line F6:7 family came from F2:3 genetic mapping population of Sorghum314A×Brown Sudan grass,to the traits of plant height and leaf number, the NILs were isolated separately based on the traits and the molecular markers. The plants with one trait difference (P≤0.05) and with heterozygous lines were selected firstly, assisted with SSR marker, deleted the heterozygous plant, two kinds of homozygote became the NILs of the traits. 【Result】 The NILs of plant height and leaf number were isolated from RIL-105 and RIL-85 separately. The NIL with umc1714-umc2247 interaction loci came from RIL-127. 【Conclusion】 The NILs would be useful to the research of the trait detailed location and isolation and interaction (epistasis) of QTLs.

Key words: Sorghum×Sudan grass, recombinant inbred line, near-isogenic line, quantitative trait loci (QTLs), epistasis